China aims to reduce carbon dioxide emissions and achieve peak carbon and carbon neutrality goals. Natural gas, as a high-quality fossil fuel energy, is an important transition resource for China in ...the process of carbon reduction, so it is necessary to predict China’s natural gas demand. In this paper, a novel natural gas demand combination forecasting model is constructed to accurately predict the future natural gas demand. The Lasso model and the polynomial model are used to build a combinatorial model, which overcomes the shortcomings of traditional models, which have low data dimensions and poor prediction abilities. In the modeling process, the cross-validation method is used to adjust the modeling parameters. By comparing the performance of the combinatorial forecasting model, the single forecasting model and other commonly used forecasting models, the results show that the error (2.99%) of the combinatorial forecasting model is the smallest, which verifies the high accuracy and good stability advantages of the combinatorial forecasting model. Finally, the paper analyzes the relevant data from 1999 to 2022 and predicts China’s natural gas demand in the next 10 years. The results show that the annual growth rate of China’s natural gas demand in the next 10 years will reach 13.33%, at 8.3 × 1011 m3 in 2033, which proves that China urgently needs to rapidly develop the gas supply capacity of gas supply enterprises. This study integrates the impact of multiple factors on the natural gas demand, predicts China’s natural gas demand from 2023 to 2033, and provides decision-making support for China’s energy structure adjustment and natural gas import trade.
We report two novel electrochemical sensors (E-sensors) for the detection of target DNA and miRNA. The E-sensors were fabricated using label-free functional allosteric molecular beacons (aMBs), which ...can form streptavidin aptamers to bind to streptavidin peroxidase polymer and so generate catalytic currents in the presence of the targets. These E-sensors eliminate the antigen antibody interactions which require sophisticated DNA modification. During the experiment, we found a pair of CV peaks located at around 0.17V. These peaks contributed to the redox reaction between TMB and TMB+, and the adsorption–desorption process of TMB+ to the negative aMB backbone. When the E-sensor was hybridized with the complement of the aMB sequence, a pair of CV peaks were found at around 0.47V which were related to the redox reaction between TMB+ and TMB2+, and the process of intercalation of the planar structure of TMB2+ to dsDNA. The RSV-aMB E-sensor could detect 44amol RSV DNA in the 4μL sample and performed well in complicated biological environments. The let-7a-aMB E-sensor reached a detection limit of 13.6amol let-7a miRNA in the 4μL sample and showed good selectivity for one base mismatched miRNA.
► Two E-sensors were fabricated for RSV DNA and let-7a microRNA detection. ► The adsorption–desorption process between TMB+ and DNA was observed by CVs. ► The RSV-aMB E-sensor performed well in complicated biological environments. ► The RSV-aMB E-sensor could detect 44amol RSV DNA in the 4μL sample. ► The let-7a-aMB E-sensor could detect 13.6amol targets in the 4μL sample.
To alleviate pollution and promote clean energy production, food waste can be used to produce methane through anaerobic digestion (AD). In this study, wheat straw was used to produce biochar (BC) and ...magnetic biochar (MB) as additives for AD of food waste. MB2.5% and BC2.5% led to the highest methane production. The main action mechanism of the additives in AD was the change in environmental factors upon stimulating microorganisms to rapidly metabolize volatile fatty acids. The stimulation of microbial function increased the abundance of the methanogenesis pathways. Methanosarcina was the most dominant archaea in the four methanogenesis modules, and the microbial community changed more clearly with time than with treatment. This study is innovative in finding the effectiveness of BC and MB treatments attributed to the abundance of methanogenesis modules, especially MB2.5% treatment retained its advantage until the later stage of digestion in AD process.
The purpose of this study is to promote the efficient biogas production of food waste, and analyze the influence of adding biochar and magnetic biochar to anaerobic digestion characteristics about biogas production, process factors, and microbial and methanogenesis metabolic pathways. Study clarify the mechanism of two kinds biochar influence food waste anaerobic digestion: the effectiveness of biochar and magnetic biochar treatments attributed to the abundance of methanogenesis modules, especially magnetic biochar treatment retained it advantage until the later stage of digestion in AD process. This study is helpful to improve the theory of biochar stimulating anaerobic digestion and guide biogas production.
