The viable but nonculturable (VBNC) state has been recognized as a strategy for bacteria to cope with stressful environments; in this state, bacteria fail to grow on routine culture medium but are ...actually alive and can resuscitate into a culturable state under favorable conditions. The VBNC state may pose a great threat to food safety and public health. To date, more than 100 VBNC microorganism species have been proven to exist in fields of food safety, environmental application, and agricultural diseases. Most harsh conditions can induce these microorganisms into the VBNC state, including food processing and preservation methods, adverse environmental conditions, and plant‐disease controlling means. The characteristics of VBNC state cells differ from those of normally growing cells and dead cells, based on which of the various detection methods are developed, and they are of great significance for potential risk assessment. To provide molecular level insights into this state, many studies on induction and resuscitation mechanisms have emerged over the past three decades, including research on omics, specific genes, or proteins involved in VBNC state formation and the roles of promoters in resuscitation from the VBNC state. In this review, microorganism species, induction and resuscitation factors, detection methods, and formation and resuscitation mechanisms of the VBNC state are comprehensively and systematically summarized.
Fullerene derivative, such as 6,6-phenyl C61 butyric acid methyl ester (PCBM), is widely used as an electron-transport layer (ETL) in inverted perovskite solar cell (PSC). However, its low electron ...mobility, complexity in achieving quality film formation, and severe nonradiative recombination at perovskite/PCBM interface due to the large electron capture region, lead to lower efficiency for inverted PSCs compared to the normal structures. Herein, we demonstrate an effective and practical strategy to overcome these challenges. Conjugated n-type polymeric materials are mixed together with PCBM to form a homogeneous bulk-mixed (HBM) continuous film with high electron mobility and suitable energy level. HBM film is found to completely cap the perovskite surface to enhance the electron extraction. The critical electron capture radius of the HBM decreases to 12.52 nm from 14.89 nm of PCBM due to the large relative permittivity, resulting in reduced nonradiative recombination at perovskite/HBM interface. The efficiency of inverted PSCs with HBM ETLs exceeds 20.6% with a high fill factor of 0.82. Further, the stability of devices is improved owing to the high hydrophobicity of the HBM ETLs. Under ambient air condition after 45 days, the efficiency of inverted PSCs based on HBM remains 80% of the initial value. This is significantly higher than the control devices which retain only 48% of the initial value under similar aging conditions. We believe these breakthroughs in improving efficiency and stability of inverted PSCs will expedite their transition.
We propose a scheme to show that the system consisting of two macroscopic oscillators separated in space which are coupled through Coulomb interaction displays the classical-to-quantum transition ...behavior under the action of optomechanical coupling interaction. Once the optomechanical coupling interaction disappears, the entanglement between the two separated oscillators disappears accordingly and the system will return to classical world even though there exists sufficiently strong Coulomb coupling between the oscillators. In addition, resorting to the squeezing of the cavity field generated by an optical parametric amplifier inside the cavity, we discuss the effect of squeezed light driving on this classical-to-quantum transition behavior instead of injecting the squeezed field directly. The results of numerical simulation show that the present scheme is feasible and practical and has stronger robustness against the environment temperature compared with previous schemes in current experimentally feasible regimes. The scheme might possibly help us to further clarify and grasp the classical-quantum boundary.
Even though the power conversion efficiency (PCE) of rigid perovskite solar cells is increased to 22.7%, the PCE of flexible perovskite solar cells (F‐PSCs) is still lower. Here, a novel dimethyl ...sulfide (DS) additive is developed to effectively improve the performance of the F‐PSCs. Fourier transform infrared spectroscopy reveals that the DS additive reacts with Pb2+ to form a chelated intermediate, which significantly slows down the crystallization rate, leading to large grain size and good crystallinity for the resultant perovskite film. In fact, the trap density of the perovskite film prepared using the DS additive is reduced by an order of magnitude compared to the one without it, demonstrating that the additive effectively retards transformation kinetics during the thin film formation process. As a result, the PCE of the flexible devices increases to 18.40%, with good mechanical tolerance, the highest reported so far for the F‐PSCs. Meanwhile, the environmental stability of the F‐PSCs significantly enhances by 1.72 times compared to the device without the additive, likely due to the large grain size that suppresses perovskite degradation at grain boundaries. The present strategy will help guide development of high efficiency F‐PSCs for practical applications.
