•Influence of KLa on xylanase and β-xylosidase productions by Aspergillus niger in STB and ALB.•Batch fermentations in STB and ALB under a range of kLa for xylanases production.•Comparison of ...fermentations in STB and ALB for xylanolytic enzymes production.
Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000Ul−1; kLa=30h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000Ul−1 xylanase; 2100Ul−1 β-xylosidase) than STB (4500Ul−1 xylanase; 1800Ul−1 β-xylosidase).
ABSTRACT Objective: To assess the epidemiological profile of Jiu-Jitsu black belt athletes, including the prevalence of pain and shoulder function. Methods: Cross-sectional study carried out with ...Jiu-Jitsu athletes from 2014 to 2016. The studied variables were: sex, age, dominant limb, weight, height, profession, time of Jiu-Jitsu practice, weekly training hours, other practiced sports, comorbidities, injuries and previous surgeries, medications and habits. For the functional assessment of the shoulder, the ASES Score was used. Results: 53 male athletes were evaluated. There was a prevalence of alcohol consumption (60.4%) and supplement use (32.1%). The practice of other sports included weight training (49.1%) and other martial arts (17%). There was a prevalence of knee (66.0%) and shoulder (52.8%) injuries and, in some cases, the need for surgical procedures. There was a prevalence of shoulder pain (73.6%) and more than half of the athletes (52.9%) had minimal or moderate limitation of shoulder function. Conclusion: Jiu-jitsu black belt athletes often have a history of injuries, with the shoulder being the second most affected body part. In more than half of the athletes, there was a prevalence of shoulder pain and functional limitation, according to the ASES Score. Level of evidence III, Retrospective comparative study.
RESUMO Objetivo: Avaliar o perfil epidemiológico de atletas faixas-pretas de jiu-jitsu, incluindo a prevalência de dor e a função do ombro. Métodos: Estudo transversal realizado com atletas de jiu-jitsu entre 2014 e 2016. As variáveis estudadas foram: sexo, idade, membro dominante, peso, altura, profissão, tempo de prática do esporte, horas semanais de treino, outros esportes praticados, comorbidades, lesões e cirurgias prévias, medicamentos e hábitos. Para a avaliação funcional do ombro, foi utilizado o escore American Shoulder and Elbow Surgeons Standardized Shoulder Assessment Form (ASES). Resultados: Foram avaliados 53 atletas, todos do sexo masculino. Houve alta prevalência de consumo de bebida alcoólica (60,4%) e uso de suplementos (32,1%). A prática de outros esportes incluiu musculação (49,1%) e outras artes marciais (17%). Houve alta prevalência de lesões no joelho (66,0%) e no ombro (52,8%), e em alguns casos houve a necessidade de procedimentos cirúrgicos. Verificou-se alta prevalência de dor no ombro (73,6%), sendo que mais da metade dos atletas (52,9%) apresentaram limitação mínima ou moderada da função do ombro. Conclusão: Atletas faixas-pretas de jiu-jitsu frequentemente apresentam histórico de lesões, sendo o ombro o segundo local mais acometido. Houve alta prevalência de dor nos ombros e limitação funcional conforme o escore ASES em mais da metade dos atletas. Nível de evidência III, Estudo comparativo retrospectivo.
Interest in renewable energy sources has increased in recent years due to environmental concerns about global warming and air pollution,reduced costs and improved efficiency of technologies.Under the ...European Union（EU）energy directive,biomass is a suitable renewable source.The aim of this study was to experimentally quantify and characterize the emission of particulate matter（PM（2.5））resulting from the combustion of two biomass fuels（chipped residual biomass from pine and eucalypt）,in a pilot-scale bubbling fluidized bed（BFB）combustor under distinct operating conditions.The variables evaluated were the stoichiometry and,in the case of eucalypt,the leaching of the fuel.The CO and PM（2.5）emission factors were lower when the stoichiometry used in the experiments was higher（0.33±0.1 g CO/kg and 16.8±1.0 mg PM（2.5）/kg,dry gases）.The treatment of the fuel by leaching before its combustion has shown to promote higher PM（2.5）emissions（55.2±2.5 mg/kg,as burned）.Organic and elemental carbon represented 3.1 to 30 wt.% of the particle mass,while carbonate（CO3~（2-））accounted for between 2.3 and 8.5 wt.%.The particulate mass was mainly composed of inorganic matter（71% to 86% of the PM（2.5）mass）.Compared to residential stoves,BFB combustion generated very high mass fractions of inorganic elements.Chloride was the water soluble ion in higher concentration in the PM（2.5）emitted by the combustion of eucalypt,while calcium was the dominant water soluble ion in the case of pine.
