Wuhan was the first large city where the initial breakout of COVID-19 took numerous lives. A group of social workers and mental health specialists coordinated the “Be Together Program” (BTP), a ...psychosocial grief intervention program to help a group of Wuhan COVID-19 bereaved people. Under the Dual-process model framework, BTP used the internet and social media as the main tools, combined with group and individual intervention. Additionally, it employed a “Supermarket Mode” with abundant intervention themes and approaches for BTP participants to choose according to their special needs. Additionally, Chinese cultural elements are integrated into the program. At the end of the program, the grief scores of participants in the qualified sample reduced significantly, and the prevalence of the potential Prolonged Grief Disorder diagnosis reduced from 75% to 12%. The study also found that the BTP was especially effective for those who had high levels of grief reaction.
Based on a new designed acyl hydrazone gelator (G2), we developed a supramolecular organogel in glycol with two different hydrophobic fluorescent dyes, namely rhodamine B (RhB) and acridine red, as ...acceptors. Both the G2@gel-RhB and G2@gel-acridine red systems showed high levels of energy-transfer efficiency and high fluorescence quantum yields.
Based on a acyl hydrazone supramolecular organogel with rhodamine B and acridine red as acceptors. Both the G2@gel-RhB and G2@gel-acridine red systems showed high levels of energy-transfer efficiency and high fluorescence quantum yields.
No study has evaluated the effect of topical powdered vancomycin in patients undergoing primary total knee arthroplasty (TKA). The goal of this study is to determine if this method reduces ...postoperative infection rates following primary TKA.
This retrospective study reviewed 855 consecutive patients undergoing TKA. The first 418 patients, who did not receive topical vancomycin, were grouped into the control group and the subsequent 437 patients, who received powdered vancomycin applied to the target joint prior to wound closure, were grouped into the treatment group.
The control group was found to have 18 infectious complications (4.3%) compared with 6 (1.4%) in the treatment group, which differed significantly (p<0.05). When comparing the rates of infectious complications independently, there was no significant difference in the rate of superficial infection (3.1% vs. 1.4%; p>0.05), while the difference in prevalence of periprosthetic joint infection (PJI) was statistically significant (1.2% vs. 0; p<0.05). No serious adverse events (AEs) occurred.
Topical application of powdered vancomycin may present a reasonable means of decreasing the risk of infectious complications following TKA. There were no serious AEs associated with topical vancomycin. Further research is needed to focus on its long-term efficacy and safety.
III, retrospective, cohort study.
To adapt to the low-velocity water flow closely related to human life, the natural energy can be efficiently harvested and used to power monitoring devices. Herein, a triboelectric soft fishtail ...(TE-SFT) driven by flow-induced vibration (FIV) effect is proposed based on the soft material synthesis technology. Specifically, inspired by the fishtail fin, a bluff body with the cross-section of fishtail-shaped is designed, and has a preferable vortex effect by fluid simulation. In power generation part, the triboelectric nanogenerator (TENG) is designed to act as an inertial pendulum structure by geometric method. Under the FIV effect, the TE-SFT driven by fishtail-shaped bluff body swings like a fish in the water and then brings the inertial pendulum to acquire the oscillation for harvesting energy from low-velocity water flow. The TE-SFT attains an open-circuit voltage (
V
OC
) of 200 V to 313 V at the flow velocities of 0.24 to 0.89 m/s. Additionally, after 30 days of water immersion, the
V
OC
of TE-SFT retains 96.81%. In demonstration, the TE-SFT is applied to power the temperature and humidity sensor through harvesting water flow energy. This work also provides a way for self-powered system based on the TENG and soft bionic fish in water environment.
Owing to the high efficiency of converting nitrate to nitrogen gas with ferrous iron as the electron donor, the process of nitrate-dependent ferrous oxidation (NDFeO) has been considered suitable to ...treat wastewater that contains nitrate but lacks organic matter. Meanwhile, arsenic immobilization often has been found during the NDFeO reaction. Thus, it was strongly expected that nitrate and arsenic could be removed simultaneously in co-contaminated wastewater through the NDFeO process. However, in the current work, arsenic was not removed during the NDFeO process when the pH was high (above 8), though the nitrate reduction rate was over 90%. Meanwhile, the biosolid particles from the NDFeO process demonstrated strong adsorption ability for arsenic when the pH was below 6. Yet, the adsorption became weak when the pH was above 7. Fourier transform infrared (FTIR) spectroscopy analysis revealed that the main activated component for arsenic adsorption was iron oxide in these particles, which was easily crippled under high pH conditions. These results implied that co-removal of nitrate and arsenic in wastewater treatment using NDFeO was difficult to carry out under high pH conditions. Thus, a two-step approach in which nitrate was removed first by NDFeO followed by arsenic adsorption with NDFeO biosolids was more feasible.
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•Nitrate-dependent ferrous oxidation (NDFeO) at high pH could not effectively clear As.•The co-removal of nitrate and As in wastewater treatment with NDFeO was difficult.•Biosolid particles from the NDFeO process proved to be excellent As adsorbents.•For removal of nitrate and As, a two-step approach based on NDFeO is presented.
