Circular RNAs (circRNAs), a novel class of noncoding RNAs, have recently drawn lots of attention in the pathogenesis of human cancers. However, the role of circRNAs in cancer cells ...epithelial-mesenchymal transition (EMT) remains unclear. In this study, we aimed to identify novel circRNAs that regulate urothelial carcinoma of the bladder (UCB) cells' EMT and explored their regulatory mechanisms and clinical significance in UCBs.
We first screened circRNA expression profiles using a circRNA microarray in paired UCB and normal tissues, and then studied the clinical significance of an upregulated circRNA, circPRMT5, in a large cohort of patients with UCB. We further investigated the functions and underlying mechanisms of circPRMT5 in UCB cells' EMT. Moreover, we evaluated the regulation effect of circPRMT5 on miR-30c, and its target genes,
and
, in two independent cohorts from our institute and The Cancer Genome Atlas (TCGA).
We demonstrated that upregulated expression of circPRMT5 was positively associated with advanced clinical stage and worse survival in patients with UCB. We further revealed that circPRMT5 promoted UCB cell's EMT via sponging miR-30c. Clinical analysis from two independent UCB cohorts showed that the circPRMT5/miR-30c/SNAIL1/E-cadherin pathway was essential in supporting UCB progression. Importantly, we identified that circPRMT5 was upregulated in serum and urine exosomes from patients with UCB, and significantly correlated with tumor metastasis.
CircPRMT5 exerts critical roles in promoting UCB cells' EMT and/or aggressiveness and is a prognostic biomarker of the disease, suggesting that circPRMT5 may serve as an exploitable therapeutic target for patients with UCB.
Engineering the adsorption of molecules on active sites is an integral and challenging part for the design of highly efficient transition‐metal‐based catalysts for methanol dehydrogenation. A ...Mott–Schottky catalyst composed of Ni nanoparticles and tailorable nitrogen‐doped carbon‐foam (Ni/NCF) and thus tunable adsorption energy is presented for highly efficient and selective dehydrogenation of gas‐phase methanol to hydrogen and CO even under relatively high weight hourly space velocities (WHSV). Both theoretical and experimental results reveal the key role of the rectifying contact at the Ni/NCF boundaries in tailoring the electron density of Ni species and enhancing the absorption energies of methanol molecules, which leads to a remarkably high turnover frequency (TOF) value (356 mol methanol mol−1 Ni h−1 at 350 °C), outpacing previously reported bench‐marked transition‐metal catalysts 10‐fold.
Active boundaries: The ability of Mott–Schottky‐type nanocatalysts to change the adsorption energy of methanol molecules for highly efficient and selective dehydrogenation of gas‐phase methanol was achieved by constructing a Ni nanoparticle/N‐doped carbon‐foam catalyst. The electron redistribution along the Ni‐N‐doped carbon phase boundaries promotes adsorption and activation of methanol, thus boosting methanol dehydrogenation.
In this letter, an alternating iterative adaptive approach (AIAA) for direction of arrival (DOA) estimation via acoustic vector sensor (AVS) array under directivity bias is proposed. Firstly, the ...directivity bias parameter is introduced into the signal model. An AIAA is then developed to estimate the sparse signal and the directivity bias matrix iteratively. In each iteration, in order to eliminate the effect of directivity bias, the directivity bias matrix reconstruction method based on the inherent directivity of AVS is presented. Simulation results show that the proposed method effectively improves the accuracy of DOA estimation compared with other state-of-the-art methods.
Fever is a common symptom of many infections, e.g., in the ongoing COVID-19 pandemic, keeping monitoring devices such as thermometers in constant demand. Recent technological advancements have made ...infrared (IR) thermometers the choice for contactless screening of multiple individuals. Yet, even so, the measurement accuracy of such thermometers is affected by many factors including the distance from the volunteers’ forehead, impurities (such as sweat), and the location measured on the volunteers’ forehead. To overcome these factors, we describe the assembly of an Arduino-based digital IR thermometer with distance correction using the MLX90614 IR thermometer and HC-SR04 ultrasonic sensors. Coupled with some analysis of these factors, we also found ways to programme compensation methods for the final assembled digital IR thermometer to provide more accurate readings and measurements.
