To ensure the stability of precision of the motorized spindle for five-axis machining center, the thermal error of five-axis machining center was investigated, and the temperature rise-thermal ...deformation model of a thin-wall ring type rotary elastomer was built on the basis of thermo-elastic property. Thus, a model of radial thermal error was con-structed. The relationship between radial error and axial error was also investigated in this paper and the total radial errors were gotten. In addition, the thermal error of the motorized spindle of five-axis machining center was measured by the ball bar in the experiment of thermal error. The motorized spindle total radial error was achieved by this experiment and radial total error analytical model. Finally, a BP neural network algorithm was introduced for thermal error prediction of five-axis machining center, and the precision of prediction of these models was compared and analyzed. The analysis shows that this method is practical and effective.
This systematic review and meta-analysis aimed to determine whether apical patency increases postoperative pain after endodontic therapy. This study explored the degree and incidence of postoperative ...pain during root canal therapy, as well as the number of required analgesic doses. We searched PubMed, Scopus, Embase, Web of Science, Cochrane Library, and gray literature from the date of database inception until May 2023. RevMan 5.4 software was used for data analysis. Twelve studies were considered eligible for meta-analysis. The mean pain scores on days 1 (mean difference MD = -1.69) and 2 (MD = -0.85) differed significantly between the apical patency and non-patency groups. The odds for pain after 24 h were significantly lower (OR 0.59) in the apical patency group than in the non-patency group. Furthermore, the mean number of required analgesic doses was not significantly different between the two groups. In conclusion, apical patency significantly alleviated postoperative pain (low-quality evidence) and reduced the incidence of pain (moderate evidence). However, high-quality randomized controlled trials are required to validate these findings.
Potato mop-top virus (PMTV) is considered an emerging threat to potato production in the United States. PMTV is transmitted by a soil-borne protist, Spongospora subterranean. Rapid, accurate, and ...sensitive detection of PMTV in leaves and tubers is an essential component in PMTV management program. A rapid test that can be adapted to in-field, on-site testing with minimal sample manipulation could help in ensuring the sanitary status of the produce in situations such as certification programs and shipping point inspections. Toward that goal, a rapid and highly sensitive recombinase polymerase amplification (RPA)-based test was developed for PMTV detection in potato tubers. The test combines the convenience of RPA assay with a simple sample extraction procedure, making it amenable to rapid on-site diagnosis of PMTV. Furthermore, the assay was duplexed with a plant internal control to monitor sample extraction and RPA reaction performance. The method described could detect as little as 10 fg of PMTV RNA transcript in various potato tissues, the diagnostic limit of detection (LOQ) similar to that of traditional molecular methods.
Main conclusion
The NAC transcription factor ATAF2 suppresses its own transcription via self-promoter binding. ATAF2 genetically interacts with the circadian regulator CCA1 and phytochrome A to ...modulate seedling photomorphogenesis in
Arabidopsis thaliana
.
ATAF2 (ANAC081) is a NAC (NAM, ATAF and CUC) transcription factor (TF) that participates in the regulation of disease resistance, stress tolerance and hormone metabolism in
Arabidopsis thaliana
. We previously reported that ATAF2 promotes Arabidopsis hypocotyl growth in a light-dependent manner via transcriptionally suppressing the brassinosteroid (BR)-inactivating cytochrome P450 genes
BAS1
(
CYP734A1
, formerly
CYP72B1
) and
SOB7
(
CYP72C1
). Assays using low light intensities suggest that the photoreceptor phytochrome A (PHYA) may play a more critical role in ATAF2-regulated photomorphogenesis than phytochrome B (PHYB) and cryptochrome 1 (CRY1). In addition, ATAF2 is also regulated by the circadian clock. The core circadian TF CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) physically interacts with ATAF2 at the DNA–protein and protein–protein levels, and both differentially suppress BAS1- and SOB7-mediated BR catabolism. In this research, we show that ATAF2 can bind its own promoter as a transcriptional self-repressor. This self-feedback-suppression loop is a typical feature of multiple circadian-regulated genes. Additionally,
ATAF2
and
CCA1
synergistically suppress seedling photomorphogenesis as reflected by the light-dependent hypocotyl growth analysis of their single and double gene knock-out mutants. Similar fluence-rate response assays using
ATAF2
and photoreceptor (
PHYB
,
CRY1
and
PHYA
) knock-out mutants demonstrate that PHYA is required for ATAF2-regulated photomorphogenesis in a wide range of light intensities. Furthermore, disruption of
PHYA
can suppress the BR-insensitive hypocotyl-growth phenotype of
ATAF2
loss-of-function seedlings in the light, but not in darkness. Collectively, our results provide a genetic interaction synopsis of the circadian-clock-photomorphogenesis-BR integration node involving ATAF2, CCA1 and PHYA.
