Quorum sensing (QS) is a mechanism by which gram-negative bacteria regulate their gene expression by making use of cell density. QS is triggered by a small molecule known as an autoinducer. ...Typically, gram-negative bacteria such as Vibrio produce signaling molecules called acyl homoserine lactones (AHLs). However, their levels are very low, making them difficult to detect. We used thin layer chromatography (TLC) to examine AHLs in different Vibrio species, such as Vibrio alginolyticus, Vibrio parahemolyticus, and Vibrio cholerae, against a standard- Chromobacterium violaceum. Further, AHLs were characterised by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC–MS). C4-HSL (N- butanoyl- L- homoserine lactone), C6-HSL (N- hexanoyl- L- homoserine lactone), 3-oxo-C8-HSL (N-(3-Oxooctanoyl)-DL-homoserine lactone), C8-HSL (N- octanoyl- L- homoserine lactone), C110-HSL (N- decanoyl- L- homoserine lactone), C12-HSL (N- dodecanoyl- L- homoserine lactone) and C14-HSL (N- tetradecanoyl- L- homoserine lactone) were identified from Vibrio. These results may provide a basis for blocking the AHL molecules of Vibrio, thereby reducing their pathogenicity and eliminating the need for antimicrobials.
Aldous Huxley (1894–1963) was the author of nearly fifty books and numerous essays, best known for his dystopian novel Brave New World. Humphry Osmond (1917–2004) was a British-trained psychiatrist ...interested in the biological nature of mental illness and the potential for psychedelic drugs to treat psychoses, especially schizophrenia. In 1953, Huxley sent an appreciative note to Osmond about an article he and a colleague had published on their experiments with mescaline, which inspired an initial meeting and decade-long correspondence. This critical edition provides the complete Huxley-Osmond correspondence, chronicling an exchange between two brilliant thinkers who explored such subjects as psychedelics, the visionary experience, the nature of mind, human potentialities, schizophrenia, death and dying, Indigenous rituals and consciousness, socialism, capitalism, totalitarianism, power and authority, and human evolution. There are references to mutual friends, colleagues, and eminent figures of the day, as well as details about both men's personal lives. The letters bear witness to the development of mind-altering drugs aimed at discovering the mechanisms of mental illness and eventually its treatment. A detailed introduction situates the letters in their historical, social, and literary context, explores how Huxley and Osmond first coined the term "psychedelic," contextualizes their work in mid-century psychiatry, and reflects on their legacy as contributors to the science of mind-altering substances. Psychedelic Prophets is an extraordinary record of a full correspondence between two leading minds and a testament to friendship, intellectualism, empathy, and tolerance. The fact that these sentiments emerge so clearly from the letters, at a historical moment best known for polarizing ideological conflict, threats of nuclear war, and the rise of post-modernism, reveals much about the personalities of the authors and the persistence of these themes today.Aldous Huxley (1894–1963) was the author of nearly fifty books and numerous essays, best known for his dystopian novel Brave New World. Humphry Osmond (1917–2004) was a British-trained psychiatrist interested in the biological nature of mental illness and the potential for psychedelic drugs to treat psychoses, especially schizophrenia. In 1953, Huxley sent an appreciative note to Osmond about an article he and a colleague had published on their experiments with mescaline, which inspired an initial meeting and decade-long correspondence. This critical edition provides the complete Huxley-Osmond correspondence, chronicling an exchange between two brilliant thinkers who explored such subjects as psychedelics, the visionary experience, the nature of mind, human potentialities, schizophrenia, death and dying, Indigenous rituals and consciousness, socialism, capitalism, totalitarianism, power and authority, and human evolution. There are references to mutual friends, colleagues, and eminent figures of the day, as well as details about both men's personal lives. The letters bear witness to the development of mind-altering drugs aimed at discovering the mechanisms of mental illness and eventually its treatment. A detailed introduction situates the letters in their historical, social, and literary context, explores how Huxley and Osmond first coined the term "psychedelic," contextualizes their work in mid-century psychiatry, and reflects on their legacy as contributors to the science of mind-altering substances. Psychedelic Prophets is an extraordinary record of a full correspondence between two leading minds and a testament to friendship, intellectualism, empathy, and tolerance. The fact that these sentiments emerge so clearly from the letters, at a historical moment best known for polarizing ideological conflict, threats of nuclear war, and the rise of post-modernism, reveals much about the personalities of the authors and the persistence of these themes today.