Spinal long-term potentiation (LTP) at C-fiber synapses is hypothesized to underlie chronic pain. However, a causal link between spinal LTP and chronic pain is still lacking. Here, we report that ...high-frequency stimulation (HFS; 100 Hz, 10 V) of the mouse sciatic nerve reliably induces spinal LTP without causing nerve injury. LTP-inducible stimulation triggers chronic pain lasting for more than 35 days and increases the number of calcitonin gene-related peptide (CGRP) terminals in the spinal dorsal horn. The behavioral and morphological changes can be prevented by blocking NMDA receptors, ablating spinal microglia, or conditionally deleting microglial brain-derived neurotrophic factor (BDNF). HFS-induced spinal LTP, microglial activation, and upregulation of BDNF are inhibited by antibodies against colony-stimulating factor 1 (CSF-1). Together, our results show that microglial CSF1 and BDNF signaling are indispensable for spinal LTP and chronic pain. The microglia-dependent transition of synaptic potentiation to structural alterations in pain pathways may underlie pain chronicity.
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•HFS triggers synaptic plasticity of CGRP afferents and chronic pain•LTP-inducible HFS activates spinal microglia through CSF1 signaling•Microglial BDNF is essential for HFS-induced spinal LTP and chronic pain
Zhou et al. characterize chronic pain behaviors triggered by LTP-inducible HFS without nerve injury. They identify that HFS-induced LTP is accompanied by an increase in CGRP terminals in the spinal dorsal horn. Activation of neuronal CSF1-microglial BDNF signaling is indispensable for the synaptic and structural plasticity underlying HFS-induced chronic pain.
SUMMARY
Wurfbainia villosa is a well‐known medicinal and edible plant that is widely cultivated in the Lingnan region of China. Its dried fruits (called Fructus Amomi) are broadly used in traditional ...Chinese medicine for curing gastrointestinal diseases and are rich in volatile terpenoids. Here, we report a high‐quality chromosome‐level genome assembly of W. villosa with a total size of approximately 2.80 Gb, 42 588 protein‐coding genes, and a very high percentage of repetitive sequences (87.23%). Genome analysis showed that W. villosa likely experienced a recent whole‐genome duplication event prior to the W. villosa–Zingiber officinale divergence (approximately 11 million years ago), and a recent burst of long terminal repeat insertions afterward. The W. villosa genome enabled the identification of 17 genes involved in the terpenoid skeleton biosynthesis pathway and 66 terpene synthase (TPS) genes. We found that tandem duplication events have an important contribution to the expansion of WvTPSs, which likely drove the production of volatile terpenoids. In addition, functional characterization of 18 WvTPSs, focusing on the TPS‐a and TPS‐b subfamilies, showed that most of these WvTPSs are multi‐product TPS and are predominantly expressed in seeds. The present study provides insights into the genome evolution and the molecular basis of the volatile terpenoids diversity in W. villosa. The genome sequence also represents valuable resources for the functional gene research and molecular breeding of W. villosa.
Significance Statement
The present study reports a high‐quality chromosome‐level reference genome of Wurfbainia villosa with comprehensive genomic, transcriptomic, and metabolic analyses, as well as the identification and functional characterization of 66 terpene synthase (TPS)‐encoding genes, which can provide insights into the molecular genetic basis of the diversity and abundance of volatile terpenoids.
Carbon pricing is expected to meet COP26 targets and carbon neutrality goals by promoting energy transition. However, the role of carbon pricing in energy transition has been challenged largely ...because of limited empirical evidence. Most empirical studies draw on samples with low-to-moderate carbon prices, which might underestimate the potential impact of carbon pricing with much higher prices. Accordingly, this study takes energy prices as proxies for carbon prices to estimate the price elasticity of energy consumption. It employs a mapping relationship between carbon and energy prices to conduct simulations under various carbon price scenarios. Using China's industry as an example, the following main findings were obtained. First, the price elasticities of energy consumption are heterogeneous across sectors, implying that the role of carbon pricing may be underestimated if sector heterogeneity is not considered. Second, carbon pricing could considerably decrease energy consumption, particularly in high-energy-consuming sectors, such as ferrous metals, petroleum processing, chemicals, non-metal products, as well as power and heat. Third, by adopting China's Emissions Trading Scheme, the eight planned covered sectors could achieve reductions of 8% or 19% in energy consumption with carbon prices set at 100 or 300 Yuan/tCO2, respectively, which would significantly contribute to the phasedown of coal. Therefore, carbon pricing could play an important role in achieving energy transition in the post-COP26 era.
