In this study, 640 cattle and 742 sheep slaughtered at twelve abattoirs in Thrace were investigated for hydatid cysts. The infection rate of hydatidosis was 11.6% in cattle and 3.50% in sheep. The ...cysts were found in 3.15% of 127 calves ( < 1 year old), in 13.6% of 513 cattle (between 1-10 years old), in 2.64% of 720 lambs ( < 1 year old) and in 31.8% of 22 sheep (between 1-6 years old). The infection rate was 23.6% in female cattle and 2.95% in male cattle while the rate was 4.50% in female sheep and 1.82% in male sheep. Out of the infected cattle, 79.7% had cysts in the lungs and 64.9%, in the liver. In infected sheep, cysts were encountered in the livers of 96.2%, in the lungs of 26.9%, and in the spleen of 3.85%. Out of infected cattle, 4.05% had fertile cysts and 23.1% of infected sheep had fertile cysts.
In this study, the polymorphisms in calpain (CAPN1), estrogen receptor α (ERα), prolactin (PRL), and myostatin (MSTN) genes thought to be related to some economical traits were investigated in 136 ...heads from Holstein and Brown Swiss cattle breeds reared in Karacabey town of Bursa by using PCR-RFLP method. While MSTN locus was found as monomorphic, 2 alleles of each CAPN1, ERα and PRL genes were determined in both breeds. While 3 genotypes (CC, CT, and TT) were determined at CAPN1 locus of both breeds, 2 genotypes (AG and GG) and 3 genotypes (AA, AG, and GG) Juszczukwere Determined at PRL locus of Holstein and Brown Swiss breeds, respectively. Two genotypes (AG and GG) were detected at ERα locus in both breeds. Both two populations were found at Hardy-Weinberg equilibrium except for PRL locus in Brown Swiss population.
Cattle manure (CM) is mostly used as an inoculum in order to start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic content. Rumen fluid microbiota ...is also considered to be effective in lignocellulose digestion. It is known that microorganisms in the ruminal waste facilitate the hydrolysis of lignocellulosic structures. However, there are few studies on the inoculum effect of rumen microorganisms on bioreactor performances when rumen content is used as co-substrate and inoculum together with cattle manure, and it is not clear how rumen waste can be used in various anaerobic digestion systems. In this study, biogas production efficiencies obtained from lignocellulosic content in mono and cosubstrate (1:1 and 1:2 Volatile Solids) bioreactors formed by using ruminal waste (RW) and CM microbial communities were investigated. In order to determine the biogas production efficiencies, biomethane potential (BMP) experiments that simulate anaerobic digestion process conditions in a laboratory environment were applied. Treatment with 50% application of RW cosubstrate, 65.51% biogas and 70.64% methane production efficiency increases were achieved compared to monosubstrate CM bioreactor.
To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge ...samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV- 4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.
Bu çalışmanın amacı Türkiye’deki holştayn ineklerde üridin monofosfat senteaz eksikliği (DUMPS) allelinin bulunup bulunmadığının araştırılmasıdır. Bu kalıtsal hastalıktan kaynaklanan ekonomik kaybı ...önlemenin tek yolu DUMPS taşıyıcılarının erken belirlenmesidir. Bu çalışmada, toplam 200 baş dişi holştayn polimeraz zincir reaksiyonu-restriksiyon parçacık uzunluk polimorfizmi (PCR-RFLP) yöntemi ile incelenmiştir. İncelenen dişi holştaynlarda DUMPS taşıyıcısına rastlanmamıştır.
Leptin gene polymorphisms in native Turkish Cattle breeds Öztabak, K., İstanbul Univ., Faculty of Veterinary Medicine, İstanbul (Turkey). Div. of Biochemistry; Toker, N.Y., İstanbul Univ., Faculty of Veterinary Medicine, İstanbul (Turkey). Div. of Biochemistry; Ün, C., Ege University, Faculty of Science, İzmir (Turkey). Div. of Biology ...
Veteriner fakultesi dergisi,
(2010), 2010, Letnik:
16, Številka:
6
Journal Article
Recenzirano
Odprti dostop
The aim of the study was to determine leptin gene polymorphisms in South Anatolian Red (SAR), East Anatolian Red (EAR) and Turkish Grey Cattle. In the study unrelated 40 SAR, 40 EAR and 40 Turkish ...Grey cattle were used. Target sites in leptin gene exon 2, exon 3 and intron 2 were amplified by polymerase chain reaction (PCR). The single nucleotide polymorphism (SNP) consisting site in exon 2, exon 3 and intron 2 were determined as a result of digestion with Kpn2I, HphI and Sau3AI restriction enzymes, respectively. The highest T allele frequency related with production traits for Kpn2I polymorphism was found for SAR cattle. For HphI polymorphism, T allele frequencies were detected clearly predominant. Within each breed for Sau3AI polymorphism B and C allele frequencies that effect production traits were found to be dramatically lower than A allele frequency. As a result we can suggest that there was no clearly difference that can create any advantage in terms of leptin gene SNPs among the three native Turkish cattle breeds.