An efficiency of flexible perovskite solar cells (F‐PSCs) is achieved of 18.40% with small area and 13.35% with large area using effective dimethyl sulfide (DS) additive; both are the highest reported for the F‐PSCs. The F‐PSCs show high efficiency and good stability due to the large grain size and good crystallinity of perovskite when DS is used.
Associations between anxiety, depression and adolescent Internet addiction have been well documented in the literature; however, few published studies have examined these relationships considering ...the developmental trajectory courses of adolescent Internet addiction as well as the individual differences over time. Using a sample of 1545 Chinese adolescents and 3 waves of data over six months, we examined the longitudinal associations between anxiety and depression and Internet addiction, considering sex and obesity. We used latent growth curve modeling (LGCM) to examine the overall conditions of Internet addiction, and latent class growth modeling (LCGM) to determine adolescent developmental membership for Internet addiction. Both unconditional and conditional models were performed. Anxiety and depression were analyzed as time-varying variables, and sex and obesity as time-invariants in our conditional models. Overall, there was a linear decline in adolescent Internet addiction over the six months. Anxiety and depression positively predicted adolescent Internet addiction. Two developmental trajectory patterns for Internet addiction were determined (i.e., low/declining, high/declining). Anxiety was associated with adolescent Internet addiction for both groups of adolescents, but depression was associated with Internet addiction only for adolescents who followed a low/declining course of Internet addiction. Boys reported a higher mean score of Internet addiction at the initial status than girls, and boys also had a faster, declining rate of change over the six months than girls. Obesity was not a predictor of Internet addiction. The results spoke to the importance of considering mental health problems and sex in any intervention efforts to reduce adolescent Internet addiction. Limitations of the study were discussed.
•Anxiety and depression positively are associated with Internet addiction over time.•Adolescents follow different developmental courses of Internet addiction.•Sex is a key variable in understanding Internet addiction.•Obesity is not related to Internet addiction over time.
We propose a scheme to generate squeezed states of magnon and phonon modes and verify squeezing transfer between different modes of distinct frequencies in a cavity magnomechanical system which is ...composed of a microwave cavity and a yttrium iron garnet sphere. We present that by activating the magnetostrictive force in the ferrimagnet, realized by driving the magnon mode with red-detuned and blue-detuned microwave fields, the driven magnon mode can be prepared in a squeezed state. Moreover, the squeezing can be transferred to the cavity mode via the cavity-magnon beamsplitter interaction with strong magnomechanical coupling. We show that under the weak coupling regime, large mechanical squeezing of phonon mode can be achieved, which verifies that our scheme can find the existence of quantum effects at macroscopic scales. Furthermore, distinct parameter regimes for obtaining large squeezing of the magnons and phonons are given, which is the principal feature of our scheme. The considered scheme can be extended to hybrid optical systems, and can facilitate the advancement for realization of strong mechanical squeezing in cavity magnomechanical systems.
In this study, the effect of melatonin on the postharvest ripening and quality improvement of tomato fruit was carried out. The tomatoes were immersed in exogenous melatonin for 2 h, and then the ...related physiological indicators and the expression of genes during post-harvest life were evaluated. Compared with control check (CK) the 50 μM melatonin treatment significantly increased lycopene levels by 5.8-fold. Meanwhile, the key genes involved in fruit colour development, including phytoene synthase1 (PSY1) and carotenoid isomerase (CRTISO), showed a 2-fold increase in expression levels. The rate of water loss from tomato fruit also increased 8.3%, and the expression of aquaporin genes, such as SIPIP12Q, SIPIPQ, SIPIP21Q, and SIPIP22, was up-regulated 2- to 3-fold under 50 μM melatonin treatment. In addition, 50 μM melatonin treatment enhanced fruit softening, increased water-soluble pectin by 22.5%, and decreased protopectin by 19.5%. The expression of the cell wall modifying proteins polygalacturonase (PG), pectin esterase1 (PE1), β-galactosidase (TBG4), and expansin1 (Exp1) was up-regulated under 50 μM melatonin treatment. Melatonin increased ethylene production by 27.1%, accelerated the climacteric phase, and influenced the ethylene signalling pathway. Alteration of ethylene production correlated with altered 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS4) expression. The expression of ethylene signal transduction-related genes such as NR, SIETR4, SIEIL1, SIEIL3, and SIERF2, was enhanced by 50 μM melatonin. The effect of melatonin on ethylene biosynthesis, ethylene perception, and ethylene signalling may contribute to fruit ripening and quality improvement in tomato. This research may promote the application of melatonin on postharvest ripening and quality improvement of tomato fruit as well as other horticultural productions in the future.
