Transplantation in mesangiocapillary glomerulonephritis with intramembranous dense “deposits”: Recurrence of disease. Six patients with mesangiocapillary glomerulonephritis and intramembranous dense ...“deposits” developed terminal renal failure and were transplanted, three from living and three from cadaver donors. Eight renal biopsy specimens were obtained from five of the grafts, from 1 to 26 months following transplantation. All six biopsy specimens taken later than seven months following the graft showed recurrence of dense intramembranous “deposits” in the basement membranes of glomeruli, and of Bowman's capsule and tubular basement membrane in five. Recurrence of “deposits” was associated with deposition of C3 on immunofluorescent study in all but one specimen; in addition, lgM was found in two specimens, but lgG and early complement components were absent. Only two patients, however, showed glomerular proliferation associated with profuse proteinuria. In the other subjects the recurrence of the “deposit” was not associated with clinical findings. Graft loss, which occurred in two patients, was predominantly from rejection.
Transplantation dans la glomerulopathie à dépôts denses intra-membraneux: Récidive de la maladie. Six malades atteints de glomérulopathie à dépôts denses intra-membraneux ont atteint le stade d'insuffisance rénale terminale et ont été transplantés. Trois d'entre eux ont reçu un rein de donneur vivant et les trois autres un rein de cadavre. Huit biopsies rénales ont été réalisées sur cinq de ces transplants un à trente-six mois après la transplantation. Les six biopsies prélevées après le septième mois de la transplantation montrent toutes la récidive des dépôts denses intra-membraneux dans les glomérules et cinq d'entre elles montrent aussi ces dépôts dans la capsule de Bowman et la membrane basale tubulaire. La récidive des dépôts est associée à la présence de C3, révélée par l'étude en immunofluorescence, dans toutes les biopsies sauf une. De plus, de l'IgM a été trouvée dans deux biopsies mais l'IgG et les facteurs précoces du complément sont absents. Deux malades seulement ont une prolifération glomérulaire associée à une protéinurie importante. Chez les autres, la récidive des dépôts n'est pas contemporaine de manifestations cliniques. La destruction du greffon, qui est survenue chez deux malades, a été essentiellement le fait du rejet.
Treatment of cell suspension cultures of French bean (Phaseolus vulgaris) with polysaccharide elicitor molecules from cell walls of the anthracnose fungus, Colletotrichum lindemuthianum, results in ...the rapid accumulation of isoflavonoid phytoalexins, deposition of wall-bound phenolic compounds and synthesis of hydroxyproline-rich glycoproteins. These changes are dependent upon a highly selective induction of gene products, including the enzymes L-phenylalanine ammonia-lyase, cytochrome P450-dependent cinnamic acid 4-hydroxylase, chalcone synthase, chalcone isomerase, prolyl hydroxylase and protein:arabinosyl transferase. Use of in vivo labelling, in vitro translation and RNA blot hybridization techniques has shown that these elicitormediated changes arise from rapid but transient induction of enzyme synthesis, resulting from the accumulation of specific mRNAs. Similar phenomena are observed in bean hypocotyls at the onset of phytoalexin synthesis in response to infection by incompatible and compatible races of C. lindemuthianum. In bean, both L-phenylalanine ammonia-lyase and chalcone synthase are encoded by multigene families and, at the protein level, both exhibit subunit and intact enzyme polymorphism. A number of less than full-length phenylalanine ammonialyase copy DNAs containing identical open reading frames have been produced from mRNA from elicitor-induced bean cells. Analysis of phenylalanine ammonia-lyase genomic clones predicts the presence of enzyme forms of differing amino acid sequence. In cultured bean cells, elicitor differentially induces the two apparent phenylalanine ammonia-lyase iso-forms with the lowest Kmvalues. In addition to transcriptional control of the appearance of specific gene products, post-translational processes may result in increased subunit polymorphism for phenylalanine ammonia-lyase, and in the activation of chalcone isomerase. Changes in endogenous phenylpropanoid intermediate pools may signal the rapid removal of phenylalanine ammonia-lyase activity, in addition to exerting less specific inhibitory effects on the formation and/or activity of the mRNAs encoding phenylalanine ammonia-lyase and other phytoalexin biosynthetic enzymes.
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