Background
Diarrhea‐predominant irritable bowel syndrome (IBS‐D) is a functional gastrointestinal (GI) disorder, which occurs more frequently in women than men. The aim of our study was to determine ...the role of activation of classical estrogen receptors (ER) and novel membrane receptor, G protein‐coupled estrogen receptor (GPER) in human and mouse tissue and to assess the possible cross talk between these receptors in the GI tract.
Methods
Immunohistochemistry was used to determine the expression of GPER in human and mouse intestines. The effect of G‐1, a GPER selective agonist, and estradiol, a non‐selective ER agonist, on muscle contractility was characterized in isolated preparations of the human and mouse colon. To characterize the effect of G‐1 and estradiol in vivo, colonic bead expulsion test was performed. G‐1 and estradiol activity on the visceral pain signaling was assessed in the mustard oil‐induced abdominal pain model.
Key Results
GPER is expressed in the human colon and in the mouse colon and ileum. G‐1 and estradiol inhibited muscle contractility in vitro in human and mouse colon. G‐1 or estradiol administered intravenously at the dose of 20 mg/kg significantly prolonged the time to bead expulsion in females. Moreover, G‐1 prolonged the time to bead expulsion and inhibited GI hypermotility in both genders. The injection of G‐1 or estradiol resulted in a significant reduction in the number of pain‐induced behaviors in mice.
Conclusions and Inferences
GPER and ER receptors are involved in the regulation of GI motility and visceral pain. Both may thus constitute an important pharmacological target in the IBS‐D therapy.
Estrogen receptors (ER) and G protein‐coupled estrogen receptors are involved in colonic motility and visceral pain. GPER is expressed in the human colon. G‐1, a selective GPER agonist, and estradiol, a non‐selective ER agonist, inhibited muscle contractility in vitro in human colon.
Environmental pollution by heavy metals affects both urban and non-urban areas of Europe and the world. The use of bioindicator plants for the detection of these pollutants is a common practice. An ...important property of potential bioindicators is their easy availability and wide distribution range, which means that they can be practically used over a wide area. Therefore, common and widely distributed weeds: Trifolium pratense L., Rumex acetosa L., Amaranthus retroflexus L., Plantago lanceolata L., ornamental species Alcea rosea L., and Lolium multiflorum L. var. Ponto were selected as a potential bioindicators of heavy metals (Cd, Pb, Cu, Zn). Plants were exposed in the same soil conditions in three sample sites in the Poznań city. It was found that all species had heavy metal accumulation potential, especially A. rosea, P. lanceolata and L. multiflorum for Zn (BCF = 6.62; 5.17; 4.70) and A. rosea, P. lanceolata for Cd (BCF = 8.51; 6.94). Translocation of Cu and Zn was the most effective in T. pratense (TF
= 2.55; TF
= 2.67) and in A. retroflexus (TF
= 1.50; TF
= 2.23). Cd translocation was the most efficient in T. pratense (TF
= 1.97), but PB was the most effective translocated in A. retroflexus (TF
= 3.09).. Based on physiological response to stress, it was detected an increasing level of hydrogen peroxide (H
O
) in roots and leaves of all samples, with the highest in all organs of A. rosea. Enzymatic activity levels of CAT, APOX, and also the marker of polyunsaturated fatty acid peroxidation MDA, were higher after 6 weeks of exposure in comparison to control samples and varied in time of exposure and between species and exposure. After the experiment, in almost all samples we detected a reduction of chlorophyll content and relative water content, but in efficiency of photosynthesis parameters: net photosynthesis rate, intercellular CO
concentration and stomatal conductance, we noted increased values, which proved the relatively good condition of the plants. The examined weeds are good bioindicators of heavy metal contamination, and their combined use makes it possible to comprehensively detection of environmental threats.