A key event in atherogenesis is the formation of lipid‐loaded macrophages, lipidotic cells, which exhibit irreversible accumulation of undigested modified low‐density lipoproteins (LDL) in lysosomes. ...This event culminates in the loss of cell homeostasis, inflammation, and cell death. Nevertheless, the exact chemical etiology of atherogenesis and the molecular and cellular mechanisms responsible for the impairment of lysosome function in plaque macrophages are still unknown. Here, we demonstrate that macrophages exposed to cholesteryl hemiazelate (ChA), one of the most prevalent products of LDL‐derived cholesteryl ester oxidation, exhibit enlarged peripheral dysfunctional lysosomes full of undigested ChA and neutral lipids. Both lysosome area and accumulation of neutral lipids are partially irreversible. Interestingly, the dysfunctional peripheral lysosomes are more prone to fuse with the plasma membrane, secreting their undigested luminal content into the extracellular milieu with potential consequences for the pathology. We further demonstrate that this phenotype is mechanistically linked to the nuclear translocation of the MiT/TFE family of transcription factors. The induction of lysosome biogenesis by ChA appears to partially protect macrophages from lipid‐induced cytotoxicity. In sum, our data show that ChA is involved in the etiology of lysosome dysfunction and promotes the exocytosis of these organelles. This latter event is a new mechanism that may be important in the pathogenesis of atherosclerosis.
We show that cholesteryl hemiazelate, an end‐product of LDL‐derived cholesteryl ester oxidation, is involved in the etiology of lysosome dysfunction in atherosclerotic macrophages and promotes the exocytosis of these dysfunctional organelles. We further demonstrate that this phenotype is mechanistically linked to the nuclear translocation of the MiT/TFE family of transcription factors that protects macrophages from lipid‐induced cytotoxicity.
The goal of this trial was to study the long-term effects of intravenous (IV) metoprolol administration before reperfusion on left ventricular (LV) function and clinical events.
Early IV metoprolol ...during ST-segment elevation myocardial infarction (STEMI) has been shown to reduce infarct size when used in conjunction with primary percutaneous coronary intervention (pPCI).
The METOCARD-CNIC (Effect of Metoprolol in Cardioprotection During an Acute Myocardial Infarction) trial recruited 270 patients with Killip class ≤II anterior STEMI presenting early after symptom onset (<6 h) and randomized them to pre-reperfusion IV metoprolol or control group. Long-term magnetic resonance imaging (MRI) was performed on 202 patients (101 per group) 6 months after STEMI. Patients had a minimal 12-month clinical follow-up.
Left ventricular ejection fraction (LVEF) at the 6 months MRI was higher after IV metoprolol (48.7 ± 9.9% vs. 45.0 ± 11.7% in control subjects; adjusted treatment effect 3.49%; 95% confidence interval CI: 0.44% to 6.55%; p = 0.025). The occurrence of severely depressed LVEF (≤35%) at 6 months was significantly lower in patients treated with IV metoprolol (11% vs. 27%, p = 0.006). The proportion of patients fulfilling Class I indications for an implantable cardioverter-defibrillator (ICD) was significantly lower in the IV metoprolol group (7% vs. 20%, p = 0.012). At a median follow-up of 2 years, occurrence of the pre-specified composite of death, heart failure admission, reinfarction, and malignant arrhythmias was 10.8% in the IV metoprolol group versus 18.3% in the control group, adjusted hazard ratio (HR): 0.55; 95% CI: 0.26 to 1.04; p = 0.065. Heart failure admission was significantly lower in the IV metoprolol group (HR: 0.32; 95% CI: 0.015 to 0.95; p = 0.046).