In this study, two phenol compounds, magnolol and honokiol, were extracted from
and identified by LC-MS,
H- and
C-NMR. The magnolol and honokiol were shown to be effective against seven pathogenic ...fungi, including
(Fr.) Keissl,
(Link) Thom,
f.sp. solani,
J. Sheld,
Schltdl.,
Miyabe & G. Yamada, and
J.G. Kühn, with growth inhibition of more than 57%. We also investigated the mechanisms underlying the potential antifungal activity of magnolol and honokiol. The results showed that they inhibited the growth of
in a dose-dependent manner. Moreover, magnolol and honokiol treatment resulted in distorted mycelia and increased the cell membrane permeability of
, as determined by conductivity measurements. These results suggest that magnolol and honokiol are potential antifungal agents for application against plant fungal diseases.
•Polymer–drug conjugates provide a new platform for drug discovery.•We discuss recent advances in five key classes of polymer–drug conjugates.•Promising approaches to future development of ...polymer–drug conjugates are indicated.
Polymer conjugation is an efficient approach to improve therapeutic properties of drugs and biological agents. Since the first synthetic polymer–drug conjugate entered clinical trials in 1994, this technology has undergone notable development for the introduction and study of novel polymers and for the progress in the biological rationale for designing conjugates. Not surprisingly, new polymers, in addition to the best known polyethylene glycol, polyN-(2-hydroxypropyl)methacrylamide, are continuously conjugated with drugs to achieve biodegradable, stimuli-sensitive and targeted systems in an attempt to prolong blood circulation times and enhance drug concentrations at the intended site of action. This overview focuses on bioconjugates of water-soluble polymers with low molecular weight drugs. Additionally, the most recent achievements in the polymer–drug conjugate field and several promising approaches for the future are discussed.
Whitefly-transmitted begomoviruses cause serious damage to many economically important food, feed, and fiber crops. Numerous vegetable crops are severely affected and chilli leaf curl virus (ChiLCV) ...is the most dominant and widely distributed begomovirus in chilli (Capsicum annuum) throughout the Indian subcontinent. Recently, CRISPR-Cas9 technology was used as a means to reduce geminivirus replication in infected plants. However, this approach was shown to have certain limitations such as the evolution of escape mutants. In this study, we used a novel, multiplexed guide RNA (gRNA) based CRISPR-Cas9 approach that targets the viral genome at two or more sites simultaneously. This tactic was effective in eliminating the ChiLCV genome without recurrence of functional escape mutants. Six individual gRNA spacer sequences were designed from the ChiLCV genome and in vitro assays confirmed the cleavage behaviour of these spacer sequences. Multiplexed gRNA expression clones, based on combinations of the above-mentioned spacer sequences, were developed. A total of nine-duplex and two-triplex CRISPR-Cas9 constructs were made. The efficacy of these constructs was tested for inhibition of ChiLCV infection in Nicotiana benthamiana. Results indicated that all the constructs caused a significant reduction in viral DNA accumulation. In particular, three constructs (gRNA5+4, gRNA5+2 and gRNA1+2) were most effective in reducing the viral titer and symptoms. T7E1 assay and sequencing of the targeted viral genome did not detect any escape mutants. The multiplexed genome-editing technique could be an effective way to trigger a high level of resistance against begemoviruses. To our knowledge, this is the first report of demonstrating the effectiveness of a multiplexed gRNA-based plant virus genome editing to minimize and eliminate escape mutant formation.
The number of CKD patients is on the rise worldwide, and diet has become an essential aspect influencing the treatment and prognosis of CKD. However, limited research has explored the association of ...the Dietary Inflammatory Index (DII) with CKD progression and the essential kidney function indicator, eGFR, in CKD patients. This study aimed to analyze the association between DII and CKD progression and eGFR in the US CKD population using data from the National Health and Nutrition Examination Survey (NHANES).
