Underground mine fires remain a concern for mine operators, posing a health and safety risk to mineworkers. In the last decade, the number of mine fires has decreased significantly. However, dealing ...with an unknown fire in underground mines can be a challenging task, which could lead to a hazardous condition for miners during an evacuation and rescue operation. A timely detection of a mine fire and monitoring its characteristics-namely, size and location-are of great importance in reducing the risk of mine fire injuries. A new, improved fire location algorithm has been developed and integrated into an atmospheric monitoring system (AMS) program by researchers from the U.S. National Institute for Occupational Safety and Health (NIOSH). This paper describes the new fire location model and presents the results of verification fire tests conducted at the Safety Research Coal Mine (SRCM) facility of the Pittsburgh Mining Research Division (PMRD) using the collected AMS data. NIOSH is endeavoring to develop workplace solutions to improve the detection of and reduce the risk of hazardous conditions in mines. The results demonstrate successful application of the improved fire location model and provide a useful tool for solving the problem of unknown fire location and reducing the risk of hazardous conditions.
OBJECTIVETo explore the mechanism of Qingluo Tongbi formula for regulating "immune-bone erosion" in rheumatoid arthritis (RA).METHODSSixty-four RA patients were randomized into two groups to receive ...treatment with oral methotrexate or Qingluo Tongbi Formula for 12 weeks. Flow cytometry was used to analyze the changes in the percentages of CD3-CD19+, CD19+CD27 and CD19+BAFFR+B cell subpopulations in peripheral blood of the patients, and serum levels of B cell activating factor (BAFF), RANKL, RANK and osteoprotegerin (OPG) levels were detected using ELISA. Before and after the treatment, serum levels of β-CTX, TRACP-5b, BGP, BALP, and PINP were measured with ELISA, and bone mineral density was determined with DXEA dual-energy X-ray absorptiometry. In the cell experiment, RAW264.7 cells were induced to differentiated into osteoclasts and treated with Qingluo Tongbi Formula at low-, moderate and high doses (125, 250 and 500 μg/mL, respectively) or with methotrexate (2 μg/mL) for 48 h, and the changes in the expre
The aim of this study was to explore the effect of micro-ribonucleic acid (miR)-146a on acute gouty arthritis rats through Toll-like receptor-4/myeloid differentiation factor 88 (TLR4/MyD88) signal ...transduction pathway.
A total of 30 clean-grade Sprague-Dawley rats were divided into three groups, including agomiR-146a group (n=10), antagomiR-146a group (n=10) and negative control group (NC, n=10). The model was successfully established via a one-time injection of sodium urate into ankle joint cavity. Subsequently, agomiR-146a (10 μL), antagomiR-146a (10 μL) and normal saline (10 μL) were intrathecally injected into rats in the three groups at 1 h before injection and 12 h, 24 h, 48 h and 72 h after injection, respectively. The ankle joint swelling index, joint dysfunction index and joint inflammation index of rats in the three groups were closely monitored. After 72 h of observation, the rats were euthanized, and synovial tissues were collected from the knee joint. The expression and distribution of nuclear factor-κB (NF-κB) in synovial tissues were detected using the immunohistochemical method. Meanwhile, the expression levels of inflammatory factors, including tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) were detected via enzyme-linked immunosorbent assay. Furthermore, the mRNA and protein expression levels of TLR4 and MyD88 were detected via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blotting, respectively.
No statistically significant differences in the joint swelling index, joint dysfunction index, joint inflammation index, TLR4 and MyD88 and related inflammatory factors were found between the NC group and antagomiR-146a group. Compared with the NC group, agomiR-146a group showed markedly reduced ankle joint swelling index (p<0.05). Meanwhile, joint landing behavior and inflammatory swelling were significantly relieved in the agomiR-146a group (p<0.05). The mRNA and protein expression levels of TLR4 and MyD88 were remarkably decreased as well (p<0.05). Furthermore, the expression and distribution of NF-κB in synovial tissues of agomiR-146a group was markedly reduced when compared with the NC group (p<0.05). In addition, agomiR-146a group exhibited significantly lower expression levels of inflammatory factors (TNF-α, IL-1 and IL-6) in synovial tissues (p<0.05).
MiR-146a alleviates joint inflammation of acute arthritis in rats through the TLR4/MyD88/NF-κB signaling pathway, which may become a new therapeutic target.
The paper described a superior method for preparing photodegradation catalysts based on a metallic-organic framework (MOFs) template. Cadmium sulfide (CdS) nanoparticles with high photocatalytic ...activity are a transition metal sulfide semiconductor. Here, the Co
2
Zn
1
–MOF bimetallic template was successfully synthesised by solvothermal conditions and then calcined in air to obtain bimetallic oxides. In addition, a new class of bimetal composite photo-catalytic material (ZnS–Co
3
S
4
/CdS) was successfully synthesized by sulphiding and compounding with CdS nanoparticles. The resulting materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and ultraviolet-visible absorption spectroscopy (UV-Vis) analysis. The photocatalytic activities were also studied, and the photo-degradation mechanism of composite is discussed briefly. The results suggest that the bimetal composite material has good morphology and a suitable size. The bimetallic material composited with nano-CdS particles (ZnS–Co
3
S
4
/CdS) may be a new type of photocatalytic material to treat organic pollutants in water. This work provides a new approach to synthesizing efficient semiconductor photocatalysts based on MOFs template.
