The forgotten future of the phage Woolfson, Adrian
Science (American Association for the Advancement of Science),
08/2023, Letnik:
381, Številka:
6658
Journal Article
Recenzirano
As antibiotic resistance soars, a journalist revisits an often overlooked antimicrobial strategy
Imipenem-relebactam (IPR) has been reported to exhibit a good activity against non-metallo-ß-lactamase carbapenem-resistant Pseudomonas aeruginosa (CRPA), and the rapid detection of ...susceptibility/resistance to this new therapeutic alternative may be crucial. Therefore, the Rapid IPR Pseudomonas NP test was developed to quickly identify IPR susceptibility/resistance among multidrug-resistant P. aeruginosa.
The principle of the Rapid IPR Pseudomonas NP test is based on visually detecting glucose metabolization by observing (or not) a color change from yellow to red or orange of the red phenol pH indicator in the presence of imipenem at 2 mg/L and relebactam at 4 mg/LA total of 80 clinical Pseudomonas aeruginosa isolates were analyzed, among which 42 isolates were IPR resistant according to EUCAST guidelines (MICs, susceptible ≤ 2 mg/L, resistant > 2 mg/L). Results obtained with the Rapid IPR Pseudomonas NP test were compared with the reference broth microdilution (BMD).
The sensitivity, specificity and accuracy of the test were found to be 100%, 89.5% and 95%, respectively, using the BMD reference method as a comparator. Moreover, five out of the IPR-susceptible isolates (n=38) exhibiting an MIC of IPR close to the breakpoint (MIC = 1 mg/L, n=2; MIC=2mg/L, n=3) yielded to a major error result, namely a positive result with the rapid IPR Pseudomonas NP test (resistance). By contrast, all IPR-resistant isolates (n=42) were all correctly categorized.
The Rapid IPR Pseudomonas NP test is sensitive, specific, and easy to perform and interpret. Therefore, it is suitable for implementation in routine clinical laboratories.
Infectious diseases are the second most important cause of human death worldwide;
is a very common human pathogenic microorganism that can trigger a variety of infectious diseases, such as skin and ...soft tissue infections, endocarditis, osteomyelitis, bacteremia, and lethal pneumonia. Moreover, according to the sensitivity to antibiotic drugs,
can be divided into methicillin-sensitive
(MSSA) and methicillin-resistant
(MRSA). In recent decades, due to the evolution of bacteria and the abuse of antibiotics, the drug resistance of
has gradually increased, the infection rate of MRSA has increased worldwide, and the clinical anti-infective treatment for MRSA has become more difficult. Accumulating evidence has demonstrated that the resistance mechanisms of
are very complex, especially for MRSA, which is resistant to many kinds of antibiotics. Therefore, understanding the drug resistance of MRSA in a timely manner and elucidating its drug resistance mechanism at the molecular level are of great significance for the treatment of
infection. A large number of researchers believe that analyzing the molecular characteristics of
can help provide a basis for designing effective prevention and treatment measures against hospital infections caused by
and further monitor the evolution of
. This paper reviews the research status of MSSA and MRSA, the detailed mechanisms of the intrinsic antibiotic resistance and the acquired antibiotic resistance, the advanced research on anti-MRSA antibiotics and novel therapeutic strategies for MRSA treatment.
The main objective of this study was to investigate the antibiotic resistance (AR) levels in wastewater (WW) and the impact on the receiving river. Samples were collected once per season over one ...year in the WW of a hospital, in the raw and treated WW of two wastewater treatment plants (WWTPs), as well as upstream and downstream from the release of WWTPs effluents into the Zenne River (Belgium). Culture-dependent methods were used to quantify Escherichia coli and heterotrophic bacteria resistant to amoxicillin, sulfamethoxazole, nalidixic acid and tetracycline. Six antibiotic resistance genes (ARGs) were quantified in both particle-attached (PAB) and free-living (FLB) bacteria. Our results showed that WWTPs efficiently removed antibiotic resistant bacteria (ARB) regardless of its AR profile. The ARGs levels were the highest in the hospital WW and were significantly reduced in both WWTPs. However, ARB and ARGs abundances significantly increased into the Zenne River downstream from the WWTPs outfalls. The variation in the relative abundance of ARGs through WW treatment differed depending on the WWTP, fraction, and gene considered. The sul1 and sul2 genes in PAB fraction showed significantly higher relative abundances in the effluent compared to the influent of both WWTPs. This study demonstrated that WWTPs could be hotspots for AR spread with significant impacts on receiving freshwater ecosystems. This was the first comprehensive study investigating at the same time antibiotics occurrence, fecal bacteria indicators, heterotrophic bacterial communities, and ARGs (distinguishing PAB and FLB) to assess AR levels in WW and impacts on the receiving river.
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•Brussels WWTPs efficiently remove antibiotic resistant bacteria (ARB).•Significant increase of ARB was found downstream from the WWTPs outfalls.•Absolute ARGs abundances are reduced from influents to effluents of both WWTPs.•Significant increase of ARGs was found downstream from the WWTPs outfalls.•Some ARGs relative abundance significantly increased in the effluent of WWTPs.
