ABSTRACT
MicroRNAs (miRNAs) play a crucial role in the growth, development, morphogenesis, signal transduction, and stress response in plants. The ICE (Inducer of CBF expression)‐CBF (C‐repeat ...binding factor)‐COR (Cold‐regulated gene) regulatory cascade is an important signalling pathway in plant response to low temperature stress, and it remains unknown whether this pathway is regulated by miRNAs.
In this study, high‐throughput sequencing was employed for predicting and identifying the miRNAs that were likely to target the ICE‐CBF‐COR pathway in Eucalyptus camaldulensis. A novel ICE1‐targeting miRNA, eca‐novel‐miR‐259‐5p (nov‐miR259), was further analysed.
A total of 392 conserved miRNAs and 97 novel miRNAs were predicted, including 80 differentially expressed miRNAs. Of these, 30 miRNAs were predicted to be associated with the ICE‐CBF‐COR pathway. The full‐length of mature nov‐miR259 was 22 bp and its precursor gene was 60 bp in length, with a typical hairpin structure. The RNA ligase‐mediated 5′ amplification of cDNA ends (5′‐RLM‐RACE) and Agrobacterium‐mediated tobacco transient expression assays demonstrated that nov‐miR259 could cleave EcaICE1 in vivo. Moreover, qRT‐PCR and Pearson's correlation analysis further revealed that the expression levels of nov‐miR259 were almost significantly negatively correlated with those of its target gene, EcaICE1, and the other genes in the ICE‐CBF‐COR pathway.
We first identified the nov‐miR259 as a novel ICE1‐targeting miRNA, and the nov‐miR259‐ICE1 module may be involved in regulating the cold stress response in E. camaldulensis.
We identified a novel miRNA nov‐miR259 in Eucalyptus sp. that could cleave ICE1 and respond to cold stress via the CBF signalling pathway.
High-energy cosmic-ray electrons and positrons (CREs), which lose energy quickly during their propagation, provide a probe of Galactic high-energy processes and may enable the observation of ...phenomena such as dark-matter particle annihilation or decay. The CRE spectrum has been measured directly up to approximately 2 teraelectronvolts in previous balloon- or space-borne experiments, and indirectly up to approximately 5 teraelectronvolts using ground-based Cherenkov γ-ray telescope arrays. Evidence for a spectral break in the teraelectronvolt energy range has been provided by indirect measurements, although the results were qualified by sizeable systematic uncertainties. Here we report a direct measurement of CREs in the energy range 25 gigaelectronvolts to 4.6 teraelectronvolts by the Dark Matter Particle Explorer (DAMPE) with unprecedentedly high energy resolution and low background. The largest part of the spectrum can be well fitted by a 'smoothly broken power-law' model rather than a single power-law model. The direct detection of a spectral break at about 0.9 teraelectronvolts confirms the evidence found by previous indirect measurements, clarifies the behaviour of the CRE spectrum at energies above 1 teraelectronvolt and sheds light on the physical origin of the sub-teraelectronvolt CREs.
There are few data on the molecular epidemiology of cryptococcosis in China. Here we investigated the species distribution, molecular types and antifungal susceptibilities of 312 Cryptococcus ...neoformans species complex isolates from ten hospitals over 5 years. Isolates were identified by internal transcribed spacer (ITS) sequencing and by two matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems. Multilocus sequence typing (MLST) was used to verify species/variety and to designate molecular types. Susceptibility to six antifungal drugs was determined by the Sensititre YeastOne™ method. Cryptococcus neoformans was the predominant species (305/312 isolates (97.8%), all were ITS type 1, serotype A), of which 89.2% (272/305) were C. neoformans var. grubii MLST sequence type (ST) 5 and 6.2% (19/305) were ST31. Other C. neoformans var. grubii STs were rare but included six novel STs. Only two strains were C. neoformans var. neoformans (both serotype AD). Cryptococcus gattii was uncommon (n = 7, four ITS types) and comprised five MLST STs including one novel ST. For C. neoformans var. grubii, the proportion of isolates with non-wild-type MICs to fluconazole significantly rose in the fourth study year (from 0% (0/56 isolates) in the first year to 23.9% (17/71) in the fourth year), including five isolates with fluconazole MICs of ≥32 mg/L. The study has provided useful data on the species epidemiology and their genetic diversity and antifungal susceptibility. The proportional increase in isolates with non-wild-type MICs to fluconazole is noted.
First experimental proof of a clear and strong dependence of the standard phase scintillation index (σφ) derived using Global Positioning System measurements on the ionospheric plasma flow around the ...noon sector of polar ionosphere is presented. σφ shows a strong linear dependence on the plasma drift speed measured by the Super Dual Auroral Radar Network radars, whereas the amplitude scintillation index (S4) does not. This observed dependence can be explained as a consequence of Fresnel frequency dependence of the relative drift and the used constant cutoff frequency (0.1 Hz) to detrend the data for obtaining standard σφ. The lack of dependence of S4 on the drift speed possibly eliminates the plasma instability mechanism(s) involved as a cause of the dependence. These observations further confirm that the standard phase scintillation index is much more sensitive to plasma flow; therefore, utmost care must be taken when identifying phase scintillation (diffractive phase variations) from refractive (deterministic) phase variations, especially in the polar region where the ionospheric plasma drift is much larger than in equatorial and midlatitude regions.