In patients with anterior Killip class ≤II STEMI undergoing pPCI, early IV metoprolol before reperfusion resulted in higher long-term LVEF, reduced incidence of severe LV systolic dysfunction and ICD indications, and fewer heart failure admissions. (Effect of METOprolol in CARDioproteCtioN During an Acute Myocardial InfarCtion. The METOCARD-CNIC Trial; NCT01311700).
Abstract
We introduce a galaxy cluster mass observable, μ⋆, based on the stellar masses of cluster members, and we present results for the Dark Energy Survey (DES) Year 1 (Y1) observations. Stellar ...masses are computed using a Bayesian model averaging method, and are validated for DES data using simulations and COSMOS data. We show that μ⋆ works as a promising mass proxy by comparing our predictions to X-ray measurements. We measure the X-ray temperature–μ⋆ relation for a total of 129 clusters matched between the wide-field DES Y1 redMaPPer catalogue and Chandra and XMM archival observations, spanning the redshift range 0.1 < $z$ < 0.7. For a scaling relation that is linear in logarithmic space, we find a slope of α = 0.488 ± 0.043 and a scatter in the X-ray temperature at fixed μ⋆ of $\sigma _{{\rm ln} T_\mathrm{ X}|\mu _\star }= 0.266^{+0.019}_{-0.020}$ for the joint sample. By using the halo mass scaling relations of the X-ray temperature from the Weighing the Giants program, we further derive the μ⋆-conditioned scatter in mass, finding $\sigma _{{\rm ln} M|\mu _\star }= 0.26^{+ 0.15}_{- 0.10}$. These results are competitive with well-established cluster mass proxies used for cosmological analyses, showing that μ⋆ can be used as a reliable and physically motivated mass proxy to derive cosmological constraints.
Neck recurrence in papillary thyroid carcinoma DEDIVITIS, ROGÉRIO APARECIDO; MATOS, LEANDRO LUONGO DE; GUIMARÃES, ANDRÉ VICENTE ...
Revista do Colégio Brasileiro de Cirurgiões,
2020, Letnik:
47
Journal Article
Recenzirano
Odprti dostop
ABSTRACT Introduction: papillary thyroid carcinoma is a tumor with good prognosis. However, some patients treated present neck recurrence. Objective: to evaluate the risk factors for neck recurrence. ...Methods: a retrospective study enrolled 89 patients (68 women and 21 men) diagnosed with papillary carcinoma who underwent total thyroidectomy. In 21 patients, neck dissection was performed and 62 patients underwent radioiodinetherapy. Twelve patients relapsed with metastasis in this period with an average of 3.6 years. Results: out of 89 patients, 76.4% were female. Relapse occurred in nine (13.23%) women and three (14.28%) men. The average age of the patients was 44 years in the control group and in patients with relapsed. Eighteen patients (23.37%) in the control group and eight (64.28%) who relapsed had positive lymph nodes at initial diagnosis. The tumor size was significantly larger in the group of patients with cervical recurrence (3.3cm vs. 1.6cm - p=0.008, Student t test), whereas the presence of metastatic lymph nodes at the moment of the first operation was also significant (p=0.004 -Fisher exact test). The tumor size was an independent risk factor for recurrence at the multivariate anaylsis (OR=2.4, IC95%:1.3-4.6 - p=0,007, logistic regression). Conclusion: there is an increase in the risk of lymph node recurrence during the follow up of 2.4 folds for each increase of 1cm in the longer nodule diameter.