Abstract
Pulsatilla vernalis
is a IUCN listed species that occurs in mountain and lowland habitats. The seeds collected from different populations are remarkably diverse in their viability depending ...on locality or year of collection. We aim to analyse seed viability, among others, by investigation of the percentage of alive, dying, and dead cells in embryos and endosperm when comparing the seeds from a wild lowland population and ex situ cultivation of plants of lowland and Alpine origin. The cell death was detected by staining with two fluorescence probes, one penetrating only the changed nuclear membranes, the other penetrating also the unchanged cells. 54.5% of Alpine origin seeds were presumably capable of germination if they were sown after collection, however, four months later only 36.4% had healthy embryos. In the case of lowland wild plants it was 31.8% and 18.2%, and from ex situ, 27.3% and 13.6%, respectively. 27.3% of Alpine origin seeds had embryo in torpedo stage (9.1% in the case of lowland seeds). Mean weight of the former was 2.9 mg (2.0 mg in lowland ones). Our results confirm the significance of seed origin and seed weight on viability, and that
Pulsatilla
seeds have a short ‘germination time window’.
Polyploidization drives the evolution of grasses and can result in epigenetic changes, which may have a role in the creation of new evolutionary lineages and ecological speciation. As such changes ...may be inherited, they can also influence adaptation to the environment. Populations from different regions and climates may also differ epigenetically; however, this phenomenon is poorly understood. The present study analyzes the effect of climatic stress on global DNA methylation based on a garden collection of two related mountain grasses (the narrow endemic diploid Festuca tatrae and the more widely distributed mixed-ploidy F. amethystina) with different geographic ranges and ecological niches. A lower level of DNA methylation was observed for F. tatrae, while a higher mean level was obtained for the diploid and tetraploid of F. amethystina; with the tetraploids having a higher level of global methylated DNA than the diploids. The weather conditions (especially insolation) measured 24 h prior to sampling appeared to have a closer relationship with global DNA methylation level than those observed seven days before sampling. Our findings suggest that the level of methylation during stress conditions (drought, high temperature and high insolation) may be significantly influenced by the ploidy level and bioclimatic provenance of specimens; however an important role may also be played by the intensity of stress conditions in a given year.
One promising area in understanding the responses of plants to ongoing global climate change is the adaptative effect of polyploidy. This work examines whether there is a coupling between the ...distribution of cytotypes and their biogeographical niche, and how different niches will affect their potential range. The study uses a range of techniques including flow cytometry, gradient and niche analysis, as well as distribution modelling. In addition, climatic, edaphic and habitat data was used to analyse environmental patterns and potential ranges of cytotypes in the first wide-range study of Festuca amethystina-a mixed-ploidy mountain grass. The populations were found to be ploidy homogeneous and demonstrate a parapatric pattern of cytotype distribution. Potential contact zones have been identified. The tetraploids have a geographically broader distribution than diploids; they also tend to occur at lower altitudes and grow in more diverse climates, geological units and habitats. Moreover, tetraploids have a more extensive potential range, being six-fold larger than diploids. Montane pine forests were found to be a focal environment suitable for both cytotypes, which has a central place in the environmental space of the whole species. Our findings present polyploidy as a visible driver of geographical, ecological and adaptive variation within the species.
The first low-energy Coulomb-excitation measurement of the radioactive, semi-magic, two proton-hole nucleus 206Hg, was performed at CERN's recently-commissioned HIE-ISOLDE facility. Two γ rays ...depopulating low-lying states in 206Hg were observed. From the data, a reduced transition strength B(E2;21+→01+)=4.4(6) W.u. was determined, the first such value for an N=126 nucleus south of 208Pb, which is found to be slightly lower than that predicted by shell-model calculations. In addition, a collective octupole state was identified at an excitation energy of 2705 keV, for which a reduced B(E3) transition probability of 30−13+10 W.u. was extracted. These results are crucial for understanding both quadrupole and octupole collectivity in the vicinity of the heaviest doubly-magic nucleus 208Pb, and for benchmarking a number of theoretical approaches in this key region. This is of particular importance given the paucity of data on transition strengths in this region, which could be used, in principle, to test calculations relevant to the astrophysical r-process.
•Niche modeling using the SoilGrids database is a valuable tool in ecological studies.•Tetraploids have a wider niche breadth when compared to diploids.•Polyploidization under changing environmental ...conditions leads to colonization.•Festuca amethystina extended the geographical range to post-glacial lowland areas.•The soil factor can be key in plant distribution.