Bu çalışmada Doğu Anadolu Kırmızısı (DAK), Güney Anadolu Kırmızısı (GAK) ve Boz ırk sığırlarda leptin geni polimorfizmlerinin belirlenmesi amaç edinilmiştir. Çalışmada birbiri ile yakınlığı olmayan 40 adet GAK, 40 adet DAK ve 40 adet Boz ırk elde edilmesinin ardından leptin geni ekson 2, ekson 3 ve intron 2'deki hedef bölgeler polimeraz zincir reaksiyonu (PCR) ile çoğaltılmıştır. Ekson 2'deki tek nükleotid polimorfizmi (SNP) içeren bölge Kpn2I, ekson 3'te SNP içeren bölge HphI ve intron 2'de SNP içeren bölge Sau3AI restriksiyon enzimleri ile sindirilmesi sonucu belirlenmiştir. Kpn2I polimorfizmi için verim özellikleriyle ilişkili T allel frekansı en yüksek GAK ırkı sığırlarda bulunmuştur. Her üç sığır ırkında da HphI polimorfizmi için T allel frekansı oldukça yüksek bulunmuştur. Her ırk içinde Sau3AI polimorfizmi için verim özelliklerini etkileyen B ve C allel frekansları, A allel frekansından dikkat çekici şekilde düşük olduğu bulunmuştur. Sonuç olarak istatistiki açıdan üç sığır ırkı arasında leptin geni SNP'leri açısından avantaj doğurabilecek önemli bir farklılığın olmadığını ileri sürebiliriz.
Bu çalışmada Bursa bölgesinde yetiştirilen İsviçre Esmeri ve Siyah Alaca sığırlarda β-lg ve bGH gen polimorfi zmleri HaeIII ve MspI restriksiyon enzimleri kullanılarak incelenmiştir. İsviçre ...Esmer'lerinde β-lg A ve B allellerinin frekansları sırasıyla 0.3430 ve 0.6570 olarak bulunurken, Siyah Alaca ırkına ait örneklerde sırasıyla 0.5480 ve 0.4520 olarak saptanmıştır. Hem İsviçre Esmeri hem de Siyah Alaca ırklarına ait örneklerde bGH'nun MspI (+) allellinin predominat olduğu belirlenmiştir. İsviçre Esmerlerinde MspI (+) allellinin frekansı 0.9623, Siyah Alacalarda ise 0.8158 olarak hesaplanmıştır. Her iki populasyon, her iki lokus bakımdan Hardy-Weinberg dengesinde bulunmuştur.
In this study polymorphism on β-lg and bGH genes in Holstein and Brown Swiss cattle reared in Bursa region were investigated by using HaeIII and MspI restriction enzymes. While frequencies of A and B allelles of β-lg were found 0.3430 and 0.6570 in Brown Swiss population, they were found as 0.5480 and 0.4520 in Holstein population, respectively. MspI (+) allelle of bGH was predominat in both Brown Swiss and Holstein populations with frequency of 0.9623 and 0.8158, respectively. Both of two populations were found in Hardy- Weinberg equilibrium for both of the two loci investigated.
Kars merkez ve Selim ilçesine bağlı köylerde atık yaptığı bilinen sığır sürülerinde bulunan ve hastalığa karşı aşılanmamış 250 inekten elde edilen süt ve vajinal sıvap örnekleri olmak üzere toplam ...500 örnek Brusella cinsi bakteriler yönünden kültürelolarak değerlendirildi. Bakteriyolojik inceleme sonucunda 250 süt örneğinin 11 (%4.4)'inden ve 250 vajinal sıvap örneğinin 16 (%6.4)'sından olmak üzere toplam 27 (%5.4) örnekten Brucella spp. izolasyonu yapıldı. Bu izolatların, Brusella cinsine (Ompbp26) ve eritritol (ery) genine spesifik primerle yapılan PCR incelemesinde hepsinin Brucella spp. ve saha suşları olduğu görüldü.İzole edilen tüm suşlar B. abortus biyotip 3 olarak tiplendirildi. Ayrıca bu izolatların RAPD-PCR ile incelemesinde elde edilen DNA profillerinin, B. abortus S19 ile homoloji gösterdiği tespit edildi.