Glioma is the most common primary tumor of the central nervous system. The conventional glioma treatment strategies include surgical excision and chemo- and radiation-therapy. Interferon Gamma ...(IFN-γ) is a soluble dimer cytokine involved in immune escape of gliomas. In this study, we sought to identify IFN-γ-related genes to construct a glioma prognostic model to guide its clinical treatment.
RNA sequences and clinicopathological data were downloaded from The Cancer Genome Atlas (TCGA) and the China Glioma Genome Atlas (CGGA). Using univariate Cox analysis and the Least Absolute Shrinkage and Selection Operator (LASSO) regression algorithm, IFN-γ-related prognostic genes were selected to construct a risk scoring model, and analyze its correlation with the clinical features. A high-precision nomogram was drawn to predict prognosis, and its performance was evaluated using calibration curve. Finally, immune cell infiltration and immune checkpoint molecule expression were analyzed to explore the tumor microenvironment characteristics associated with the risk scoring model.
Four out of 198 IFN-γ-related genes were selected to construct a risk score model with good predictive performance. The expression of four IFN-γ-related genes in glioma tissues was significantly increased compared to normal brain tissue (
< 0.001). Based on ROC analysis, the risk score model accurately predicted the overall survival rate of glioma patients at 1 year (AUC: The Cancer Genome Atlas 0.89, CGGA 0.59), 3 years (AUC: TCGA 0.89, CGGA 0.68), and 5 years (AUC: TCGA 0.88, CGGA 0.70). Kaplan-Meier analysis showed that the overall survival rate of the high-risk group was significantly lower than that of the low-risk group (
< 0.0001). Moreover, high-risk scores were associated with wild-type
, wild-type
, and 1P/19Q non-co-deletion. The nomogram predicted the survival rate of glioma patients based on the risk score and multiple clinicopathological factors such as age, sex, pathological grade, and
Status, among others. Risk score and infiltrating immune cells including CD8 T-cell, resting CD4 memory T-cell, regulatory T-cell (Tregs), M2 macrophages, resting NK cells, activated mast cells, and neutrophils were positively correlated (
< 0.05). In addition, risk scores closely associated with expression of immune checkpoint molecules such as PD-1, PD-L1, CTLA-4, LAG-3, TIM-3, TIGIT, CD48, CD226, and CD96.
Our risk score model reveals that IFN-γ -associated genes are an independent prognostic factor for predicting overall survival in glioma, which is closely associated with immune cell infiltration and immune checkpoint molecule expression. This model will be helpful in predicting the effectiveness of immunotherapy and survival rate in patients with glioma.
Biomarkers have important roles in various physiological functions and disease pathogenesis. As a nucleocytoplasmic DNA virus, Singapore grouper iridovirus (SGIV) causes high economic losses in the ...mariculture industry. Aptamer-Q5-complexed major capsid protein (MCP) in the membrane of SGIV-infected cells can be used as a specific molecular probe to investigate the crucial events of MCP endocytosis into SGIV-infected host cells during viral infection. Chlorpromazine blocks clathrin-mediated endocytosis, and MCP endocytosis into SGIV-infected cells decreased significantly when the cells were pretreated with chlorpromazine. The disruption of cellular cholesterol by methyl-β-cyclodextrin also significantly reduced MCP endocytosis. In contrast, inhibitors of key regulators of caveolae/raft-dependent endocytosis and macropinocytosis, including genistein, Na
+
/H
+
exchanger, p21-activated kinase 1 (PAK1), myosin II, Rac1 GTPase, and protein kinase C (PKC), had no effect on MCP endocytosis. The endocytosis of the biomarker MCP is dependent on low pH and cytoskeletal actin filaments, as shown with various inhibitors (chloroquine, ammonia chloride, cytochalasin D). Therefore, MCP enters SGIV-infected host cells via clathrin-mediated endocytosis, which is dependent on dynamin, cholesterol, low pH, and cytoskeletal actin filaments. This is the first report of a specific aptamer-based probe used to analyze MCP endocytosis into SGIV-infected host cells during viral infection. This method provides a convenient strategy for exploring viral pathogenesis and facilitates the development of diagnostic tools for and therapeutic approaches to viral infection.
A simple and rapid analytical method for the detection of trifloxystrobin, trifloxystrobin acid and tebuconazole in soil, brown rice, paddy plants and rice hulls was established and validated by ...liquid chromatography with tandem mass spectrometry. Acceptable linearity (R2 > 0.99), accuracy (average recoveries of 74.3–108.5%) and precision (intra‐ and inter‐day relative standard deviations of 0.9–8.8%) were obtained using the developed determination approach. In the field trial, the half‐lives of trifloxystrobin and tebuconazole in paddy plants were 5.7–8.3 days in three locations throughout China, and the terminal residue concentrations of trifloxystrobin and tebuconazole were <100 and 500 μg/kg (maximum residue limits set by China), respectively, at harvest, which indicated that, based on the recommended application procedure, trifloxystrobin and tebuconazole are safe for use on rice. The risk assessment results demonstrated that, owing to risk quotient values of both fungicides being <100%, the potential risk of trifloxystrobin and tebuconazole on rice was acceptable for Chinese consumers. These data could provide supporting information for the proper use and safety evaluation of trifloxystrobin and tebuconazole in rice.
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