Psoriasis is an inflammatory skin disease that is intricately linked to oxidative stress. Antioxidation and inhibition of abnormal proliferation of keratinocytes are pivotal strategies for psoriasis. ...Delivering drugs with these effects to the site of skin lesions is a challenge that needs to be solved. Herein, we reported a nanotransdermal delivery system composed of all-trans retinoic acid (TRA), triphenylphosphine (TPP)-modified cerium oxide (CeO2) nanoparticles, flexible nanoliposomes and gels (TCeO2-TRA-FNL-Gel). The results revealed that TCeO2 synthesized by the anti-micelle method, with a size of approximately 5 nm, possessed excellent mitochondrial targeting ability and valence conversion capability related to scavenging reactive oxygen species (ROS). TCeO2-TRA-FNL prepared by the film dispersion method, with a size of approximately 70 nm, showed high drug encapsulation efficiency (>96%). TCeO2-TRA-FNL-Gel further showed sustained drug release behaviors, great transdermal permeation ability, and greater skin retention than the free TRA. The results of in vitro EGF-induced and H2O2-induced models suggested that TCeO2-TRA-FNL effectively reduced the level of inflammation and alleviated oxidative stress in HaCat cells. The results of in vivo imiquimod (IMQ)-induced model indicated that TCeO2-TRA-FNL-Gel could greatly alleviate the psoriasis symptoms. In summary, the transdermal drug delivery system designed in this study has shown excellent therapeutic effects on psoriasis and is prospective for the safe and accurate therapy of psoriasis.
Display omitted TCeO2 and TRA are encapsulated into flexible nanoliposomes (TCeO2-TRA-FNL), and then TCeO2-TRA-FNL is loaded into gel to be endowed the ability for better skin retention. TCeO2-TRA-FNL could penetrate the stratum corneum and enter keratinocytes. TRA would then be released to inhibit the abnormal proliferation of keratinocytes and inflammation, and TCeO2 would target mitochondria to scavenge excessive ROS.
SARS‐CoV‐2 aptamer is a favorable candidate for the recognition and detection of SARS‐CoV‐2, owing to its small size and easy synthesis. However, the issue of compromised binding affinities in real ...samples and targeting mutant SARS‐CoV‐2 hinder wide applications of the aptamer. In this study, it is discovered that molecular crowding could increase binding affinity of CoV2‐6C3 aptamer against RBD (Receptor Binding Domain) of SARS‐CoV‐2 via increasing the absolute value of the enthalpy change. The values of the equilibrium dissociation constant in molecular crowding decrease by 70% and 150%, respectively, against wild‐type and mutant RBD compared with those in buffer without crowding. Moreover, the detection limit of SARS‐CoV‐2 pseudovirus is up to 5 times lower under molecular crowding compared to dilute conditions. The discovery deepens the understanding of aptamer‐target interaction mechanisms in crowding conditions and provides an effective way to apply SARS‐CoV‐2 aptamer for virus recognition and detection.
The impact of changing meteorological conditions on crop quality has become a trending topic in current agriculture research. In this study, we analyzed the combined effects of both meteorological ...conditions and key stages on the nutritional quality of maize based on the data of field-staged sowing trials from 2018 to 2022. The results are as follows: (1) The key stage of meteorological conditions affecting the content of major nutritional qualities of maize is from 6 d before to 35 d after flowering. (2) The maximum temperature from 6 d before to 8 d after flowering, average temperature from 5 d before to 20 d after flowering, and minimum temperature from 9–20 d after flowering have significant positive effects on protein, fat, and essential amino acids, respectively; the daily difference in temperature from 9 to 35 d and 24 to 35 d after flowering have a significant negative effect on crude fiber and essential amino acids, respectively, and the daily difference in temperature from 24 to 35 d and the minimum temperature from 5 d before to 35 d after flowering have a significant effect on non-essential amino acids. (3) When the maximum temperature during the key stage of nutritional quality is 31.2 °C, the average temperatures are 24.9 °C and 22.4 °C, the minimum temperature is 18.9 °C, and the daily difference in temperature is 15.0 °C, the contents could reach the optimal values of 9.66% (protein), 4.80% (fat), 4.97% (crude fiber), 40.39 g·kg−1 (essential amino acids), and 58.96 g·kg−1 (non-essential amino acids), respectively. The findings provide a basis for adjusting the sowing period to improve the nutritional quality of maize in the context of climate change.