RESUMO Introdução: o carcinoma papilífero da tireoide é um tumor com bom prognóstico. Entretanto, alguns pacientes tratados evoluem com recidiva cervical. Objetivo: avaliar os fatores de risco para recidiva cervical. Métodos: um estudo retrospectivo arrolou 89 pacientes (68 mulheres e 21 homens) diagnosticados com carcinoma papilífero, submetidos à tireoidectomia total. Em 21 pacientes, realizou esvaziamento cervical e, em 62, radioiodoterapia. Doze pacientes apresentaram recorrência linfonodal no período, com media de 3,6 anos. Resultados: dos 89 pacientes, 76,4% eram mulheres. A falha ocorreu em nove mulheres (13,23%) e três homens (14,28%). A média etária tanto dos pacientes recidivados como do grupo-controle foi de 44 anos. Dezoito pacientes (23,37%) no grupo-controle e oito (64,28%) dentre os que recidivaram tinham linfonodos positivos ao diagnóstico inicial. O tamanho tumoral foi significativamente maior no grupo de pacientes que apresentaram recidiva cervical (3,3 cm vs. 1,6cm - p=0,008, teste t de Student), o mesmo foi observado para a presença de linfonodos metastáticos quando da primeira cirurgia (p=0,004 - teste exato de Fisher). À análise multivariada, o tamanho tumoral foi fator de risco independente de recidiva (OR=2,4, IC95%:1,3-4,6 - p=0,007, regressão logística. Conclusão: para cada aumento de 1cm no maior diâmetro da lesão, há um aumento de 2,4 vezes no risco de recidiva linfonodal ao longo do acompanhamento.
RESUMO Introdução: A pressão positiva contínua nas vias aéreas nasais (CPAP) é uma forma não invasiva de ventilação utilizada em recém-nascidos prematuros em unidades de terapia intensiva. Porém, ela ...pode acometer o nariz destes pacientes, evoluindo até mesmo com isquemia e necrose columelar. Diversas técnicas são descritas para reconstruir a columela, como enxertos de pele, enxertos compostos, retalhos locais e retalhos livres; mas o enxerto composto condrocutâneo auricular tem se destacado. O objetivo deste trabalho é descrever um caso de necrose de columela por uso de CPAP com reconstrução utilizando enxertia condrocutânea auricular posterior. Relato do Caso: Paciente do sexo feminino, parda, com história de prematuridade e uso prolongado de CPAP quando recém-nascida devido à síndrome da membrana hialina, evoluiu com necrose de columela. Foi submetida à enxertia composta condrocutânea auricular posterior para reconstrução da columela. A paciente apresentou resultado cirúrgico satisfatório, evoluindo com 100% de vitalidade do enxerto. Discussão: A necrose columelar associada ao uso do CPAP pode ser estética e funcionalmente debilitante, e representa um desafio reconstrutivo. As opções para obter resultados aceitáveis são limitadas, porém o uso de enxertos de orelha é tecnicamente simples, utiliza tecidos doadores estruturalmente semelhantes, não provoca cicatrizes adicionais no nariz, é realizado em um tempo cirúrgico e geralmente tem um resultado muito bom. A enxertia composta auricular posterior para reconstrução columelar se mostrou segura, com resultado estético e funcional satisfatório e mínima morbidade da área doadora.
► Development/characterization of nanomultilayer coatings made of food-grade materials. ► Nanomultilayer coatings obtained by layer-by-layer deposition of pectin and chitosan. ► Nanomultilayer ...coatings reduced gas flow and extended the shelf-life of mangoes.
A nanomultilayer coating made of food-grade, bio-based materials (consisting of five nanolayers of pectin and chitosan) was produced. This coating was firstly characterized in terms of the water vapor, oxygen and carbon dioxide permeabilities; these parameters exhibited values of 0.019±0.005×10−11, 0.069±0.066×10−14 and 44.8±32×10−14gm/(Pasm2), respectively, and are of the same order of magnitude of those found in other nanomultilayer systems. The nanomultilayer system was applied on whole ‘Tommy Atkins’ mangoes and the layers’ adsorption was confirmed by changes in the contact angle of the coated fruits’ skin. After 45d of storage, uncoated mangoes presented a higher mass loss, higher total soluble solids and lower titratable acidity in comparison with coated mangoes. Uncoated mangoes had also a damaged and wrinkled appearance, showing evidence of microbial spoilage, and the flesh exhibited a slightly brownish color, in comparison with the coated mangoes. These results suggest a positive effect of the coating on gas flow reduction and on the consequent extension of the shelf-life of mangoes.
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