This study utilized data obtained from the National Health and Nutrition Examination Survey (NHANES) spanning from 2007 to 2018, with a total sample size of 2,488 individuals. Study used multiple imputation, based on 5 replications and a chained equation approach method in the R MI procedure, to account for missing data. Weighted multiple logistic regression was used to analyze the relationship between DII and the risk of higher CKD stage and a weighted multiple regression analysis was used to assess the relationship between DII and eGFR. Weighted Generalized Additive Models and smoothed curve fitting were applied to detect potential non-linear relationships in this association.
In all three models, it was found that DII was positively associated with the risk of higher CKD stage (P < 0.0001), and an increase in DII was associated with a decrease in eGFR (P < 0.0001). The trend across quartiles of DII remained statistically significant, revealing a gradual elevation in higher CKD stage risk and reduction in eGFR levels for the second, third, and fourth quartiles compared to the lowest quartile (P for trend < 0.0001). Upon adjusting for age, gender, race, education level, poverty income ratio (PIR), marital status, body mass index (BMI), metabolic equivalent (MET) score, drinking, smoking, history of hypertension, history of diabetes, cotinine, systolic blood pressure, diastolic blood pressure, total triglycerides, and total cholesterol, we found a positive correlation between DII and the risk of higher CKD stage (OR = 1.26, 95% CI: 1.14-1.40). Further investigation revealed that an increase in DII was associated with a decrease in eGFR (β = -1.29, 95% CI: -1.75, -0.83). Smooth curves illustrated a non-linear positive correlation between DII and CKD risk, while a non-linear negative correlation was observed between DII and eGFR.
Our study results indicate that an increase in DII is associated with an increased risk of higher CKD stage and a decrease in eGFR in all three models. In the fully adjusted model, the risk of higher CKD stage increased by 26% and the eGFR decreased by 1.29 ml/min/1.73 m2 for each unit increase in DII. This finding suggests that in patients with CKD in the US, improved diet and lower DII values may help slow the decline in eGFR and delay the progression of CKD.
A superhydrophobic surface is an ideal choice to enhance the corrosion resistance of aluminum (Al) and Al-based alloys which are widely used in various industrial fields. However, there are ...limitations in the complex fabrication process, high cost, and easy damage of micro-nano structures for processing superhydrophobic Al surface. To address the aforementioned limitations, we presented an efficient and facile method via acid wet blasting method to fabricate micro-nano composite structures on the Al substrate and subsequently low surface energy modification to obtain superhydrophobic Al surface (SHP-AWBM-Al), which had a water contact angle of 162° and rolling angle of 3°. The fabricated SHP-AWBM-Al had excellent self-cleaning performance, anti-fouling ability, and good fog collection ability. Tape peeling test, sandpaper friction test, acidic solution corrosion test, alkaline solution corrosion test, and natural environment corrosion test showed that the SHP-AWBM-Al had good mechanical stability and chemical stability compared with the superhydrophobic Al surface (SHP-H-Al) obtained by chemical etching. In addition, the electrochemical corrosion test showed that the SHP-AWBM-Al was more corrosion-resistant than the SHP-H-Al. This finding could enhance the technology for fabricating superhydrophobic Al surfaces.
In recent years, Meloidogyne enterolobii has emerged as a major parasitic nematode infesting many plants in tropical or subtropical areas. However, the regions of potential distribution and the main ...contributing environmental variables for this nematode are unclear. Under the current climate scenario, we predicted the potential geographic distributions of M. enterolobii worldwide and in China using a Maximum Entropy (MaxEnt) model with the occurrence data of this species. Furthermore, the potential distributions of M. enterolobii were projected under three future climate scenarios (BCC-CSM2-MR, CanESM5 and CNRM-CM6-1) for the periods 2050s and 2090s. Changes in the potential distribution were also predicted under different climate conditions. The results showed that highly suitable regions for M. enterolobii were concentrated in Africa, South America, Asia, and North America between latitudes 30°S to 30°N. Bio16 (precipitation of the wettest quarter), bio10 (mean temperature of the warmest quarter), and bio11 (mean temperature of the coldest quarter) were the variables contributing most in predicting potential distributions of M. enterolobii. In addition, the potential suitable areas for M. enterolobii will shift toward higher latitudes under future climate scenarios. This study provides a theoretical basis for controlling and managing this nematode.
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