Cancer cells overexpress IFsub.1, the endogenous protein that inhibits the hydrolytic activity of ATP synthase when mitochondrial membrane potential (Δμsub.H sup.+) falls, as in ischemia. Other roles ...have been ascribed to IFsub.1, but the associated molecular mechanisms are still under debate. We investigated the ability of IFsub.1 to promote survival and proliferation in osteosarcoma and colon carcinoma cells exposed to conditions mimicking ischemia and reperfusion, as occurs in vivo, particularly in solid tumors. IFsub.1-silenced and parental cells were exposed to the FCCP uncoupler to collapse Δμsub.H sup.+ and the bioenergetics of cell models were validated. All the uncoupled cells preserved mitochondrial mass, but the implemented mechanisms differed in IFsub.1-expressing and IFsub.1-silenced cells. Indeed, the membrane potential collapse and the energy charge preservation allowed an increase in both mitophagy and mitochondrial biogenesis in IFsub.1-expressing cells only. Interestingly, the presence of IFsub.1 also conferred a proliferative advantage to cells highly dependent on oxidative phosphorylation when the uncoupler was washed out, mimicking cell re-oxygenation. Overall, our results indicate that IFsub.1, by allowing energy preservation and promoting mitochondrial renewal, can favor proliferation of anoxic cells and tumor growth. Therefore, hindering the action of IFsub.1 may be promising for the therapy of tumors that rely on oxidative phosphorylation for energy production.
A one-of-a-kind presentation of the major achievements in statistical profile monitoring methods Statistical profile monitoring is an area of statistical quality control that is growing in ...significance for researchers and practitioners, specifically because of its range of applicability across various service and manufacturing settings. Comprised of contributions from renowned academicians and practitioners in the field, Statistical Analysis of Profile Monitoring presents the latest state-of-the-art research on the use of control charts to monitor process and product quality profiles. The book presents comprehensive coverage of profile monitoring definitions, techniques, models, and application examples, particularly in various areas of engineering and statistics. The book begins with an introduction to the concept of profile monitoring and its applications in practice. Subsequent chapters explore the fundamental concepts, methods, and issues related to statistical profile monitoring, with topics of coverage including: Simple and multiple linear profiles Binary response profiles Parametric and nonparametric nonlinear profiles Multivariate linear profiles monitoring Statistical process control for geometric specifications Correlation and autocorrelation in profiles Nonparametric profile monitoring Throughout the book, more than two dozen real-world case studies highlight the discussed topics along with innovative examples and applications of profile monitoring. Statistical Analysis of Profile Monitoring is an excellent book for courses on statistical quality control at the graduate level. It also serves as a valuable reference for quality engineers, researchers and anyone who works in monitoring and improving statistical processes.
Six Sigma is a management program that provides tools that help manufacturers obtain efficient, stream-lined production to coincide with ultimate high quality products. Lean Six Sigma will show how ...the well-regarded analytical tools of Six Sigma quality control can be successfully brought into the well-established models of 'lean manufacturing', bringing efficient, stream-lined production and high quality product readily together. This book offers a thorough, yet concise introduction to the essential mathematics of Six Sigma, with solid case examples from a variety of industrial settings, culminating in an extended case study. Various professionals will find this book immensely useful, whether it be the industrial engineer, the industrial manager, or anyone associated with engineering in a technical or managing role. It will bring about a clear understanding of not only how to implement Six Sigma statistical tools, but also how to do so within the bounds of Lean manufacturing scheme. It will show how Lean Six Sigma can help reinforce the notion of 'less is more', while at the same time preserving minimal error rates in final manufactured products.
All providers of laboratory medicine services aim to provide a 'high quality' service but our understanding of what this means is variable. The IFCC has developed a 'quality ladder' to illustrate the ...stages of quality improvement. The aim of all laboratory specialists should be continuous quality improvement, effectively climbing the rungs of the quality ladder. The top of the ladder is reached through laboratory accreditation against the international standard ISO 15189:9019. Continuous quality improvement requires an active programme of quality management. This involves a continuous series of internal audits against locally established quality standards supplemented by benchmarking against external standards of performance. Documentation and document control are essential internal elements of quality management. External standards include: staff training and qualifications; performance in external quality assurance programmes; user satisfaction surveys; and, ultimately, full accreditation by external assessors against ISO 15189:9019. Everyone working in a clinical laboratory should contribute to continuous quality improvement. The results from laboratory medicine investigations inform a high percentage of all clinical decisions. Therefore, provision of a high quality service is of critical importance to patients, both in terms of patient safety and improving quality outcomes. In an increasingly litigious world avoidable quality failures in the provision of laboratory medicine services are unacceptable to patients and the public. Laboratory directors are being asked to provide evidence of their quality standards of performance. Contracts for service provision, clinical trials and research programmes are becoming conditional on provision of that evidence.
ACBI/CMC EQAS--QUO VADIS? Geethanjali, F.S; Selvakumar, R
Indian journal of clinical biochemistry,
05/2022, Letnik:
27, Številka:
S1
Journal Article
Recenzirano
Objective: The objective of this retrospective study is to evaluate the usefulness of the ACBI/CMC EQAS program in the overall performance of Indian Laboratories as well as individual labs in ...different categories over the years. It is the journey of our National EQAS over the past decade. Method: The awareness of the Indian labs towards quality assurance is evaluated based on the number of participants, the response in submitting results, annual evaluation and the trend in methods selection. Similarly few labs form each category such as lab size (number of parameters done), type (public/private) and few labs at random were selected and their performance is evaluated over the past 10 years. Results: The awareness of Quality assurance in India has tremendously increased and is reflected by a 100% increase in the number of participants over the past ten years. The randomly selected labs showed decreased OMVIS which is indirectly proportional to their performance. Conclusion: It was during this past 10 years, lot of changes took place in the ACBI/CMC EQAS, i.e., change over form bovine sera to human sera, liquid samples to lyophilised samples, introducing immunoassay program etc. This study shows how the Indian laboratories have improved their performance as well as their awareness towards the QA-concept. It is very encouraging to note that the concept of EQAS is well accepted and appreciated by majority of laboratories and thus paving the way towards international standards.
PDF
