Good balance between product and service is the key in the innovative design of product service systems (PSS). In this study, the evolution route of the PSS based on Teoriya Resheniya ...Izobretatelskikh Zadatch ideal final result was provided. The function model of the PSS was constructed according to the service blueprint and function system diagrams. On this basis, an innovation design method of the PSS based on function incentive was established. The function incentive strategies included function synergy, function supplement, and function substitution. Finally, the PSS design process of agricultural machinery based on computer-aided innovation platform was analyzed to verify this method.
•We identified a novel layer of 5S rRNA regulation.•miR-150 and miR-383 inhibit esophageal cancer proliferation by silencing of 5S rRNA.•5S rRNA silencing by miR-150 and miR-383 might intensify ...rpL11–c-Myc interaction.
5S rRNA plays an important part in ribosome biology and is over-expression in multiple cancers. In this study, we found that 5S rRNA is a direct target of miR-150 and miR-383 in esophageal squamous cell carcinoma (ESCC). Overexpression of miR-150 and miR-383 inhibited ESCC cell proliferation in vitro and in vivo. Moreover, 5S rRNA silencing by miR-150 and miR-383 might intensify rpL11–c-Myc interaction, which attenuated role of c-Myc as an oncogenic transcriptional factor and dysregulation of multiple c-Myc target genes. Taken together, our results highlight the involvement of miRNAs in ribosomal regulation during tumorigenesis.
The occurrence characteristics of sodium and its potential relationship with macerals in Zhundong coal, as well as in Shenfu coal with average sodium levels, are investigated in this study. A new ...five-step sequential extraction method was first conducted for determination. The occurrence status of sodium and its related macerals in samples was determined by microscope and SEM–EDS (scanning electron microscope–energy dispersive X-ray spectroscopy). Soluble sodium salts (H2O-Na) make up the primary proportion of sodium in Zhundong coal, at approximately 50%, while various sodium species are distributed in Shenfu coal with nonobvious differences. Inertinite contains more sodium than vitrinite does in both coals, and the highest enrichment degree of sodium was discovered in inertinite from Zhundong coal, which presented primarily as NaCl crystals (H2O-Na) in fusinite cells, with a local weight percentage of over 15%. More specifically, H2O-Na and insoluble Na both tend to enrich in fusinite. Additionally, it is found that maceral-rich products can be gathered using triboelectrostatic separation, and a portion of sodium can thus be removed from the coal by removing inertinite. This study may provide new insights and references regarding sodium removal from Zhundong coal.
Establishing a valid in vitro model to represent tumor heterogeneity and biology is critical but challenging. Tumor organoids are self‐assembled three‐dimensional cell clusters which are of great ...significance for recapitulating the histopathological, genetic, and phenotypic characteristics of primary tissues. The organoid has emerged as an attractive in vitro platform for tumor biology research and high‐throughput drug screening in cancer medicine. Organoids offer unique advantages over cell lines and patient‐derived xenograft models, but there are no standardized methods to guide the culture of organoids, leading to confusion in organoid studies that may affect accurate judgments of tumor biology. This review summarizes the shortcomings of current organoid culture methods, presents the latest research findings on organoid standardization, and proposes an outlook for organoid modeling.
As a heterogeneous kind of malignances, Non-Hodgkin lymphoma (NHL) is the most common hematologic cancer worldwide with the significantly increased morbidity in China. Accumulated evidences ...demonstrated that oncoprotein MDM4 plays a crucial role in the TP53 tumor suppressor signaling pathway. An rs4245739 A>C polymorphism locating in the MDM4 3'-untranslated region creates a miR-191 target site and results in allele-specific MDM4 expression. In this study, we examined the association between this polymorphism as well as the TP53 Arg72Pro (rs1042522 G>C) genetic variant and Non-Hodgkin Lymphoma (NHL) risk in a Chinese Han population. Genotypes were determined in 200 NHL cases and 400 controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by logistic regression. We found significantly increased NHL risk among carriers of the TP53 72Pro allele compared with those with the 72Arg allele (P = 0.002 for the Pro/Pro genotype). We also observed a significantly decreased NHL risks among carriers of the MDM4 rs4245739 C allele compared with those with the A allele in Chinese (P = 0.014 for the AC genotype). Stratified analyses revealed the associations between these SNPs and NHL risk are especially noteworthy in young or male individuals. Additionally, the associations are much pronounced in NHL patients with B-cell lymphomas or grade 3 or 4 disease. Our results indicate that the TP53 Arg72Pro and the MDM4 rs4245739 polymorphisms contribute to NHL susceptibility and support the hypothesis that genetic variants in the TP53 pathway genes can act as important modifiers of NHL risk.
