LiFePO4 based Lithium-ion batteries are prepared by nonionic waterborne polyurethane with different soft segments which act as binder. FTIR is used to characterize the structure of waterborne ...polyurethanes .The emulsion viscosity, mechanical properties of films are measured. The result shows that, the emulsion viscosity and tensile strength of polyurethane based polyether glycol are smaller than polyurethane based polyester. Charge-discharge, cycle performance and AC impedance spectroscopy measurement indicat that the first charge-discharge efficiency is 92%, the biggest discharge capacity is 115 mAh/g for lithium-ion batteries based on waterborne polyurethane as adhesive which equaled to PVDF, the batteries have a good cycle performance and high cycle efficiency and the impedance of batteries are small than PVDF.
Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of
calpain
-
3
(
CAPN3
) mRNA in a Chinese local chicken ...breed (Sichuan Mountainous Black-boned chicken MB), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the
CAPN3
mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the
CAPN3
mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (
P
< 0.01). Overall, the
CAPN3
mRNA level exhibited a “rise-decline” developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the
CAPN3
mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken
CAPN3
gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.
POU1F1
is an essential factor that regulates the development and reproduction of animal. The objective of the current research was to screen for polymorphism, expression of
POU1F1
and their ...association with carcass quality traits. A total of 126 Erlang mountainous chickens from two strains (SD02 and SD03) were employed for testing. Seventeen single nucleotide polymorphisms (SNPs) were detected, but only two SNPs (g.96217999 T > C and g.96219442 C > T) were associated with carcass quality traits. In SD03 chicken, g.96217999 T > C genotypes were significantly associated with body weight (BW), carcass weight (CW), eviscerated weight (EW), and semi-eviscerated weight (SEW;
P
< 0.05), and was highly significantly associated with breast muscle weight (BMW) and abdominal fat weight (AW;
P
< 0.01). g.96219442 C > T was significantly associated with BW, EW, SEW (
P
< 0.05). However, these two SNPs were not significantly associated with any carcass traits in SD02 chicken. Diplotypes showed that in SD03 chicken, the haplotype C: C was the most favorable haplotype because it was associated with higher BW, CW, SEW, EW, BMW, and AW (
P
< 0.05). On the contrary, haplotype T: T was associated with lower carcass quality traits (
P
< 0.01). In addition, qRT-PCR revealed that at 13 weeks, the
POU1F1
mRNA expression was significantly higher in breast muscle of cock compared to that of hens (
P
< 0.05), whereas there was no significant correlation between
POU1F1
expression and carcass traits. These results suggested that
POU1F1
could be a potential candidate gene for carcass traits in chicken.
Genetic polymorphisms of ribonucleotidereductase M1 (RRM1) was a DNA repair gene, which may affect patients' response to platinum-based chemotherapy or gemcitabine-based chemotherapy. We ...retrospectively assessed whether single nucleotide polymorphisms (SNPs) of RRM1 can be used to predict overall survival (OS), progression free survival and response in nonsmall cell lung cancer (NSCLC) patients treated with platinum-based regimens as first-line chemotherapy.
The genotypes of four tagSNPs (RRM1 -316C > A, RRM1 -269C > A, RRM1 -702G > A and RRM1 -585T > G) were determined by SNaPshot detection technology and sequencing approaches in 184 advanced NSCLC patients by using peripheral blood.
The overall response rate for 178 patients was 40.2% and the disease control rate was 90.2%. In patients who had ever smoked, a significant correlation was observed between the genotype of RRM1 -269C > A and response (P = 0.046). There was a significant difference in response according to the genotype of RRM1 -702G > A (P = 0.043). Using Log-rank test, we found that patients with the allelotype (CC) of RRM1 -269C > A had a shorter OS (P = 0.006) than the allelotype (CA + AA).
The genotype of RRM1 -269C > A was significantly associated with platinum-based chemotherapy sensitivity in smoking patients and can be used to predict OS in advanced NSCLC patients who received platinum-based chemotherapy or gemcitabine-based chemotherapy. And the genotype of RRM1 -702G > A can serve as a biomarker for chemotherapy sensitivity in advanced NSCLC patients.
Novel, tunable solid‐state emitters based on anthracene groups were synthesised and characterised by spectroscopy and elemental analysis. Their solid‐state photoluminescence properties were studied. ...These fluorophores display interesting solid‐state emission properties with an emission at wavelengths ranging from 550 to 650 nm when excited by a 325 nm helium–cadmium laser at room temperature. In particular, among them, 1,6‐di(9‐anthryl)hexa‐1,5‐diene‐3,4‐dione, 2‐4‐(2‐benzoxazolyl)phenyl‐4,5‐bis2‐(9‐anthryl)vinyl‐1H‐imidazole and 2,3‐bis2‐(9‐anthryl)vinylquinoxaline show red, yellow and green emission, respectively, at 650, 584 and 550 nm. The results demonstrated that the luminescent colours can be tuned from red to yellow and green by simply varying molecular structure. Besides, 1,6‐di(9‐anthryl)hexa‐1,5‐diene‐3,4‐dione also exhibited an upconverted red fluorescent emission peak at around 675 nm under femtosecond excitation at 800 nm.
