The European green woodpecker, Picus viridis, is a widely distributed species found in the Western Palearctic region. Here, we assembled a highly contiguous genome assembly for this species using a ...combination of short- and long-read sequencing and scaffolded with chromatin conformation capture (Hi-C). The final genome assembly was 1.28 Gb and features a scaffold N50 of 37 Mb and a scaffold L50 of 39.165 Mb. The assembly incorporates 89.4% of the genes identified in birds in OrthoDB. Gene and repetitive content annotation on the assembly detected 15,805 genes and a ∼30.1% occurrence of repetitive elements, respectively. Analysis of synteny demonstrates the fragmented nature of the P. viridis genome when compared to the chicken (Gallus gallus). The assembly and annotations produced in this study will certainly help for further research into the genomics of P. viridis and the comparative evolution of woodpeckers. Five historical and seven contemporary samples have been resequenced and may give insights on the population history of this species.
The foraging behaviour and habitat use of the Eurasian Green Woodpecker
at various sites in Hungary over a 20-year period was documented. Detailed observations were recorded on foraging behaviour at ...hard substrates; in quarries, cliffs and human made structures of brick and stone construction. Using Chi-square tests on the frequency of observations of birds at hard substrates foraging sites, we compared usage during periods of snow cover and those without. Birds were found to be more frequently observed at hard substrates during periods of snow cover because these remained largely free of snow. We supposed that this response was due to invertebrate prey becoming increasingly scarce generally across typical foraging sites, i.e. grasslands and meadows during harsh winter conditions. Accessibility to the alternative sites became important as a source of food because availability of prey was more reliable. Vertical surfaces of hard substrates such as those associated with quarries, cliffs and buildings may be important to sustain Eurasian Green Woodpeckers in periods of snow cover where these provide a valuable foraging resource.
Land use change is a major threat to global biodiversity. Forest species face the dual threats of deforestation and intensification of forest management. In regions where forests are under threat, ...rural landscapes that retain structural components of mature forests potentially provide valuable additional habitat for some forest species. Here, we illustrate the habitat value of traditional wood pastures for a woodpecker assemblage of six species in southern Transylvania, Romania. Wood pastures are created by long-term stable silvo-pastoral management practices, and are composed of open grassland with scattered large, old trees. Because of their demanding habitat requirements, woodpeckers share habitat with many other bird species, and have been considered as possible indicator species for bird species diversity. We first compared woodpecker assemblages between forests and wood pastures. Second, we grouped features of wood pastures into three spatial contexts and addressed how these features related to the occurrence of three woodpecker species that are formally protected. Woodpecker species composition, but not the number of species, differed between forests and wood pastures, with the green woodpecker occurring more commonly in wood pastures, and the lesser spotted woodpecker more commonly in forests. Within wood pastures, the intermediate context (especially surrounding forest cover) best explained the presence of the grey-headed and middle spotted woodpecker. By contrast, variables describing local vegetation structure and characteristics of the surrounding landscape did not affect woodpecker occurrence in wood pastures. In contrast to many other parts of Europe, in which several species of woodpeckers have declined, the traditional rural landscape of Transylvania continues to provide habitat for several woodpecker species, both in forests and wood pastures. Given the apparent habitat value of wood pastures for woodpeckers we recommend wood pastures be explicitly considered in relevant policies of the European Union, namely the Habitats Directive and the EU Common Agricultural Policy.
Nel 2017 e 2018 è stata studiata la dieta del picchio verde (Picus viridis) in due aree del Veneto: la Valle di Schievenin (Quero Vas, Belluno) e il paese di Cendon (Silea, Treviso). La ricerca è ...stata svolta mediante la raccolta delle fatte e la successiva analisi del contenuto. Sono stati raccolti 26 campioni contenenti complessivamente 3.255 individui di formica. Le specie più comuni sono risultate Lasius platythorax, Lasius niger, Formica cunicularia e Formica pratensis. È stata attuata anche l'analisi biometrica delle singole fatte per definire meglio la grandezza e la correlazione con il numero di prede contenute.
Aim In this paper we investigate the evolutionary history of the Eurasian green woodpecker (Picus viridis) using molecular markers. We specifically focus on the respective roles of Pleistocene ...climatic oscillations and geographical barriers in shaping the current population genetics within this species. In addition, we discuss the validity of current species and subspecies limits. Location Western Palaearctic: Europe to western Russia, and Africa north of the Sahara. Methods We sequenced two mitochondrial genes and five nuclear introns for 17 Eurasian green woodpeckers. Multilocus phylogenetic analyses were conducted using maximum likelihood and Bayesian algorithms. In addition, we sequenced a fragment of the cytochrome b gene (cyt b, 427 bp) and of the Z-linked BRM intron 15 for 113 and 85 individuals, respectively. The latter data set was analysed using population genetic methods. Results Our phylogenetic results support the monophyly of Picus viridis and suggest that this taxon comprises three allopatric/parapatric lineages distributed in North Africa, the Iberian Peninsula and Europe, respectively. The North African lineage split from the Iberian/European clade during the early Pleistocene (1.6-2.2 Ma). The divergence event between the Iberian and the European lineages occurred during the mid-Pleistocene (0.7-1.2 Ma). Our results also support a post-glacial range expansion of these two lineages from distinct refugia located in the Iberian Peninsula and possibly in eastern Europe or Anatolia, which led to the establishment of a secondary contact zone in southern France. Main conclusions Our results emphasize the crucial role of both Pleistocene climatic oscillations and geographical barriers (Strait of Gibraltar, Pyrenees chain) in shaping the current genetic structure of the Eurasian green woodpecker. Our molecular data, in combination with diagnosable plumage characters, suggest that the North African green woodpecker (Levaillant's woodpecker) merits species rank as Picus vaillantii (Malherbe, 1847). The two European lineages could be distinguished by molecular and phenotypic characters over most of their respective geographical ranges, but they locally exchange genes in southern France. Consequently, we prefer to treat them as subspecies (P. viridis viridis, P. viridis sharpei) pending further studies.
