Deer (Cervidae) populations are increasing across the temperate zone, threatening forest biodiversity and ecosystem services. However, the fundamental question of what factors make a forest ...susceptible to deer damage remains unanswered, hampering efforts to mitigate against damage. Previous research has assumed that deer density consistently increases forest damage. However, the effect of deer density is likely to be contingent on a range of other drivers, such as climate and landscape structure. Mitigation measures are expensive, so a sound understanding of these contingencies is required to inform the targeting of appropriate management to forests most at risk of damage.
Using records of forest damage from Britain's National Forest Inventory, which comprises over 15,000 plots spanning environmental gradients, we applied a multiscale approach to identify the drivers of forest susceptibility to damage by deer.
Damage likelihood was dependent on drivers operating at multiple spatial scales and their interactions. Broadleaved stands with low tree densities and old trees were consistently found to be at high risk across Britain. The statistical influence of surrounding forest cover within 500 m of a site was modified by regional deer density and climate, in addition to landscape‐level perennial cover. The effect of deer density on damage was therefore not straightforward, but context dependent.
Synthesis and applications. We predict the likely outcome of afforestation initiatives on the likelihood that a forest site will be damaged by deer, with important implications for management and landscape planning in Britain. The complex interactive effects uncovered are difficult to interpret. We therefore provide an interactive Deer Damage Tool (R Shiny application; https://spake.shinyapps.io/DEERDAMAGETOOL/) for practitioners to visualize how afforestation is likely to influence the probability of deer damage in different forests and regions across Britain.
We predict the likely outcome of afforestation initiatives on the likelihood that a forest site will be damaged by deer, with important implications for management and landscape planning in Britain. The complex interactive effects uncovered are difficult to interpret. We therefore provide an interactive Deer Damage Tool (R Shiny application; https://spake.shinyapps.io/DEERDAMAGETOOL/) for practitioners to visualize how afforestation is likely to influence the probability of deer damage in different forests and regions across Britain.
•We describe a model to plan and manage greenspace as a functional green network.•Two profiles: a leisure user and a utilitarian user identify access to greenspace.•Conventional methods may ...substantially overestimate greenspace provision/access.•Outputs used by planners to target health inequalities and promote active travel.
Well planned and managed greenspaces enhance urban environments, providing opportunities for people to relax and to engage with nature. However, greenspace provision has typically focussed upon meeting set targets related to proximity of residential areas and been given a low priority within economic development, after transport, housing and business. In applying proximity criteria, most planning authorities have used a uniform (Euclidean) distance buffer as this is a relatively simple procedure within a Geographic Information System. Such approaches to greenspace may limit its potential, particularly as part of a green network resource, which considers the movement of people. We describe a modelling approach to plan and manage greenspace as a functional green network. Our approach incorporates data on the type and quality of each greenspace and examines the use of greenspace and connecting routes through the perspective of two user profiles: a leisure user and a utilitarian user. These profiles are mapped to represent use based upon the existing green network resource and compared with the conventional Euclidean buffer approach. Our results suggest that conventional methods may substantially overestimate provision/accessibility to greenspace (by a factor of three for leisure greenspace). This is particularly the case when examining who has access; a concern in areas of higher social deprivation where Government initiatives attempt to address health inequalities. The green network approach can help plan and manage where improvements to greenspace quality and access can be targeted to promote regular exercise through incorporating opportunities for active travel and improving daily interaction with greenspace.
The environmental heterogeneity–biodiversity relationship is generally hypothesised to be positive, with greater heterogeneity leading to greater biodiversity. However, the generality of positive ...environmental heterogeneity–species richness relationships is often debated, with some studies finding non-significant or even negative relationships. Negative relationships have primarily been found at fine spatial scales. Both negative and positive relationships have a basis in ecological theory. Environmental heterogeneity at coarse scales opens up niche space to allow more species to coexist; whereas high local heterogeneity, for instance in topography, may lead to increased local extinction due to micro-fragmentation, or dominance of species suited to heterogeneous conditions. However, it is difficult to attribute how much of the variance is explained at different scales within the same modelling framework.
Here, we use a new data-aggregation method which enables us to include both fine- and coarse-scale environmental heterogeneity within the same analysis. Using this method, we were able to tease apart the fine- and coarse-grain effects of topographic heterogeneity on European tree species richness. At the coarse scale (0.5 degrees), we found a positive effect of range in elevation on tree species richness. However, when measuring range in elevation using a fine-scale moving window of radius 500 m, we found a negative relationship with tree species richness. This supports existing research that has shown negative relationships between environmental heterogeneity and species richness at finer spatial grains. Because we were able to include a measure of both local and landscape-scale topographic heterogeneity in the same model, for the first time we could fully capture the effects of both scales on coarse-grain species richness while accounting for the effect of the other scale.
