Human epidermal growth factor receptor 2 (HER2) has been identified as a molecular target for breast cancer therapy, such as Trastuzumab (Herceptin®). This has been shown to improve patient survival ...substantially. The current study is aiming to locally produce an anti-HER2 monoclonal antibody (named Trastuzumab) which has an equivalent biological properties in comparison with the original version, Herceptin®). In silico design and construction of recombinant vectors, as well as the establishment of transfected cell lines with high expression of Trastuzumab were performed. Based on the protein sequences obtained from the Drugbank, the DNA sequences encoding for the light chain (Tras-Lc) and heavy chain (Tras-Hc) of Trastuzumab were optimized and integrated into pNanogen-Hygro and pNanogen-Puro vectors, respectively. The Neon Transfection System was used to co-transfect the pNanogen-Tras-Lc-Hygro and pNanogen-Tras-Hc-Puro constructs into CHO cells. Different co-transfected single-cell-colonies selected on media supplemented with hygromycin and puromycin were used for ELISA and SDS-PAGE assays to identify the CHO cell lines which highly express Trastuzumab. Based on the present results, 30μg of both constructs were suitable for DNA co-transfection. After 07 days of culture, the highest amount of Trastuzumab (561 µg/ml) was obtained from the H06LD68 cell line.
Smart homes are an element of developing smart cities. In recent years, countries around the world have spared no effort in promoting smart cities. Smart homes are an interesting technological ...advancement that can make people’s lives much more convenient. The development of smart homes involves multiple technological aspects, which include big data, mobile networks, cloud computing, Internet of Things, and even artificial intelligence. Digital information is the main component of signal control and flow in a smart home, while information security is another important aspect. In the event of equipment failure, the task of safeguarding the system’s information is of the utmost importance. Since smart homes are automatically controlled, the problem of mobile network security must be taken seriously. To address these issues, this paper focuses on information security, big data, mobile networks, cloud computing, and the Internet of Things. Security efficiency can be enhanced by using a Secure Hash Algorithm 256 (SHA-256), which is an authentication mechanism that, with the help of the user, can authenticate each interaction of a given device with a WebServer by using an encrypted username, password, and token. This framework could be used for an automated burglar alarm system, guest attendance monitoring, and light switches, all of which are easily integrated with any smart city base. In this way, IoT solutions can allow real-time monitoring and connection with central systems for automated burglar alarms. The monitoring framework is developed on the strength of the web application to obtain real-time display, storage, and warning functions for local or remote monitoring control. The monitoring system is stable and reliable when applying SHA-256.
In this study, valuable polyhydroxynaphthoquinone (PHNQ) pigments were recovered from sea urchin food waste and were investigated as a potential bioactive ingredient for cosmeceuticals. The crude ...PHNQ pigment extract from 4 Vietnam sea urchins, Diadema setosum, Diadema savignyi, Stomopneustes variolaris, and Tripneustes gratilla, exhibited effective 2,2-diphenyl-1-picryl-hydrazyl-hydrate scavenging activity, tyrosinase inhibitory activity, and antibacterial activity. The moisturizing cream with 0.5% of PHNQ pigments from D. setosum and Tripneustes gratilla sea urchins showed no dermal irritation over 14 days of mouse skin test. Four major active components in PHNQ were identified via high-performance liquid chromatography coupled with diode array detector and mass spectrometry. Echinochrome A contributed considerably to the antioxidant activity of the extracts while those containing echinochrome A and spinochrome E were significantly active against various bacteria. The promising results laid the foundation for establishing a novel process from food waste to innovative biomaterial and formulating eco-friendly skincare products with PHNQ components from sea urchins as precious ingredient.
Spatial repellency of metofluthrin-impregnated polyethylene latticework plastic strips against Aedes aegypti mosquitoes was evaluated. Analysis of environmental factors affecting the efficacy of ...these strips, such as room temperature, humidity, and house structure, was performed in a residential area in My Tho City, Tien Giang Province, Vietnam. Treatment with the strips at the rate of 1 strip per 2.6-5.52 m(2) (approximately 600 mg per 2.6-5.52 m(2)) reduced the collection of Ae. aegypti resting inside the houses for at least eight weeks. Multiple regression analysis indicated that both increase in the average room temperature and decrease in the area of openings in the rooms that were treated with the strips positively affected the spatial repellency of metofluthrin.