In this study, we re-evaluated historical demography of the Eurasian green woodpecker (Picus viridis) on the basis of previously published multi-locus mitochondrial DNA (mtDNA) data and ecological ...niche modelling. We particularly aimed to test glacial refugia hypothesis during climatic oscillations of the late Quaternary for the Eurasian green woodpecker. Our results indicate that the Eurasian green woodpecker was sensitive to the effects of climate change. Prior to the Last Glacial Maximum (22000 years ago), the population contraction started and the Eurasian green woodpecker restricted its range to southern Europe (including France), Anatolia, and the Caucasus/Caspian region, and, afterwards, substantially expanded its range from this restricted area to its present range around 14500 years before present. Therefore, during the Last Glacial Maximum, we recognize a single large refugium for this species, located in southern Europe (including France), Anatolia, and the Caucasus/Caspian region.
This study explores the relative abundance of hole-nesting birds in four Mediterranean forest types, each of which has undergone different patterns of forest management. Nine species were sampled in ...24 study plots, to compare cork oak forest, turkey oak forest, holm oak forest and pine plantation. The abundance of hole-nesters was greater in cork oak forest and turkey oak forest. Three species were most frequently detected: Great Tit (Parusmajor), Blue Tit (Cyanistes caeruleus) and Nuthatch (Sitta europaea). Bird abundancewas significantly lower in holmoak forest, particularly in the cases of Great Spotted Woodpecker (Dendrocopos major), Green Woodpecker (Picus viridis), Nuthatch and Short-toed Treecreeper (Certhia brachydactyla). TheGreat SpottedWoodpecker showed a positive correlation with the abundance of three secondary cavity nesters: Nuthatch, Short-toed Treecreeper and Starling; in contrast, Green Woodpecker showed a negative correlation with Starling. Habitat structure varied significantly among forest types, especially themean andmaximumtree height, these being lower in holmoak forest. The Great SpottedWoodpecker proved to be a good indicator of less disturbed woodlands. In fact, maximum tree height turned out to be a significant and positive explanatory variable for woodpecker abundance.We consider that intensive coppicing and timbermanagement in holm oak forest during the 20th century widely affected trees' age-profile, with consequences for their suitability forwoodpeckers and other hole-nesting birds. Tomonitor the response of hole-nesting birds to forest management in terms of abundance, we propose the use of the great spotted woodpecker as an indicator species.
Secondary contact zones are natural systems which can be efficiently used to measure genetic differentiation and gene flow and thus provide a good opportunity to assess the level of reproductive ...isolation between divergent evolutionary lineages. In this study, we used ten Z-linked and nine autosomal loci from seven chromosomes and twenty males to evaluate gene flow across a secondary contact zone between two mitochondrial lineages of the Eurasian Green Woodpecker (
Picus viridis
), that diverged around 1 million years ago. One lineage (
Picus viridis
sharpei
) is distributed throughout the Iberian Peninsula whereas the other one (
Picus viridis
viridis
) is widespread across the Western Palearctic. These two lineages form a secondary contact zone in southern France. Formerly treated as two subspecies of
Picus viridis
, several authors have recently proposed assigning a specific rank to
P. viridis sharpei
and
P. viridis viridis
. Our results indicate no introgression of nuclear loci in allopatric populations located on both sides of the contact zone, which thus acts as an efficient barrier to gene flow. All males sampled within the contact zone and one male sampled near its eastern border were slightly admixed revealing that reproductive isolation between
P. viridis
sharpei
and
P viridis
viridis
has not been completely achieved. In accordance with the geographical range of each lineage, the two admixed males sampled near the western border of the contact zone harboured a large proportion of
P. viridis
sharpei
alleles whereas admixed males sampled eastwardly near the Rhone Valley had a high proportion of
P. viridis
viridis
alleles. Overall our results further support considering
P. viridis
sharpei
and
P. viridis
viridis
as two biological species.
Dead wood is important for woodpeckers, providing foraging, roost and nest-sites. In this paper, data from long-term studies of woodpeckers and dead wood in oakwoods in southern England are used to ...examine the dead wood requirements of the three British resident woodpecker species. Both Great Dendrocopos major and Lesser Spotted Woodpeckers Dendrocopos minor select dead trees for nest-sites although the former is able to nest in living trees too. On the other hand a smaller fraction of Lesser Spotted Woodpecker nests are in living trees. Green Woodpecker Picus viridis shows no selection for dead nesting trees. Hence the smallest woodpecker species appears to be most dependent on dead and decaying trees for nest-sites. Great and Lesser Spotted Woodpeckers show no preference for foraging on dead trees although they both make use of dead branches on living trees. Lesser Spotted Woodpeckers forage on smaller branches higher in the tree than Great Spotted Woodpeckers. There has been a trend for increasing dead wood resources in the study woods with both dead wood on the ground and standing dead trees (snags) increasing in the last 20 years. The levels of dead wood are shown to be the result of continual processes of creation and decay. Around 0.5% of oak Quercus spp., Ash Fraxinus excelsior and Hornbeam Carpinus betulus and 3.4% of the birch Betula spp. trees die each year in the woods resulting in a continuity of new dead snags and fallen trees. There is a high turnover of standing dead snags of oak and birch with 95% and 80% annual survival, respectively. Snags are only suitable for nesting Great Spotted Woodpeckers for a few years after their creation. It is suggested that these stand and dead wood dynamics are likely to provide habitats more favourable for the Great Spotted than the Lesser Spotted Woodpecker.
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