Phenylpropanoids are the precursors to a range of important plant metabolites such as the cell wall constituent lignin and the secondary metabolites belonging to the flavonoid/stilbene class of ...compounds. The latter class of plant natural products has been shown to function in a wide range of biological activities. During the last few years an increasing number of health benefits have been associated with these compounds. In particular, they demonstrate potent antioxidant activity and the ability to selectively inhibit certain tyrosine kinases. Biosynthesis of many medicinally important plant secondary metabolites, including stilbenes, is frequently not very well understood and under tight spatial and temporal control, limiting their availability from plant sources. As an alternative, we sought to develop an approach for the biosynthesis of diverse stilbenes by engineered recombinant microbial cells.
A pathway for stilbene biosynthesis was constructed in Escherichia coli with 4-coumaroyl CoA ligase 1 4CL1) from Arabidopsis thaliana and stilbene synthase (STS) cloned from Arachis hypogaea. E. coli cultures expressing these enzymes together converted the phenylpropionic acid precursor 4-coumaric acid, added to the growth medium, to the stilbene resveratrol (>100 mg/L). Caffeic acid, added in the same way, resulted in the production of the expected dihydroxylated stilbene, piceatannol (>10 mg/L). Ferulic acid, however, was not converted to the expected stilbene product, isorhapontigenin. Substitution of 4CL1 with a homologous enzyme, 4CL4, with a preference for ferulic acid over 4-coumaric acid, had no effect on the conversion of ferulic acid. Accumulation of tri- and tetraketide lactones from ferulic acid, regardless of the CoA-ligase expressed in E. coli, suggests that STS cannot properly accommodate and fold the tetraketide intermediate to the corresponding stilbene structure.
Phenylpropionic acids, such as 4-coumaric acid and caffeic acid, can be efficiently converted to stilbene compounds by recombinant E. coli cells expressing plant biosynthetic genes. Optimization of precursor conversion and cyclization of the bulky ferulic acid precursor by host metabolic engineering and protein engineering may afford the synthesis of even more structurally diverse stilbene compounds.
The yeast-based postbiotic EpiCor is a well-studied formulation, consisting of a complex mixture of bioactive molecules. In clinical studies, EpiCor postbiotic has been shown to reduce intestinal ...symptoms in a constipated population and support mucosal defense in healthy subjects. Anti-inflammatory potential and butyrogenic properties have been reported
, suggesting a possible link between EpiCor's gut modulatory activity and immunomodulation. The current study used a standardized
gut model, the Simulator of the Human Intestinal Microbial Ecosystem (SHIME
), to obtain a deeper understanding on host-microbiome interactions and potential microbiome modulation following repeated EpiCor administration. It was observed that EpiCor induced a functional shift in carbohydrate fermentation patterns in the proximal colon environment. Epicor promoted an increased abundance of
in both the proximal and distal colon, affecting overall microbial community structure. Co-occurrence network analysis at the phylum level provided additional evidence of changes in the functional properties of microbial community promoted by EpiCor, increasing positive associations between Actinobacteria with microbes belonging to the Firmicutes phylum. These results, together with a significant increase in butyrate production provide additional support of EpiCor benefits to gut health. Investigation of host-microbiome interactions confirmed the immunomodulatory potential of the applied test product. Specific microbial alterations were observed in the distal colon, with metabotyping indicating that specific metabolic pathways, such as bile acid and tryptophan metabolism, were affected following EpiCor supplementation. These results, especially considering many effects were seen distally, further strengthen the position of EpiCor as a postbiotic with health promoting functionality in the gut, which could be further assessed
.
A microreactor for electrochemical synthesis has been designed and fabricated. It has been shown that different reactions can be carried out successfully using simple protocols.
Abstract
Colonisation credit refers to the temporal lag between positive conservation actions and species' responses and may be one of the reasons we fail to meet short‐term conservation targets. ...This is particularly evident in woodlands which take decades to develop and harbour slow colonising species. Given global objectives to increase woodland cover, it is important to know the timeframe within which colonisation credit will be fulfilled.
The colonisation of woodland plants was examined in recent woodlands, created between 15 and 80 years ago, and located adjacent or isolated from existing ancient woodlands. Colonisation credit was calculated as the proportion of understory woodland plant species in the nearest ancient woodland which had not colonised recent woodlands. Looking at individual species traits also allowed us to tease apart their impact on the species colonisation and establishment ability.