By using near-infrared fluorescent protein (iRFP)-expressing hematopoietic cells, we established a novel, quantitative, in vivo, noninvasive atherosclerosis imaging system. This murine ...atherosclerosis imaging approach targets macrophages expressing iRFP in plaques. Low-density lipoprotein receptor-deficient (LDLR
) mice transplanted with beta-actin promoter-derived iRFP transgenic (TG) mouse bone marrow (BM) cells (iRFP → LDLR
) were used. Atherosclerosis was induced by a nonfluorescent 1.25% cholesterol diet (HCD). Atherosclerosis was compared among the three differently induced mouse groups. iRFP → LDLR
mice fed a normal diet (ND) and LDLR
mice transplanted with wild-type (WT) BM cells were used as controls. The in vivo imaging system (IVIS) detected an enhanced iRFP signal in the thoracic aorta of HCD-fed iRFP → LDLR
mice, whereas iRFP signals were not observed in the control mice. Time-course imaging showed a gradual increase in the signal area, which was correlated with atherosclerotic plaque progression. Oil red O (ORO) staining of aortas and histological analysis of plaques confirmed that the detected signal was strictly emitted from plaque-positive areas of the aorta. Our new murine atherosclerosis imaging system can noninvasively image atherosclerotic plaques in the aorta and generate longitudinal data, validating the ability of the system to monitor lesion progression.
Ulvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed Ulva papenfussii. U. papenfussii is an unexplored ...Ulva species found in the South China Sea along the central coast of Vietnam. Based on dry weight, the ulvan yield was ~15% (w/w) and the ulvan had a sulfate content of 13.4 wt%. The compositional constitution encompassed L-Rhamnose (Rhap), D-Xylose (Xylp), D-Glucuronic acid (GlcAp), L-Iduronic acid (IdoAp), D-Galactose (Galp), and D-Glucose (Glcp) with a molar ratio of 1:0.19:0.35:0.52:0.05:0.11, respectively. The structure of ulvan was determined using High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance spectroscopy (NMR) methods. The results showed that the extracted ulvan comprised a mixture of two different structural forms, namely (“A3s”) with the repeating disaccharide →4)-β-D-GlcAp-(1→4)-α-L-Rhap 3S-(1→n, and (“B3s”) with the repeating disaccharide →4)-α-L-IdoAp-(1→4)-α-L-Rhap 3S(1→n. The relative abundance of A3s, and B3s was 1:1.5, respectively. The potential anticarcinogenic attributes of ulvan were evaluated against a trilogy of human cancer cell lineages. Concomitantly, Quantitative Structure–Activity Relationship (QSAR) modeling was also conducted to predict potential adverse reactions stemming from pharmacological interactions. The ulvan showed significant antitumor growth activity against hepatocellular carcinoma (ICsub.50 ≈ 90 µg/mL), human breast cancer cells (ICsub.50 ≈ 85 µg/mL), and cervical cancer cells (ICsub.50 ≈ 67 µg/mL). The QSAR models demonstrated acceptable predictive power, and seven toxicity indications confirmed the safety of ulvan, warranting its candidacy for further in vivo testing and applications as a biologically active pharmaceutical source for human disease treatment.
In this paper, the dispersion of Ag metal nanoparticles on SBA-15 mesoporous silica and its catalytic performance to oxidize glucose to gluconic acid were studied. Mesoporous silica SBA-15 materials ...were synthesized using the triblock copolymer Pluronic P123 as a template in acid condition. Ag nanoparticles were prepared using impregnation method inside the pores of the support by controlled reduction of AgNO3 with sodium borohydrate (NaBH4). The XRD, TEM, EDS, BET techniques were used for characterization of materials. Silver nanoparticle formation is confirmed by TEM. The efficiency of glucose oxidation to gluconic acid is determinated by HPLC-RID and LC-MS. Obtained results showed that it can prepare silver nanopartilces with particle diameter about 5 nm; and catalyst based on these particles has quite efficiency in glucose oxidation to gluconic acid. DOI: 10.1380/ejssnt.2012.273
MafB, a transcription factor expressed selectively in macrophages, has important roles in some macrophage‐related diseases, especially in atherosclerosis. In this study, we investigated the mechanism ...by which hematopoietic‐specific MafB deficiency induces the development of obesity. Wild‐type and hematopoietic cell‐specific Mafb‐deficient mice were fed a high‐fat diet for 10 weeks. The Mafb‐deficient mice exhibited higher body weights and faster rates of body weight increase than control mice. The Mafb‐deficient mice also had a higher percentage of body fat than the wild‐type mice, due to increased adipocyte size and serum cholesterol levels. Reverse transcription‐PCR analysis showed a reduction in apoptosis inhibitor of macrophage (AIM) in Mafb‐deficient adipose tissue. AIM is known as an inhibitor of lipogenesis in adipocytes and is expressed in adipose tissue macrophages. Collectively, our data suggest that Mafb deficiency in hematopoietic cells accelerates the development of obesity.
We investigated whether hematopoietic cell‐specific Mafb deficiency induces obesity. Mafb‐deficient mice exhibited higher weights and higher body fat percentages with larger adipocytes, and higher levels of serum cholesterol. In Mafb‐deficient adipose tissue macrophages, the expression of AIM, the inhibitor for lipogenesis in adipocytes, was decreased.
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