Abiotic stress is one of the severe stresses of environment that lowers the growth and yield of any crop even on irrigated land throughout the world. A major phytohormone abscisic acid (ABA) plays an ...essential part in acting toward varied range of stresses like heavy metal stress, drought, thermal or heat stress, high level of salinity, low temperature, and radiation stress. Its role is also elaborated in various developmental processes including seed germination, seed dormancy, and closure of stomata. ABA acts by modifying the expression level of gene and subsequent analysis of
- and
-acting regulatory elements of responsive promoters. It also interacts with the signaling molecules of processes involved in stress response and development of seeds. On the whole, the stress to a plant can be susceptible or tolerant by taking into account the coordinated activities of various stress-responsive genes. Numbers of transcription factor are involved in regulating the expression of ABA responsive genes by acting together with their respective
-acting elements. Hence, for improvement in stress-tolerance capacity of plants, it is necessary to understand the mechanism behind it. On this ground, this article enlightens the importance and role of ABA signaling with regard to various stresses as well as regulation of ABA biosynthetic pathway along with the transcription factors for stress tolerance.
Background & objectives: The growing incidence and the wide diversity of carbapenemase-producing bacterial strains is a major concern as only a few antimicrobial agents are active on ...carbapenem-resistant bacteria. This study was designed to study molecular epidemiology of carbapenem-resistant Gram-negative bacterial (GNB) isolates from the community and hospital settings.
Methods: In this study, non-duplicate GNB were isolated from clinical specimens, and phenotypic test such as modified Hodge test, metallo β-lactamase E-strip test, etc. were performed on carbapenem-resistant bacteria. Multiplex PCR was performed to identify the presence of blaIMP, blaVIM, blaKPC, blaOXA48, blaOXA23, blaSPM, blaGIM, blaSIM and blaNDM. Minimum inhibitory concentration (MIC) of colistin, fosfomycin, minocycline, chloramphenicol and tigecycline was also determined.
Results: Of the 3414 GNB studied, carbapenem resistance was 9.20 per cent and maximum resistance (11.2%) was present at tertiary care centre, followed by secondary care (4%) and primary centre (2.1%). Among the carbapenem-resistant bacteria, overall, the most common isolate was Pseudomonas aeruginosa (24%). On multiplex PCR 90.3 per cent carbapenem-resistant isolates were positive for carbapenemase gene. The blaNDM(63%) was the most prevalent gene followed by blaVIM(18.4%). MIC results showed that 88 per cent carbapenem-resistant Enterobacteriaceae were sensitive to fosfomycin, whereas 78 per cent of P. aeruginosa and 85 per cent Acinetobacter spp. were sensitive to colistin.
Interpretation & conclusions: Carbapenem resistance in GNB isolates from the community and hospital settings was found to be on the rise and should be closely monitored. In the absence of new antibiotics in pipeline and limited therapeutic options, prudent use of antibiotics and strict infection control practices should be followed in hospital to limit the emergence and spread of multidrug-resistant bacteria.
Nanotechnology is a cutting-edge field of science with the potential to revolutionize today's technological advances including industrial applications. It is being utilized for the welfare of ...mankind; but at the same time, the unprecedented use and uncontrolled release of nanomaterials into the environment poses enormous threat to living organisms. Silver nanoparticles (AgNPs) are used in several industries and its continuous release may hamper many physiological and biochemical processes in the living organisms including autotrophs and heterotrophs. The present review gives a concentric know-how of the effects of AgNPs on the lower and higher autotrophic plants as well as on heterotrophic microbes so as to have better understanding of the differences in effects among these two groups. It also focuses on the mechanism of uptake, translocation, accumulation in the plants and microbes, and resulting toxicity as well as tolerance mechanisms by which these microorganisms are able to survive and reduce the effects of AgNPs. This review differentiates the impact of silver nanoparticles at various levels between autotrophs and heterotrophs and signifies the prevailing tolerance mechanisms. With this background, a comprehensive idea can be made with respect to the influence of AgNPs on lower and higher autotrophic plants together with heterotrophic microbes and new insights can be generated for the researchers to understand the toxicity and tolerance mechanisms of AgNPs in plants and microbes.