Spatial adjacency between created and ancient woodland reduced colonisation credit by an average of 28%, and more mature created woodlands (50–80 years old) had fulfilled 24% more of their colonisation credit on average than younger created woodlands (15–21 years old). However, mature woodlands created adjacent to ancient woodlands had still only been colonised by an average of 72% of the available species pool.
Plants which had reached adjacent created woodlands were dispersed by a range of mechanisms, where those that had reached more isolated sites were largely dispersed by birds or mammals. Low community weighted mean shade tolerance, high community weighted nutrient affiliation, and the dominance of
Hedera helix
suggest that competition from dominant natives may be preventing certain species establishing in new woodlands.
This research demonstrates the need to account for appropriate time‐lags when setting biodiversity targets, with most sites still displaying colonisation credit decades after they were created. The results also indicate that spatially targeting woodland creation adjacent to species‐rich mature woodlands should be prioritised. Still, poor local habitat conditions may lead to the dominance of specific competitors which prevent a range of other species from establishing. Local management interventions such as translocations and tree thinning may ameliorate this but further research is needed.
Electrochemical microreactors, which have electrodes integrated into the flow path, can afford rapid and efficient electrochemical reactions without redox reagents due to the intrinsic properties of ...short diffusion distances. Taking advantage of electrochemical microreactors, Kolbe electrolysis of di‐ and trifluoroacetic acid in the presence of various electron‐deficient alkenes was performed under constant current at continuous flow at room temperature. As a result, di‐ and trifluoromethylated compounds were effectively produced in either equal or higher yields than identical reactions under batch conditions previously reported by Uneyamas group. The strategy of using electrochemical microreactor technology is useful for an effective fluoromethylation of alkenes based on Kolbe electrolysis in significantly shortened reaction times.
In the flow! In an electrochemical microreactor, Kolbe electrolysis of acetic acid derivatives allows facile difluoro‐ and trifluoromethylation of electrondeficient alkenes. Compared with bulk reactions, higher yields are achieved at room temperature and in short reaction times.
Least-cost models are widely used to study the functional connectivity of habitat within a varied landscape matrix. A critical step in the process is identifying resistance values for each land cover ...based upon the facilitating or impeding impact on species movement. Ideally resistance values would be parameterised with empirical data, but due to a shortage of such information, expert-opinion is often used. However, the use of expert-opinion is seen as subjective, human-centric and unreliable. This study derived resistance values from grey squirrel habitat suitability models (HSM) in order to compare the utility and validity of this approach with more traditional, expert-led methods. Models were built and tested with MaxEnt, using squirrel presence records and a categorical land cover map for Cumbria, UK. Predictions on the likelihood of squirrel occurrence within each land cover type were inverted, providing resistance values which were used to parameterise a least-cost model. The resulting habitat networks were measured and compared to those derived from a least-cost model built with previously collated information from experts. The expert-derived and HSM-inferred least-cost networks differ in precision. The HSM-informed networks were smaller and more fragmented because of the higher resistance values attributed to most habitats. These results are discussed in relation to the applicability of both approaches for conservation and management objectives, providing guidance to researchers and practitioners attempting to apply and interpret a least-cost approach to mapping ecological networks.
Flavonoids are important plant-specific secondary metabolites synthesized from 4-coumaroyl coenzyme A (CoA), derived from the general phenylpropanoid pathway, and three malonyl-CoAs. The synthesis ...involves a plant type III polyketide synthase, chalcone synthase. We report the cloning and coexpression in Escherichia coli of phenylalanine ammonia lyase, cinnamate-4-hydroxylase, 4-coumarate:CoA ligase, and chalcone synthase from the model plant Arabidopsis thaliana. Simultaneous expression of all four genes resulted in a blockage after the first enzymatic step caused by the presence of nonfunctional cinnamate-4-hydroxylase. To overcome this problem we fed exogenous 4-coumaric acid to induced cultures. We observed high-level production of the flavanone naringenin as a result. We were also able to produce phloretin by feeding cultures with 3-(4-hydroxyphenyl)propionic acid. Feeding with ferulic or caffeic acid did not yield the corresponding flavanones. We have also cloned and partially characterized a new tyrosine ammonia lyase from Rhodobacter sphaeroides. Tyrosine ammonia lyase was substituted for phenylalanine ammonia lyase and cinnamate-4-hydroxylase in our E. coli clones and three different growth media were tested. After 48 h induction, high-level production (20.8 mg L⁻¹) of naringenin in metabolically engineered E. coli was observed for the first time.