<正>Objective:To isolate the multiple drug resistance(MDR) Mycobacterium tuberculosis(M. tuberculosis) and to screen for the bioactive compounds extracted from marine microalgae. Methods:Sixty ...seven collected samples that were confirmed by 16S rDNA analysis as positive with M.tuberculosis infection were subjected to sensitivity test against commercially used front line and second line drugs by absolute concentration method using LJ slants.Seven bacterial isolates numbered I-78,I-101,I-127,I-173,I-202,I-262,I-327 showed resistant for more than 3 drugs were considered as MDR M.tuberculosis.Thus the percentage of 10.4 were recorded MDR-TB. Fifteen marine micro algal extracts were screened for antimycobacterial activity and partial characterization of the active principles was done.Results:The percentage contribution of marine micro algal species on the extraction of antimycobacterials indicated Isochrysis galbana(I. galbana) contain rich bioactive compounds and accounted for 60%inhibition of the total isolates. The percentage contribution of solvents on the extraction of antimicrobials from I.galbana showed that the methanol,chloroform,n-Butanol showed maximum of extraction.The purified eluted compounds(R,0.43) from TLC plate were chromatographed by gas chromatography.The eluted sterol compounds showed 13 unsaturated sterols with 3 major sterols.Conclusions:The present study indicates the presence of unsaturated fatty acids may have the effect on MDR M. tuberculosis,indicating a potential natural alternative to antibiotics.
Now More Than Ever Huxley, Aldous; Bradshaw, David; Sexton, James
01/2010
eBook
Over the course of his career, British writer Aldous Huxley (1894-1963) shifted away from elitist social satires and an atheistic outlook toward greater concern for the masses and the use of ...religious terms and imagery. This change in Huxley's thinking underlies the previously unpublished play Now More Than Ever. Written in 1932-1933 just after Brave New World, Now More Than Ever is a response to the social, economic, and political upheavals of its time. Huxley's protagonist is an idealistic financier whose grandiose schemes for controlling the means of production drive him to swindling and finally to suicide. His fate allows Huxley to expose the evils he perceives in free-market capitalism while pleading the case for national economic planning and the rationalization of Britain's industrial base. This volume contains the full text of Now More Than Ever, which was believed to be lost until 1976, when a copy was found at the Harry Ransom Humanities Research Center of the University of Texas at Austin. A "thinker's play" that has never been produced on stage, it is the last previously unpublished piece of Huxley's major writings and immensely important to understanding his development as a writer. The editors of this volume have annotated the play for contemporary readers. Their introduction sets the play in the context of Huxley's intellectual life.
Marking the fiftieth anniversary of the death of Aldous Huxley (18941963), Temporaries and Eternals focuses on the music column that Huxley wrote for The Weekly Westminster Gazette in 192223. ...Readers of Huxleys novels, essays and travel writing will be aware of the wealth of musical detail in these works, and this book suggests that such references can only be fully understood in the context of the opinions voiced in Huxleys music criticism. Not only does Huxleys column offer a fascinati.
For a long time past, thinking men have tended to adopt a somewhat patronizing attitude towards the words they use in communicating with their fellows and formulating their own ideas. “What do you ...read, my lord?” Polonius asked. And with all the method that was in his madness Hamlet scornfully replied, “Words, words, words.” That was at the beginning of the seventeenth century; and from that day to this the people who think themselves realists have gone on talking about words in the same contemptuous strain.
There was a reason for this behavior—or at least an excuse. Before the
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