The ubiquity of antibiotic-resistance bacteria (ARB) and antibiotic-resistance genes (ARGs) in various environmental matrices is a potential threat to human and ecological health. Therefore, the ...inactivation of ARB E. coli S1-23 and the elimination of its associated ARGs, blaTEM-1 and aac(3)-II, were investigated using the photoelectrocatalytic (PEC) process. Results indicate that the ARB E. coli S1-23 (1 × 108 cfu mL−1) and its ARGs (extracellular and intracellular) could be fully inactivated within 10 and 16 h PEC treatment, respectively. In contrast, photocatalytic (PC) and electrochemical (EC) treatments displayed no obvious effect; however, ARG-containing DNA extracted from E. coli S1-23, which was used as a model for dissociative naked ARGs, could be completely decomposed within a few minutes through these three treatments. Further analyses, including PCR, AFM and HPLC, proved that the structural integrity and surface topography of naked ARGs are damaged during treatment and can be completely eliminated. Furthermore, there is no generation of cytosine, guanine, adenine or thymine intermediates during the PEC, PC, and EC treatments. This study is the first report to propose the PEC treatment as a promising method for complete decomposition of ARB and ARGs in aqueous systems.
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•PEC treatment can effectively inactivate ARB and intracellular ARGs can leak out.•Leaked ARGs can be fully destroyed with prolonging treatment time after killing ARB.•Dissociative naked ARG-containing DNA could also be fully destroyed by PEC treatment.
This Special Issue on Antimicrobial Resistance in Environmental Waters features 11 articles on the monitoring and surveillance of antimicrobial resistance (AMR) in natural aquatic systems (i.e., ...reservoirs, rivers), and effluent discharge from water treatment plants to assess the effectiveness of AMR removal and resulting loads in treated waters. Some of the key elements of AMR studies presented in this Special Issue highlight the underlying drivers of AMR contamination in the environment and the evaluation of the hazard imposed on aquatic organisms in receiving environments through ecological risk assessments. As described in this Issue, screening antimicrobial peptide (AMP) libraries for biofilm disruption and antimicrobial candidates are promising avenues for the development of new treatment options to eradicate resistance.
Aquatic environments are identified as an ideal setting for acquisition and dissemination of antibiotic resistance, and human exposure to antibiotic resistant bacteria (ARB) and antibiotic resistance ...genes (ARGs) in aquatic environments may pose an additional health risk. Quantitative microbial risk assessment (QMRA) has been suggested as a suitable method to evaluate and quantify this health risk. However, information about the exposure to ARB and ARGs in aquatic environments is lacking for many scenarios and dose-response models regarding the ARB infections are not developed yet. This review summarizes the current knowledge regarding the ARB and ARGs in aquatic environments and highlights the challenging questions remaining to be answered to better forecast the health risks caused by ARB and ARGs in water environments. The questions include what are the missing information needed to quantify the human health risks caused by exposing to ARB and ARGs in aquatic environments? what are the suitable markers to evaluate the ARB/ARGs contamination in aquatic environments? how frequently do the ARG selection and propagation occur in aquatic environments? and are there any unknown hot spots? Studies on the above topics will contribute to better management of antibiotic resistance dissemination in water environments and its risks on human health.
Abbreviations
3GC
3
rd
generation cephalosporins
ARB
Antibiotic resistant bacteria
ARG
Antibiotic resistance gene
CFU
Colony forming unit
DBP
Disinfection by-products
eDNA
Extracellular DNA
EPS
Extracellular polymeric substances
HGT
Horizontal gene transfer
ISCR
Insertion sequence common region
MAR
Multiple antibiotic resistant
MIC
Minimum inhibitory concentration
MGE
Mobile genetic elements
MSW
Municipal solid waste
QMRA
Quantitative microbial risk assessment
VBNC
Viable but non-culturable
WWTP
Wastewater treatment plant
The growing antibiotic resistance of microorganisms is an increasingly important challenge for modern medicine. In the next few decades, the so-called post antibiotic era is likely to begin. For this ...reason, the search for new solutions using PACT seems particularly promising.
Four new chlorin compounds were tested and photochemically characterized for singlet oxygen generation efficiency and photostability. They have been evaluated for bactericidal properties against MRSA, E. coli (ESBL+) and C. albicans. Their synergism with antibiotics from the group of cephalosporins and carbapenems was evaluated.
A reduction of approximately 3 logs or more was achieved for each of the bacterial strains. A decrease in MIC values was observed for all bacteria, the greatest for ceftriaxone.
Combining traditional antibiotic therapy with PACT may be a promising route in the case of antibiotic-resistant infections, but requires further in-depth research.
Swine wastewater treatment plant has become one of the main sources of antibiotic resistance genes (ARGs). Membrane treatment processes are promising solutions for removal of the emerging ...contaminants. However, limited studies have investigated the effects of nanofiltration and reverse osmosis treatment in removing ARGs in swine wastewater. In this study, the presence and the fate of common ARGs including sul1, sul2, tetA, tetM and tetW, as well as intI1 and 16S rRNA gene, were investigated in a medium-sized (6500) pig farm wastewater treatment plant (WWTP) equipped with conventional biological treatment and advanced membrane processing system. All of the genes were detected with highly abundance in the raw sewage. The biological treatments of the swine wastewater treatment plant did not reduce the quantity of the ARGs. As expected, nanofiltration and reverse osmosis treatment reduced the absolute gene copy number of ARGs efficiently (4.98–9.52 logs removal compared to raw sewage). Compared to the reverse osmosis effluent, however, the absolute abundance of ARGs in the artificial wetland increased by 1.00–2.06 logs. Meanwhile, the relative abundance of sulfonamide resistant genes were basically unchanged, while tetracycline resistance genes (tetA, tetM and tetW) decreased by 0.88, 3.47, 2.51 log, respectively. The results demonstrated that advanced membrane treatments are capable of removing various kinds of ARGs efficiently, as well as some common nitrogen and phosphorus contaminants. This study suggested a mature alternative method for the removal of ARGs from livestock wastewater.
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•Swine sewage presents high content of ARGs.•Nanofiltration and reverse osmosis reduces ARGs, nitrogen and phosphorus contaminants.•The abundance of ARGs in artificial wetland increased.