Abstract
Background
Nanosilver fluoride (NSF) was developed as an alternative in the prevention of dental caries.
Purpose
The aim of this study was to test the remineralizing action of NSF on ...incipient enamel caries and its antimicrobial action on the acid production and adhesion of
Streptococcus mutans.
Methods
Deciduous enamel fragments were treated with sodium fluoride (NaF), NSF and deionized water. Microhardness, fluorescence spectroscopy and optical coherence tomography imaging were performed on each specimen before chemical caries induction, after caries induction and after 14 days of pH cycling. The treated enamel fragments were also placed into test tubes containing bacterial suspension and saliva. The pH readings and quantification of the adhered microorganisms to the dental enamel were determined. Analysis of variance, Kruskal-Wallis, Mann-Whitney, Tukey and mixed linear regression model were applied.
Results
NSF and NaF were effective in enamel remineralization, with a statistically significant difference (p<0.001) to deionized water, and they had no statistically significant difference between themselves (p>0.005). NSF had greater effectiveness compared to NaF in preventing decreases of pH and adhesion of
S. mutans
to the enamel surface, with statistically significant (p<0.001) differences.
Conclusion
NSF may be more effective than conventional fluorides in treating incipient caries lesions due to its remineralization and antibacterial actions.
Silver nanoparticles stabilized with chitosan biopolymer are a new antibacterial agent for treatment of caries. To determine whether the size and morphology of silver nanoparticles in colloidal ...solution altered their antimicrobial activity, four colored colloids were characterized by ultraviolet–visible (UV–Vis) spectroscopy and transmission electron microscopy (TEM), and their antimicrobial activity minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against reference stocks of
Streptococcus mutans
ATCC 25175 compared with chlorhexidine and silver diamine fluoride as controls. TEM revealed that the seed solution (SS) contained only spherical nanoparticles with size of 8.7 ± 3.1 nm whereas the other colloids had predominantly spherical nanoparticle shape, although colloid 1 (S1) contained 2.5% triangular nanoparticles. Colloid 2 (S2) had 15.9% triangular and 6.8% cylindrical nanoparticles, while 23.3% of the silver nanoparticles in colloid 3 (S3) were triangular and 1.5% were cylindrical. All the silver nanoparticle colloids showed antimicrobial activity with no statistically significant differences (
p
> 0.05) in MIC or MBC. The colloids showed antimicrobial activity higher than silver diamine fluoride (
p
< 0.05) and equivalent to chlorhexidine (
p
> 0.05). The size and shape of the nanoparticles did not affect the antimicrobial properties of the colored colloids. Thus, the antimicrobial activity of the colloids containing silver nanoparticles against
Streptococcus mutans
was considered equivalent to that of chlorhexidine, the gold-standard antibiotic in dentistry.
An experimental dentifrice containing nano-silver fluoride (NSF) and a sodium fluoride (NaF) toothpaste were tested in vitro, against S. mutans, to evaluate the minimal inhibitory concentration ...(MIC), minimal bactericidal concentration (MBC), antiadherence, antiacid, enamel microhardness, and OCT. The microdilution technique was used to determine the MIC and MBC. Fragments of deciduous enamel were treated with dentifrice slurries, containing bacterial suspension and PBS-treated saliva. The quantification of the microorganisms that adhered to the enamel was determined after 24 hours of incubation, and media pH readings were performed after 2 hours and 24 hours. Deciduous teeth were evaluated for microhardness and OCT during 14 days of pH cycling. Data were statistically analyzed using Student’s t-test, Mann–Whitney U test, ANOVA, and Tukey tests at 5% of significance. Dentifrices containing NSF presented a lower MIC and higher statistically significant results compared to NaF dentifrices with respect to preventing bacterial adhesion and pH decreases. NSF and NaF dentifrices showed the same ability to avoid enamel demineralization corroborated by the OCT images. The NSF formulation had a better antibacterial effect compared to NaF dentifrices and similar action on the demineralization of enamel indicating their potential effectiveness to prevent caries.
A Odontologia hospitalar é a nova especialidade odontológica que atua na promoção da saúde bucal de indivíduos que necessitam de assistência em ambiente hospitalar, beneficiando a homeostase da saúde ...geral do paciente. O objetivo deste estudo foi discutir a importância do cirurgião-dentista na equipe multidisciplinar das unidades de terapia intensiva (UTI) através de um estudo transversal e qualitativo, do tipo survey, que utilizou como instrumento de pesquisa um questionário aplicado numa amostra de 15 cirurgiões-dentistas atuantes na UTI de hospitais públicos e privados de Pernambuco. Os dados obtidos foram submetidos à análise estatística descritiva. Quanto à atuação do profissional na UTI, cerca de 93,3% dos participantes consideraram comum a presença de lesões bucais no paciente internado e 66,7% classificaram o perfil bucal dos pacientes como sendo desfavorável. Sobre a presença do CD no âmbito hospitalar, a maior parte dos profissionais (73,3%) afirmou que a profissão ainda não tem o devido reconhecimento. Os dados obtidos nesse estudo confirmaram a importância da atuação do CD na prevenção e promoção de saúde dos pacientes internados em UTI e evidenciaram a falta de reconhecimento da presença desses profissionais nos hospitais.
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