To establish a screening system of adult Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) by fluorescence in situ hybridization (FISH) .
Based on the genetic characteristics of ...Ph-like ALL, FISH probes were designed for ABL1, ABL2, JAK2, EPOR, CRLF2, CSF1R, PDGFRB, and P2RY8 gene breakpoints, which were used to screen Ph-like ALL in B-ALL patients without BCR-ABL1, ETV6-RUNX1, MLL, and E2A gene arrangement. Furthermore, it was analyzed in combination with flow immunophenotype, next-generation sequencing for targeted gene mutations, and RNA sequencing (RNA-seq) .
A total of 189 adult B-ALL patients diagnosed in Nanfang Hospital from January 2016 to April 2019 were enrolled in this study. Using FISH and/or PCR, BCR-ABL1, ETV6-RUNX1, MLL, or E2A arrangement was detected in 83 of them, and Ph-like ALL was detected by FISH in the other 106, resulting in the presence of typical gene arrangements of Ph-like ALL in 12 patients (11.3% , 12/106) . Validated by RNA-seq, the sensitivity and specifi
Aims
Singapore grouper iridovirus (SGIV) is a devastating aquaculture virus responsible for heavy economic losses to grouper, Epinephelus sp. aquaculture. The aim of this study was to develop a rapid ...and sensitive detection method for SGIV infections in infected groupers.
Methods and Results
We previously generated DNA aptamers against SGIV‐infected cells. In this study, we established and characterized a novel aptamer (Q3)‐based enzyme‐linked apta‐sorbent assay (ELASA) for the detection of SGIV infection in Epinephelus coioides. The Q3‐based ELASA could detect SGIV infection rapidly in vitro and in vivo, with high specificity and stability. Q3‐based ELASA specifically recognized SGIV‐infected cells, but not other‐virus‐infected cells or uninfected cells. Q3‐based ELASA detected SGIV infection in a dose‐dependent manner at Q3 concentrations as low as 125 nmol l−1. The results in relation to SGIV‐infected cells (5 × 104), incubation time (1 min) and incubation temperature (37°C) demonstrated that Q3‐based ELASA could detect SGIV infection quickly and stably, superior to antibody‐based enzyme‐linked immunosorbent assay. Q3‐based ELASA could detect the presence of SGIV infection in kidney, liver and spleen samples in vivo, at dilutions of 1/50, 1/100 and 1/50 respectively. The complete detection process took 1–2 h.
Conclusions
Q3‐based ELASA could be a useful tool for diagnosing SGIV infection.
Significance and Impact of the Study
This is the first developed aptamer‐based ELASA for detecting SGIV infection, and is widely applicable in grouper aquaculture industry in light of its rapidity, and high specificity and stability.
In this paper, a method for diagnosing defects inside insulated tubular busbars based on LDA optimized multi-scale texture features is proposed to help to guarantee stable operation of the tubular ...busbars and the whole power grid. Firstly, multi-scale PRPD spectrum space was built with the UHF discharge signals of different defects by image pyramid theory. Then first-order, second-order and higher-order texture statistics were extracted from each image in the multi-scale PRPD spectrum space to form multi-scale texture features and LDA algorithm was used to optimize the features. The method was used to make texture features contain more information about partial discharge and help to improve the accuracy of diagnosis. Experiments were conducted on a 40.5kV insulated tubular busbar and CART classification trees were established as a classifier to identify the types of defects. The results of experiments show that this method can identify the defects of insulated tubular busbars accurately.
PAHs were present in higher concentrations in pore water than in surface water.
The distribution of 16 polycyclic aromatic hydrocarbons (PAHs) was determined in water, sediment and pore water of the ...Jiulong River Estuary and Western Xiamen Sea, China. Total PAH concentrations varied from 6.96 to 26.9 μg/l in water, 59–1177 ng/g dry weight in surficial sediments, and 158–949 μg/l in pore water. The PAHs were present in higher levels in pore water than in surface water, due possibly to higher concentrations of dissolved organic carbon or colloids with which the hydrophobic pollutants were strongly associated. Such a concentration gradient implies a potential flux of pollutants from sediment pore water to overlying water. The levels of PAHs in water and pore water were significantly higher than those found in 1998, suggesting recent inputs of these compounds into the area and re-working of sediment phase. The composition pattern of PAHs in the three phases was dominated by high molecular weight PAHs, in particular 5-ring PAHs. The salinity profile of dissolved PAHs suggested that they all behaved non-conservatively due to deviation from the theoretical dilution line. No correlation was found between PAH concentrations in sediment and those in pore water, and the correlation between the partition coefficients of PAHs and sediment organic carbon content was not significant, suggesting the complexity of the partition behaviour of PAHs. As a result of high PAH concentrations in water and pore water, it is likely that they may have caused mortality to certain exposed organisms.
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