In this study, the milk and vaginal swab samples, collected from each of 250 cows raised in Kars and Selim areas, were analysed for culturing Brucella species. These animals had a history of abortion and they were unvaccinated against Brucellosis. Based on the bacteriological examination, Brucella spp. was isolated from total of 27 (%5.4) samples, 11 (%4.4) and 16 (%6.4) originated from milk and vaginal swab samples, respectively. These isolates were found to be Brucella spp. Are field isolates based on the PCR analysis using primers specific to Brucella genus (Omp bp26) and erythritol (ery). All of the isolated strains were typed as B. abortus biotype 3. RAPD-PCR analysis showed that DNA patterns of these isolates were found to be similar to that of B. abortus S19.
Erzurum il merkezi ve çevresinden olmak üzere 12 odaktaki 30 buzağı ahır/çiftliği 2007 yılı Mart-Nisan aylarında ziyaret edilmiştir. Üç aylığa kadar olan buzağıların rektumlarından alınan dışkı ...örnekleri santrifüj edilmiş ve sedimentten yaymalar hazırlanarak modifiye asit fast boyama yöntemi ile incelenmiştir. Erzurum yöresinde buzağılarda Cryptosporidium prevalansı %22,8 (43/189) oranında bulunmuştur. Bu protozoonun prevalansı ishalli buzağılarda %30,3 (36/119), normal dışkılı olanlarda %10.0 (7/70) olarak saptanmıştır. Buzağılarda Cryptosporidium türlerinin odak/yerleşim yeri ve ahır/çiftlik prevalansı sırası ile %91,7 (11/12) ve %53,3 (16/30) olarak tespit edilmiştir. Enfeksiyonun prevalansı devlet çiftliklerindeki buzağılarda %17,3 (18/104) olduğu halde, bu oran köy ahır/çiftliklerinde %29,4 (25/85)'e kadar çıkmıştır. Araştırma süresince iki çiftlikte (2/30, %6,7) ve 16 (16/189; %8,5) ishalli buzağıda klinik ön tanı olarak cryptosporidiosisden şüphelenilmiştir. Halka ait olan bu çiftliklerdeki buzağıların %87,5 (14/16)'inde Cryptosporidium sp. ookistleri saptanmıştır.
This study was carried out between March and April, 2007 to determine the prevalence of Cryptosporidium spp. in calves. The study was performed in 30 farms of 12 different localities of Erzurum and vicinity. The fecal samples were taken directly from the rectums of calves which were no more than three months old. The samples were centrifuged and examined under microscope by using the modified acid-fast technique. The prevalence of Cryptosporidium spp. was found to be 22.8% (43/189). The rate of infection was 30.3% (36/119) in calves with diarrhea and 10% (7/70) in healthy calves. The prevalence of the protozoan was 91.7% (11/12) and 53.3% (16/30) in localities and farms respectively. The prevalence was 17.3% (18/104) in state farms and 29.4% in village farms. During the research period, the presence of Cryptosporidiosis was suspected in 16 calves on two different farms. Fourteen of 16 calves (87.5%) were found to be infected with Cryptosporidium spp.
Levels of tetracycline residues in cattle meat, liver, and kidney from a slaughterhouse in Tabriz, Iran Abasi, M.M., Tabriz University of Medical Sciences, Drug Applied Research Center, Tabriz (Iran); Rashidi, M.R., Tabriz University of Medical Sciences, Drug Applied Research Center, Tabriz (Iran); Javadi, A., Islamic Azad University, Faculty of Veterinary Medicine, Tabriz (Iran). Div. of Food Hygiene ...
Turkish Journal of Veterinary & Animal Sciences,
(2009), 2009, Letnik:
33, Številka:
4
Journal Article
Recenzirano
Odprti dostop
Total of 500 samples from triceps, gluteal and diaphragm muscles, kidney, and liver, were obtained randomly from beef carcasses of a slaughterhouse in Tabriz. We measured oxytetracycline, ...tetracycline, and chlortetracycline (TCs) residues by high performance liquid chromatography (HPLC) method. Seventy four percent of samples (380 samples) had detectable TCs residues. The mean amounts of oxytetracycline, tetracycline, and chlortetracycline residues in all samples were 52.2, 33.8, and 125.2 µg/kg, respectively. The mean amounts of total residues of TCs in all meat samples, triceps, gluteal and diaphragm muscles, kidney, and liver were 131.0, 163.1, 63.4, 166.7, 408.1, and 254.9 µg/kg, respectively. Five percent of kidney and liver samples and 21.7% of all samples contained residues more than the maximum residue limits (MRLs) of the World Health Organization (WHO). This study indicates presence of different levels of tetracycline residues in the various edible tissues. Regulatory authorities should ensure proper withdrawal period before slaughtering of the animals.
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