Key Points
A clear strong linear dependence of phase scintillation index on the plasma drift speed around noon sector of the polar ionosphere is presented
Observed dependence can be very possibly explained by the dependence of the shifted Fresnel frequency from the relative drift
Amplifies the importance of using dynamic cutoff frequency in detrending the phase of GPS signal in the polar region
Abstract
We present the second release of value-added catalogues of the LAMOST Spectroscopic Survey of the Galactic Anticentre (LSS-GAC DR2). The catalogues present values of radial velocity Vr, ...atmospheric parameters – effective temperature Teff, surface gravity log g, metallicity Fe/H, α-element to iron (metal) abundance ratio α/Fe (α/M), elemental abundances C/H and N/H and absolute magnitudes MV and $M_{K_{\rm s}}$ deduced from 1.8 million spectra of 1.4 million unique stars targeted by the LSS-GAC since 2011 September until 2014 June. The catalogues also give values of interstellar reddening, distance and orbital parameters determined with a variety of techniques, as well as proper motions and multiband photometry from the far-UV to the mid-IR collected from the literature and various surveys. Accuracies of radial velocities reach 5 km s−1 for the late-type stars, and those of distance estimates range between 10 and 30 per cent, depending on the spectral signal-to-noise ratios. Precisions of Fe/H, C/H and N/H estimates reach 0.1 dex, and those of α/Fe and α/M reach 0.05 dex. The large number of stars, the contiguous sky coverage, the simple yet non-trivial target selection function and the robust estimates of stellar radial velocities and atmospheric parameters, distances and elemental abundances make the catalogues a valuable data set to study the structure and evolution of the Galaxy, especially the solar-neighbourhood and the outer disc.
With the aim of gathering temporal trends on bacterial epidemiology and resistance from multiple laboratories in China, the CHINET surveillance system was organized in 2005. Antimicrobial ...susceptibility testing was carried out according to a unified protocol using the Kirby-Bauer method or automated systems. Results were analyzed according to Clinical and Laboratory Standards Institute (CLSI) 2014 definitions. Between 2005 and 2014, the number of bacterial isolates ranged between 22 774 and 84 572 annually. Rates of extended-spectrum β-lactamase production among Escherichia coli isolates were stable, between 51.7 and 55.8%. Resistance of E. coli and Klebsiella pneumoniae to amikacin, ciprofloxacin, piperacillin/tazobactam and cefoperazone/sulbactam decreased with time. Carbapenem resistance among K. pneumoniae isolates increased from 2.4 to 13.4%. Resistance of Pseudomonas aeruginosa strains against all of antimicrobial agents tested including imipenem and meropenem decreased with time. On the contrary, resistance of Acinetobacter baumannii strains to carbapenems increased from 31 to 66.7%. A marked decrease of methicillin resistance from 69% in 2005 to 44.6% in 2014 was observed for Staphylococcus aureus. Carbapenem resistance rates in K. pneumoniae and A. baumannii in China are high. Our results indicate the importance of bacterial surveillance studies.
We consider the fidelity of the vector meson dominance (VMD) assumption as an instrument for relating the electromagnetic vector-meson production reaction
e
+
p
→
e
′
+
V
+
p
to the purely hadronic ...process
V
+
p
→
V
+
p
. Analyses of the photon vacuum polarisation and the photon-quark vertex reveal that such a VMD
Ansatz
might be reasonable for light vector-mesons. However, when the vector-mesons are described by momentum-dependent bound-state amplitudes, VMD fails for heavy vector-mesons: it cannot be used reliably to estimate either a photon-to-vector-meson transition strength or the momentum dependence of those integrands that would arise in calculations of the different reaction amplitudes. Consequently, for processes involving heavy mesons, the veracity of both cross-section estimates and conclusions based on the VMD assumption should be reviewed, e.g., those relating to hidden-charm pentaquark production and the origin of the proton mass.
Summary
Globozoospermia and acephalic spermatozoa are two rare sperm head anomalies associated with male infertility. Combination of the two phenotypes in the same patient is extremely rare, so the ...underlying pathogenesis of this disorder remains unclear. Here, we report a 35‐year‐old infertile male, who presented with 30% of sperm‐lacked heads and 69% of sperm round‐headed or small‐headed with neck thickening in his ejaculate. Subsequent whole‐exome sequencing (WES) analysis identified compound heterozygous variants within the DNAH6 gene. DNAH6 is a testis‐specific‐expressed protein that was localised to the neck region in the spermatozoa of normal control; however, immunofluorescent staining failed to detect DNAH6 protein in the patient's spermatozoa. Quantitative real‐time PCR analysis also showed the complete absence of DNAH6 mRNA in the patient's spermatozoa. Moreover, two cycles of in vitro fertilisation (IVF)‐assisted reproduction were carried out, but pregnancy was not achieved after embryo transfer. Therefore, rare sequence variants in DNAH6 might be susceptibility risks for human sperm head anomaly.
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