•The effectiveness of carbon pricing on energy transition in the post-COP26 era are studied.•Carbon pricing could generate a considerable decrease in energy consumption.•Heterogeneous price elasticities avoid underestimation of energy conservation.•China's ETS could reduce energy consumption by 19% with a carbon price of 300 Yuan/tCO2.•China's ETS would also significantly contribute to the phasedown of coal.
•Non pharmacological interventions are effective in treating METH use disorder.•Contingency management and cognitive behavioural therapy yield better outcomes.•Heterogeneity noted across the studies ...in terms of methodology and interpretation.•The methodology should be standardised to allow comparison across the studies.
Methamphetamine (METH) use is on the rise globally, with the number of treatment seekers increasing exponentially across the globe. Evidence-based therapies are needed to meet rising treatment needs. This systematic review intends to appraise the existing evidence to identify effective non-pharmaceutical approaches for the treatment of METH use disorder.
Five electronic bibliographic databases-Ovid (Medline), Embase, Cumulative Index of Nursing and Allied Health Literature (CINAHL), Web of Science and PsycINFO- were searched to identify relevant studies that were published between January 1995 to February 2020. Studies were selected and assessed by two independent reviewers. A systematic review of data from both randomised control trials (RCT) and non-RCTs was conducted to appraise the evidence.
A total of 44 studies were included in the review. Behavioural interventions, i.e. cognitive behavioural therapy (CBT), contingency management (CM), exercise, residential rehabilitation based therapies, repetitive transcranial magnetic stimulation (rTMS), and matrix model demonstrated treatment efficacy in promoting abstinence, reducing methamphetamine use or craving in the participants. While CM interventions showed the strongest evidence favouring the outcomes assessed, tailored CBT alone or with CM was also effective in the target population.
Behavioural interventions should be considered as the first line of treatment for methamphetamine use disorder. Future studies should address the longevity of the effects, and limitations due to smaller sample sizes and high dropout rates to enable better assessment of evidence.
Nasal polyps are a significantly associated pathology of chronic rhinosinusitis (CRS) whose mechanisms of pathogenesis are not fully elucidated, especially the interaction of the polyp with its ...environment that allows its growth on the nasal epithelial lining. Exosomes are nanovesicles that serve important biological functions, including cell-to-cell signaling and communication.
Hence, we sought to explore the roles of the epithelial-derived exosomal proteome obtained from the human nasal epithelium in the modulation of CRS with nasal polyp (CRSwNP) pathogenesis.
We sampled exosomes from nasal lavage fluid and primary human nasal epithelial cells (hNECs) from healthy controls and patients with CRSwNP with and without coexisting asthma. The presence of exosomes was confirmed using a NanoSight assay, transmission electron microscopy and western blotting. The exosomal proteome was profiled with mass spectrometry. The Cell Counting Kit-8 was used to confirm the roles of exosomes in mediating cellular proliferation.
The hNEC-derived exosomes from diseased epithelium contained differentially expressed proteins that were mainly involved in epithelial remodeling via pathways such as p53. An in vitro study further demonstrated that epithelial-derived exosomes from patients with CRSwNP (with and without coexisting asthma) significantly reduced the rate of proliferation of control hNECs at an effective concentration of ≥10 μg/ml.
Exosomes secreted by hNECs from patients with CRSwNP, regardless of their coexistence with asthma, are laden with proteins that influence cell proliferation pathways, potentially leading to remodeling of the sinonasal mucosa.
Diseased (CRSwNP with and without coexisting asthma) human nasal epithelial cells (hNECs) were found to communicate with the environment via exosomes. Both healthy and exosomal protein signatures include immune response cytokines. Diseased epithelium-derived exosomes secreted increased cell cycle signatures involved in such as p53 pathway that might contribute to the inhibition of healthy epithelial proliferation, potentially as a mechanism of remodeling. Display omitted
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