Background Natural killer （NK） cells play critical roles in host immune defense, while the quantities and subset distributions may vary among different races. To address the difference, we compared ...these variables among Chinese Han, the Caucasians and the Blacks. The study may provide critical background information for both basic research and clinical investigation. Methods Blood samples collected from populations of different races were tested within 12 hours after collection and subsets of NK cells were characterized using flow cytometry. Results The absolute NK count in the Chinese Han was significantly higher than that in the Caucasian. The Han and Caucasian groups showed higher percentages of cytotoxic subset compared to that of the Black group. The percentage of cytokine-producing subset of Chinese Han group was lower than that of Caucasian and Black groups. Black group had a higher percentage of function-unknown NK subset than that of the Han and Caucasian groups. Conclusion Our data indicated that NK cell count and the distribution of different subsets varied among different races, which should be taken into consideration in related investigations.
Sequential optimization and reliability assessment (SORA) is one of the most popular decoupled approaches to solve reliability-based design optimization (RBDO) problem because of its efficiency and ...robustness. In SORA, the double loop structure is decoupled through a serial of cycles of deterministic optimization and reliability assessment. In each cycle, the deterministic optimization and reliability assessment are performed sequentially and the boundaries of violated constraints are shifted to the feasible direction according to the reliability information obtained in the previous cycle. In this paper, based on the concept of SORA, approximate most probable target point (MPTP) and approximate probabilistic performance measure (PPM) are adopted in reliability assessment. In each cycle, the approximate MPTP needs to be reserved, which will be used to obtain new approximate MPTP in the next cycle. There is no need to evaluate the performance function in the deterministic optimization since the approximate PPM and its sensitivity are used to formulate the linear Taylor expansion of the constraint function. One example is used to illustrate that the approximate MPTP will approach the accurate MPTP with the iteration. The design variables and the approximate MPTP converge simultaneously. Numerical results of several examples indicate the proposed method is robust and more efficient than SORA and other common RBDO methods.
Cellular retinol-binding protein 2 (CRBP2), a vitamin A binding protein that is expressed specifically in small intestinal villus absorptive cells, plays a pivotal role in the vitamin A absorption, ...transport, and metabolism. Considering the fact that egg weight and egg quality were largely affected by the vitamin A levels in chicken, we investigated the genetic polymorphism of CRBP2 gene and the association with egg production traits among 349 Erlang Mountainous Chickens in this study. In the coding sequence (408bp) of four exons, we only detected one nucleotide substitution (c.349T>C) in the third exon, which caused amino acid change (p.117Y>H). The frequencies of three genotypes were 52.43% (TT), 42.41% (TC), and 5.16% (CC), respectively. Among the six studied traits, the age at first egg (AFE), total number of eggs with 300 age (EN), and average egg-laying interval (AEI) differed significantly among genotypes. The CC genotype would be genetically advantageous to improve egg production traits in Erlang Mountainous Chicken. The evolutionary conservation analysis revealed that the p.117Y>H site was high conservative in mammals, which potentially confirm the important biological function of this mutation. To our knowledge, this is first report to study the genetic polymorphism of CRBP2 gene and association with egg production traits in chicken.
Baculoviruses are well known for their potential as biological agents for controlling agricultural and forest pests. They are also widely used as expression vectors in molecular cloning studies. The ...genome sequences of 48 baculoviruses are currently available in NCBI databases. As the number of sequenced viral genomes increases, it is important for the authors to present sufficiently detailed analyses and annotations to advance understanding of them. In this study, the complete genome of Clanis bilineata nucleopolyhedrovirus (ClbiNPV) has been sequenced and analyzed in order to understand this virus better.
The genome of ClbiNPV contains 135,454 base pairs (bp) with a G+C content of 37%, and 139 putative open reading frames (ORFs) of at least 150 nucleotides. One hundred and twenty-six of these ORFs have homologues with other baculovirus genes while the other 13 are unique to ClbiNPV. The 30 baculovirus core genes are all present in ClbiNPV. Phylogenetic analysis based on the combined pif-2 and lef-8 sequences places ClbiNPV in the Group II Alphabaculoviruses. This result is consistent with the absence of gp64 from the ClbiNPV genome and the presence instead of a fusion protein gene, characteristic of Group II. Blast searches revealed that ClbiNPV encodes a photolyase-like gene sequence, which has a 1-bp deletion when compared with photolyases of other baculoviruses. This deletion disrupts the sequence into two small photolyase ORFs, designated Clbiphr-1 and Clbiphr-2, which correspond to the CPD-DNA photolyase and FAD-binding domains of photolyases, respectively.
ClbiNPV belongs to the Group II Alphabaculoviruses and is most closely related to OrleNPV, LdMNPV, TnSNPV, EcobNPV and ChchNPV. It contains a variant DNA photolyase gene, which only exists in ChchNPV, TnSNPV and SpltGV among the baculoviruses.
: The male‐specific Y‐chromosomal short tandem repeat (STR) is a useful tool in forensic casework. The Y haplotype comprised of 16 loci, which is amplified simultaneously by AmpFlSTR® YfilerTM PCR ...kit and provides strong exculpatory evidence in individual identification. We reported a rare Y‐STR profile with a null allele at the DYS448 locus and an off‐ladder allele at the DYS456 locus, when genotyping material from a vaginal swab in an alleged rape case. Sequence analysis revealed that the DYS448 null allele was a true type of null allele because of a total deletion of 11 upstream repeats and 9 bp of the N42 region, and there were numerous primer binding site mutations as well. The amplicon of the DYS456 locus was a small 92‐bp fragment that was off‐ladder, and sequencing analysis showed that there were only 10 repeats (AGAT)10. This Y chromosome haplotype that was comprised of two variations provided helpful evidence for personal identification.
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