Recently, bioMérieux, France, introduced the Rapidec Carba NP test kit for rapid detection of carbapenemase-producing Gram-negative bacteria. This kit was evaluated in this study, and we report ...sensitivity, specificity, and positive and negative predictive values of 92.6%, 96.2%, 95.83%, and 92.6%, respectively. The test was easy to perform and interpret and relatively inexpensive ($5/Rs 300 per test) and provides a practical solution for early detection of carbapenemase-producing, multidrug-resistant Gram-negative bacteria.
The aqueous extract of leaf (LE), fruit (FE) and seed (SE) of Moringa oleifera was assessed to examine the ability to inhibit the oxidative DNA damage, antioxidant and anti-quorum sensing (QS) ...potentials. It was found that these extracts could significantly inhibit the OH-dependent damage of pUC18 plasmid DNA and also inhibit synergistically with trolox, with an activity sequence of LE>FE>SE. HPLC and MS/MS analysis was carried out, which showed the presence of gallic acid, chlorogenic acid, ellagic acid, ferulic acid, kaempferol, quercetin and vanillin. The LE was with comparatively higher total phenolics content (105.04mg gallic acid equivalents (GAE)/g), total flavonoids content (31.28mg quercetin equivalents (QE)/g), and ascorbic acid content (106.95mg/100g) and showed better antioxidant activity (85.77%), anti-radical power (74.3), reducing power (1.1 ascorbic acid equivalents (ASE)/ml), inhibition of lipid peroxidation, protein oxidation, OH-induced deoxyribose degradation, and scavenging power of superoxide anion and nitric oxide radicals than did the FE, SE and standard α-tocopherol. Eventually, LE and FE were found to inhibit violacein production, a QS-regulated behavior in Chromobacterium violaceum 12472.
•Quantifying geometric characteristics of 1600 street canyons in Geneva.•Solar access calculated for street-canyon surfaces and building facades.•Solar access in canyons depends strongly on ...street/facade orientations.•City scale effects of design parameters on solar access to the street canyon.
Buildings commonly have the largest share in the energy demand of a country, but they also offer sites for the generation of solar energy. Here we develop a methodology to analyse the effects of street-canyon geometries on the solar access of street surfaces and facades of the adjacent buildings at a city scale, using the city of Geneva (Switzerland) as a case study. In particular, we measured the following geometric parameters of 1600 street canyons: orientation, width, length, sky-view factor (SVF), and asymmetric aspect ratio. Street orientation has strong effect on received annual solar radiation by street surfaces and facades. For surfaces the highest received radiation (1000 kWh m−2) is for streets oriented WNE-ESE, whereas the highest radiation for facades (1400 kWh m−2) is for those facing SSW. The maximum monthly radiation received by street surfaces is 80 kWh m−2 whereas that received by facades is 100 kWh m−2. These maximum values are reached in June and July, but surfaces receive less radiation in all the months (the difference is mostly about 20 kWh m−2). Received solar radiation, both for street surfaces and facades, shows only moderate correlations with the other measured geometric parameters, namely street width, street length, asymmetric aspect ratio, and SVF, the highest coefficient of determination (R2 = 0.55) being between received street-surface radiation and SVF. Also, street surfaces receive the highest radiation when the aspect ratio is low or the SVF high. For a street surface to receive comparatively high radiation in the months May to August, the street needs to be more than 15 m wide, have an aspect ratio of less than 2.0, and a SVF above 0.1. The results for facades in the same months are generally similar, except that they receive much more radiation than the street surfaces. A city-scale design that minimises solar access of street surfaces during summers and maximises solar access of building facades during winters contributes to thermal comfort and may be partly reached through optimisation of urban density.