The recent advent of single-cell RNA sequencing (scRNA-seq) has enabled access to the developmental landscape of a complex organ by monitoring the differentiation trajectory of every specialized cell ...type at the single-cell level. A main challenge in this endeavor is dissociating plant cells from the rigid cell walls and some species are recalcitrant to such cellular isolation. Here, we describe the establishment of a simple and efficient protocol for protoplast preparation in
, which includes two consecutive digestion processes with different enzymatic buffers. Using this protocol, we generated viable cell suspensions suitable for an array of expression analyses, including scRNA-seq. The universal application of this protocol was further tested by successfully isolating high-quality protoplasts from multiple organs (petals, fruits, tuberous roots, and gynophores) from representative species on the key branches of the angiosperm lineage. This work provides a robust method in plant science, overcoming barriers to isolating protoplasts in diverse plant species and opens a new avenue to study cell type specification, tissue function, and organ diversification in plants.
Visible-light-facilitated phosphorus radical reactions have been developed as a powerful and sustainable tool for the synthesis of various organophosphorus compounds. In general, these reactions ...require stoichiometric amounts of oxidants, and reductants, bases, and radical initiators, leading to uneconomical and complicated processes. Progress has been made over the past few years toward using reactions that proceed under eco-benign and mild reaction conditions. Furthermore, these reactions have broad functional group tolerance, with some facile and economical pathways. Herein, we summarize the discoveries and achievements pertaining to C–P bond formation through a visible light photocatalysis procedure with high atom economy, made by our group and other research groups. It was established that greener and more environmentally friendly approaches do not require an additional oxidant or base. Moreover, we have designed and synthesized a new type of P-radical precursor, which can take part in reactions without the requirement for any additional bases, oxidants, and additives. This breakthrough, pertaining to novel visible-light-induced transformations, will be discussed and a plausible mechanism is proposed, based on corresponding experiments and the literature.
The information-rich vessel movement data provided by the Automatic Identification System (AIS) has gained much popularity over the past decade, during which the employment of satellite-based ...receivers has enabled wide coverage and improved data quality. The application of AIS data has developed from simply navigation-oriented research to now include trade flow estimation, emission accounting, and vessel performance monitoring. The AIS now provides high frequency, real-time positioning and sailing patterns for almost the whole world's commercial fleet, and therefore, in combination with supplementary databases and analyses, AIS data has arguably kickstarted the era of digitisation in the shipping industry. In this study, we conduct a comprehensive review of the literature regarding AIS applications by dividing it into three development stages, namely, basic application, extended application, and advanced application. Each stage contains two to three application fields, and in total we identified seven application fields, including (1) AIS data mining, (2) navigation safety, (3) ship behaviour analysis, (4) environmental evaluation, (5) trade analysis, (6) ship and port performance, and (7) Arctic shipping. We found that the original application of AIS data to navigation safety has, with the improvement of data accessibility, evolved into diverse applications in various directions. Moreover, we summarised the major methodologies in the literature into four categories, these being (1) data processing and mining, (2) index measurement, (3) causality analysis, and (4) operational research. Undoubtedly, the applications of AIS data will be further expanded in the foreseeable future. This will not only provide a more comprehensive understanding of voyage performance and allow researchers to examine shipping market dynamics from the micro level, but also the abundance of AIS data may also open up the rather opaque aspect of how shipping companies release information to external authorities, including the International Maritime Organization, port states, scientists and researchers. It is expected that more multi-disciplinary AIS studies will emerge in the coming years. We believe that this study will shed further light on the future development of AIS studies.
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