In the framework of polyploidization, a widely discussed issue is the possibility of niche shift. Numerous studies have investigated niche differentiation among cytotypes, with some confirming distinct differences, while others finding them to be similar. Within this research project, we focus on the case of the cytotypes (2x and 4x) of Festuca amethystina L. and the closely related F. tatrae (Czakó) Degen (2x). We hypothesize that a significant change in the edaphic niche of tetraploids could be a factor that enabled them to occupy new lowland areas inaccessible to diploids which are characteristic of mountain habitats.
The edaphic niche differentiation between Festuca amethystina cytotypes was determined by studying the niches’ breadths using PCA-based modeling and separate analyses of particular soil features. The modeling was based on SoilGridsTMdata, and the conclusions were compared with the results of the direct soil studies. Such a research model makes it possible to determine to what extent the data coming from the mentioned database allows for the indication of niche differentiation.
Coarse-grained modeling based on SoilGridsTM data indicated a significant change in the edaphic requirements of the tetraploids, as well as pointed to the niche shift towards a higher content of sand, lower carbon and nitrogen content, and lower pH. The direct soil sampling and laboratory tests confirmed these trends and provided a more detailed understanding of the cytotypes requirements. However, no statistically significant differences were observed between cytotypes concerning soil pH, the majority of base cations, available forms of phosphorus and potassium, and the results of CHN elemental analysis. While the direct soil sampling did not reveal significant differences, the analyses based on the niche modeling, considering a wide background area in Central Europe (using SoilGrids data), captured significant variations. This comprehensive approach allowed us to uncover a highly logical pattern of niche shift, which indicates that tetraploids are predisposed to extend their geographical range to post-glacial lowland areas.
Additionally, Festuca amethystina tetraploids display a wider edaphic niche breadth than their closely related and hypothetical ancestor Festuca tatrae. The decision to include the latter reduced the probability that the obtained result of the significant increase of the tetraploid edaphic niche was a simple result of hybridization.
The article presents a comparative numerical analysis of various ways to strengthen historical brick arches. Five ways of strengthening brick arches with steel tie-rods have been proposed. Two of ...these involve the use of braces wrapped around pillars supporting the arch connected with a tie-rod; the other two ways involve the use of the tie-rods with welded metal sheets of different sizes; the latter involves the use of a tie-rod glued with the use of an epoxy adhesive. The collected data were compared with the reference model of the arch left without any interference. The results make it possible to evaluate the effectiveness of the methods by comparing displacements in the vertical and horizontal direction and stresses. The article indicates the direction of proper planning and design of the arch strengthening in brick structures in historical buildings.
The unstable N=42 nucleus 72Zn has been studied using multiple safe Coulomb excitation in inverse kinematics. The experiment was performed at the REX-ISOLDE facility at CERN making first use of the ...silicon detector array C-REX in combination with the γ-ray spectrometer Miniball. The high angular coverage of C-REX allowed to determine the reduced transition strengths for the decay of the yrast 01+, 21+ and 41+ as well as of the 02+ and 22+ states in 72Zn. The quadrupole moments of the 21+, 41+ and 22+ states were extracted. Using model independent quadrupole invariants, the ground state of 72Zn was found to have an average deformation in the γ degree of freedom close to maximum triaxiality. In comparison to experimental data in zinc isotopes with N<40, the collectivity of the 41+ state in neutron-rich 72Zn is significantly larger, indicating a collective yrast band based on the ground state of 72Zn. In contrast, a low experimental B(E2;02+→21+) strength was determined, indicating a different structure for the 02+ state. Shell-model calculations propose a 02+ state featuring a larger fraction of the (spherical) N=40 closed-shell configuration in its wave function than for the 01+ ground state.
The results were also compared with beyond mean field calculations which corroborate the large deformation in the γ degree of freedom, while pointing to a more deformed 02+ state. These experimental and theoretical findings establish the importance of the γ degree of freedom in the ground state of 72Zn, located between the 68,70Ni nuclei that have spherical ground states, and 76Ge, which has a rigid triaxial shape.
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