In the past decade, biomimetic calcium phosphate (CaP) ceramics have been considered as practicable grafts and biomaterial substitutes in repairing jaw bone defect after tumor resection or traffic ...accident. Nowadays, increasing incidence of biomedical material-associated infection has raised a concern when applying these materials. In this work, a new porous CaP scaffold with antibacterial coating was proposed. This biomimetic scaffold was composited with berberine (BBR), Ag nanoparticles (nAg), and silk fibroin (SF). The microstructures and phase composition of the scaffolds were analyzed. The cytocompatibility and osteogenic potential of the prepared samples were evaluated
. The scaffolds held hierarchical structure: the first-level porous CaP ceramic with micron pores ranged from 250 to 600 µm; the second-level spongy-like structure with abundant capillary pores ranged from 500 nm to 10 µm; and the third-level structure was achieved by filling BBR, nAg, and SF gel coatings into the above porous structures. The experimental results showed that the antimicrobial capability of single BBR coating is inconspicuous. However, the introduction of nAg could significantly promote the antibacterial effect of scaffolds. At the same time, such scaffolds showed improved osteoinductivity. This new biomimetic CaP scaffold with antibacterial and osteoinductive properties may be a promising candidate for bone tissue engineering.
The torque transmission performance of flight simulator with double 3-RRS over-redundant actuation spherical parallel manipulator is analyzed. The flight simulator has 3 rotational degrees of ...freedom,6 actuators,3 redundant actuators are included in 6 actuators. Two totally same spherical parallel manipulators consist of the flight simulator. Because of only one fixed coordinate frame is established,it has one fixed base in general and one moving platform. The whole moving platform is served as the flight simulator capsule. The directional cosines of the branch are determined by anti-symmetric coordinates frame,and on this basis,the Jacobian matrix of the over-redundant actuation spherical parallel manipulator is given. The torque transmission performance index of the double and single 3-RRS over-redundant actuation spherical parallel manipulator are analyzed and its atlas of torque transmission performance are given out,and according to the 3D atlas,the analysis conclusion is given.
Device fingerprint is information about the target computing device for the purpose of identification. The fingerprinting algorithm that how to assimilate the target into an identifier has been well ...studied for more than 20 years. However, the recent obfuscation method against device fingerprinting makes collecting this information more difficult. In order to solve these problems, this paper proposes a new type of fingerprinting method that relies on the resonant frequency response of the gyroscope. Our method first generates an ultrasonic as the trigger signal and obtains the sensor's output through the application program or the web browser as the response. After the sensor output being normalized during the data preprocessing, resonance features are extracted after frequency domain analysis. Finally, the mobile device is identified through these features matching. In the experiment, we test many types of mobile phones to demonstrate that our method is feasible while users are unaware of this process. For instance, we find that some features are stable when the posture of the mobile device or the time change. There are 10 features based on resonance frequency that can help us improve the classification accuracy to 96.5%. The comprehensive experiments demonstrate that our method can effectively distinguish different types of mobile devices. Even if it is the same model of mobile devices with the same type of gyroscopes, this method still has a good performance.
Nano-hydroxyapatite (nano-HA) has been widely studied as a promising biomaterial because of its potential mechanical and biological properties. In this article, different synthesis methods for ...nano-HA were summarized. Key factors for the synthesis of nano-HA, including reactant concentration, effects of temperature, PH, additives, aging time, and sintering, were separately investigated. The biological performances of the nano-HA depend strongly on its structures, morphology, and crystallite sizes. Nano-HA with different morphologies may cause different biological effects, such as protein adsorption, cell viability and proliferation, angiogenesis, and vascularization. Recent research progress with respect to the biological functions of the nano-HA in some specific biological applications are summarized and the future development of nano-sized hydroxyapatite is prospected.
Odontogenesis is fundamentally controlled by the genome. However, epigenetic factors have indispensable effects during odontogenesis. Previous studies have shown that exogenous factors, such as the ...environment, that cause hypomethylation and hypermethylation in DNA may lead to dental differences in monozygotic twin pairs. In addition, abnormalities in epigenetic regulation could induce disruptions in odontogenesis, thereby causing tooth malformation or agenesis.
This review overviews the epigenetic mechanisms involved in odontogenesis with the aim of establishing a fundamental vision of tooth development, which might be useful in further research in odontogenesis and therapy for dental diseases.
We summarized articles about epigenetics in odontogenesis. Here, we present concrete epigenetic regulation mechanisms in odontogenesis that have been reported previously, following the order of microRNA, DNA methylation and histone modification.
Epigenetic factors influence the proliferation, differentiation or apoptosis of cells that play indispensable roles during the process of odontogenesis which have the ability to exquisitely regulate the tooth number, size and shape.
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