Growth and performance of various sectors of a country largely depends on the infrastructure developed by the country. It is true about the agricultural sector also. Along with the numbers, the ...location of these infrastructure play a vital role in reducing cost to the farmers while utilizing the existing resources like land, water, electricity and road network to their optimum level. India is the second largest producer of fruits, vegetable and tea. Against the total production of more than 500 MMT of horticultural & non-horticultural produces, the total cold storage capacity is estimated at approximately 31 MMT, which is not even 10% of the total production. In view of this, a site suitability study was conducted to develop an optimum plan to locate cold storages in the state of Uttar Pradesh, a leading potato growing state. Factors considered for evolving the plan includes the site of production, i.e. region where potato is grown, road network, existing cold storage, electricity and land use. When there are various factors behind any decision-making process, it becomes a complex process. It becomes difficult to decide which factor is to be given more importance and how other factors can be compounded with each other to arrive at a decision. Analytical Hierarchy process (AHP), helps us in determining the relative importance of various factors to arrive at a decision. In view of infrastructure planning, an illustrative map indicating spatial features is vital. As is widely established, remote sensing and GIS helps to obtain such a spatial map. Remote sensing and GIS was used to generate maps of the factors involved. AHP was used to develop weights of the factors in relation to each other to generate a map showing site suitability of places for developing cold storage facilities.
Different tillage methods i.e. zero tillage, natural farming, reduced tillage and conventional tillage in combination with 3 rice varieties, viz. Sukara Dhan 1 (HPR 1156), Him Palam Dhan 1 (HPR 2656) ...and Him Palam Lal Dhan 1 (HPR 2795) were evaluated in terms of growth, yield attributes and yield of direct seeded rice at research farm of Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Himachal Pradesh during rainy (kharif) season of 2020 and 2021. The experiment, was laid out in split plot design with tillage options in main plot and cultivars in subplot and replicated thrice. Findings exhibits that the, conventional tillage recorded significantly higher grain, straw and biological yield followed by reduced tillage and zero tillage while significantly lower values of all these traits were observed in natural farming during both the years of experimentation. Significantly greater and lower yields recorded in conventional tillage and natural farming treatments were due to the significantly higher and lower values of different yield attributes including number of panicles/m2, number of grains/panicle, grain weight/panicle and 1000-grain weight, observed in the respective treatments. From the present investigation it can be concluded that conventional tillage resulted in higher yield attributes and yield as compared to natural farming while lower values of yield attributes and yield were recorded under natural farming. Among different rice cultivars Him Palam Lal Dhan 1 was found superior than Sukara Dhan 1 and Him Palam Dhan 1.
In order to determine antioxidant activity, the five extracts/fractions of red onion peel were studied for their total content of phenolics (TPC), flavonoids (TFC), antioxidant activity (AOA), free ...radical scavenging activity (FRSA), assayed by DPPH radical in the terms of anti-radical power (ARP) and reducing power (RP), expressed as ascorbic acid equivalents (ASE)/ml. High TPC (384.7±5.0mg GAE/g), TFC (165.2±3.2mg QE/g), AOA (97.4±7.6%), ARP (75.3±4.5) and RP (1.6±0.3 ASE/ml) were found for the ethyl acetate (EA) fraction. EA fraction had markedly higher antioxidant capacity than butylated hydroxytoluene (BHT) in preventive or scavenging capacities against FeCl3-induced lipid peroxidation, protein fragmentation, hydroxyl (site-specific and non-site-specific), superoxide anion and nitric oxide radicals. EA fraction also showed dose dependent antimutagenic activity by following the inhibition of tobacco-induced mutagenicity in Salmonella typhimurium strains (TA102) and hydroxyl radical-induced nicking in plasmid pUC18 DNA. HPLC and MS/MS analysis showed the presence of ferulic, gallic, protocatechuic acids, quercetin and kaempferol. The large amount of polyphenols contained in EA fraction may cause its strong antioxidant and antimutagenic properties. This information shows that EA fraction of red onion peel can be used as natural antioxidant in nutraceutical preparations.
PDF