Gliosarcoma, a variant of glioblastoma multiforme (GBM), is a highly invasive malignant tumor. Unfortunately, this disease still marked by poor prognosis regardless of modern treatments. It is of ...great significance to discover specific molecular probes targeting gliosarcoma for early cancer diagnosis and therapy. Herein, we have selected a group of DNA aptamers with high affinity and selectivity against gliosarcoma cells K308 using cell-SELEX. All the dissociation constants of these aptamers against gliosarcoma cells were in the nanomolar range and aptamer WQY-9 has the highest affinity and good selectivity among them. Furthermore, truncated aptamer sequence, WQY-9-B, shows similar recognition ability to aptamer WQY-9. In addition, WQY-9-B was found to be able to bind selectively and internalize into cytoplasm of target cancer cell at 37°C. More importantly, compared to a random sequence, aptamer WQY-9-B showed excellent recognition rate (73.3%) for tissue sections of clinical gliosarcoma samples. These data suggests that aptamer WQY-9-B has excellent potential as an effective molecular probe for gliosarcoma diagnosis.
•We identified aptamers with high affinity specifically target gliosarcoma cell using whole cell SELEX.•Truncated aptamer WQY-9-B, can not only recognize gliosarcoma cell but also clinical gliosarcoma tissue.•Aptamer WQY-9-B can be internalized into gliosarcoma cell at 37°C which would be beneficial for potential target drug delivery and therapy.
Wound healing is an urgent clinical challenge, particularly in the case of chronic wounds. Traditional approaches to wound healing have limited therapeutic efficacy due to lengthy healing times, risk ...of immune rejection, and susceptibility to infection. Recently, adipose-derived mesenchymal stem cell-derived exosomes (ADSC-exos) have emerged as a promising modality for tissue regeneration and wound repair. In this study, the development of a novel extracellular matrix hydrogel@exosomes (ECM@exo) is reported, which entails incorporation of ADSC-exos into an extracellular matrix hydrogel (ECM hydrogel). This solution forms a hydrogel at physiological temperature (≈37 °C) upon local injection into the wound site. ECM@exo enables sustained release of ADSC-exos from the ECM hydrogel, which maintains high local concentrations at the wound site. The ECM hydrogel displays good biocompatibility and biodegradability. The in vivo and in vitro results demonstrate that ECM@exo treatment effectively reduces inflammation and promotes angiogenesis, collagen deposition, cell proliferation, and migration, thereby accelerating the wound healing process. Overall, this innovative therapeutic approach offers a new avenue for wound healing via a biological hydrogel with controlled exosome release.
Bi1−xNdxFe0.99Mn0.01O3 (BNFMO, x = 0.00~0.20) films were epitaxially grown on Nb:SrTiO3 (001) substrates using pulsed laser deposition. It was found that the Nd-doping concentration has a great ...impact on the surface morphology, crystal structure, and electrical properties. BNFMO thin film with low Nd-doping concentration (≤16%) crystallizes into a rhombohedral structure, while the high Nd-doping (>16%) will lead to the formation of an orthogonal structure. Furthermore, to eliminate the resistive switching (RS) effect, a positive-up–negative-down (PUND) measurement was applied on two devices in series. The remnant polarization experiences an increase with the Nd-doping concentration increasing to 16%, then drops down with the further increased concentration of Nd. Finally, the ferroelectric photovoltaic effect is also regulated by the ferroelectric polarization, and the maximum photocurrent of 1758 μA/cm2 was obtained in Bi0.84Nd0.16Fe0.99Mn0.01O3 thin film. BNFMO films show great potential for ferroelectric and photovoltaic applications.
The capability to design and modulate materials, shapes, heat transfer, and mass mixing during the process of developing chemical reactors has allowed researchers to explore millions of chemical ...reactions and assays. However, despite the advantages in engineering array-based microreactors or microfluidic systems, the wetting attachment between solutions of reagents/products and the glass or polymer substrates of containers leads to difficulties in collecting products effectively and preventing channel blockage. Herein we present a miniature droplet reactor which takes advantage of the anti-wetting and low-adhesive properties of nanoparticle-derived superhydrophobic pedestals, allowing aqueous droplets to be manipulated freely but also providing a confined environment for performing a series of aqueous phase chemical reactions on a small scale. Gas- or precipitate- forming reactions can also be performed inside this miniature reactor. Most importantly, reaction products in liquid, solid or gaseous states can be collected effectively, which allows the harvesting of valuable products formed in limited amounts. Such a miniature reactor built on superhydrophobic pedestals provides a new way of performing common chemical reactions and may open the door to the design of next-generation microreaction systems.
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