In this paper, for the first time, it is studied the synergetic properties of two different grades of nanocelluloses with different chemical compositions (cellulose nanofibrils-CNF with less than 1% ...of lignin and lignocellulose nanofibrils-LCNF with 16% of lignin). CNF and LCNF were mixed in different ratios to obtain bi-component films. Their performance in terms of transparency, bioactivity, thermo-mechanical and gas barrier properties was evaluated and compared with the performance of the neat CNF films. The presence of LCNF in the formulations conferred antioxidant and UV blocking properties to the films, as well as improved mechanical and barrier properties. Specifically, the incorporation of 25% LCNF to the CNF films increased the mechanical properties (94% increase in tensile stress and a 414% increase in strain at break) and decreased the water vapor transmission rate by 16% and the oxygen transmission rate by 53%. This performance improvement was attributed to the coexistence of nanocelluloses with different chemical composition and morphology. LCNF contributed to increment the interfacial adhesion between cellulose nanofibrils due to the presence of lignin and promote the creation of more tortuous paths for gas molecules. These synergetic properties shown by the CNF/LCNF bi-component films demonstrate high potential to be used as gas barrier packaging solutions.
•CNF and LCNF from residual biomass are used to produce bicomponent nanocellulose films.•LCNF conferred UV-blocking and antioxidant properties to the films.•LCNF presence improved flexibility and tensile strength of CNF films.•All developed bicomponent films display better gas barrier properties than CNF films.•CNF/LCNF formulations have higher potential to be used as barrier coatings/films than CNF.
•To synthesis the cerium oxide nanoparticles using different sources.•Biomedical applications of cerium oxide nanoparticles.•Applications of cerium oxide nanoparticles in toxicity studies.
A cerium ...oxide nanoparticles (nanoceria) has a wide range of applications in different fields, especially biomedical division. As a matter of concern, it has a major impact on the human health and environment. The aim of this review is to address the different ways of synthesis of nanoceria using chemical and green synthesis methods and characterization and the applications of nanoceria for antioxidant, anticancer, antibacterial activities and toxicological studies including the most recent studies carried out in vivo and in vitro to study the problems. We have exclusively discussed on the toxicology of nanoceria exposed to the general public along with recent advances in the studies of antimicrobial, toxicity and anti-oxidant activity.
Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety ...Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS•+ DPPH• and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.
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•Seventy five phenolic compounds were quantified in grape pomace of Prokupac variety.•Considerable amount of ethyl gallate was observed in the grape pomace extracts.•The stem contained significant amount of stilbenoids.•The seed and whole pomace showed the highest radicals and H2O2 scavenging activities.•The extracts of grape pomace skin exerted the cellular antioxidant activity.
•A series of new hydrazones were synthesized with good yield.•Synthesized compounds were evaluated for antibacterial, anti-inflammatory, and anti-oxidant activities.•Upon comparing the synthesized ...molecules with the commonly prescribed medication Diclofenac sodium, we observed a significant enhancement in anti-inflammatory efficacy.•The synthesized compounds demonstrated a notable inclination for DPPH scavenging in the antioxidant assessment, further highlighting their potential in combating oxidative stress.•In silico molecular docking and dynamic investigations were performed to better understand the binding mechanism of the synthesized hydrazones with the target.
Hydrazones, which are azomethine-containing active chemicals with a N(H)NCH group, are extensively researched due to their versatility in pharmacology and ease of synthesis. The synthesized hydrazone compounds were examined by FTIR, UV–visible, EI-MS, 1H NMR, and 13C NMR spectroscopy, and they possessed azomethine linkages. Using the disc diffusion process and minimum inhibitory concentration (MIC) techniques, the compounds were tested for their antibacterial activity. By using the denaturation of bovine serum albumin technique to the synthesized compounds, their anti-inflammatory properties were further examined. Comparing the synthesized molecules to the common medication diclofenac sodium, considerable anti-inflammatory efficacy was observed. Synthesized compounds' antioxidant results revealed a remarkable tendency for DPPH scavenging. Further in silico molecular docking and dynamic studies were evaluated to understand the binding mechanism of the synthesized hydrazones with the target.
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Cellular Antioxidant Activity of Common Fruits Wolfe, Kelly L; Kang, Xinmei; He, Xiangjiu ...
Journal of agricultural and food chemistry,
09/2008, Letnik:
56, Številka:
18
Journal Article
Recenzirano
Measurement of antioxidant activity using biologically relevant assays is important in the screening of fruits for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies ...antioxidant activity in cell culture and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures. The objective of the study was to determine the cellular antioxidant activity, total phenolic contents, and oxygen radical absorbance capacity (ORAC) values of 25 fruits commonly consumed in the United States. Pomegranate and berries (wild blueberry, blackberry, raspberry, and blueberry) had the highest CAA values, whereas banana and melons had the lowest. Apples were found to be the largest contributors of fruit phenolics to the American diet, and apple and strawberries were the biggest suppliers of cellular antioxidant activity. Increasing fruit consumption is a logical strategy to increase antioxidant intake and decrease oxidative stress and may lead to reduced risk of cancer.
C50 carotenoids are promising bioactive compounds produced by halophilic archaea. Relevant studies have focused on their outstanding antioxidant activity, whereas the extraction process has received ...less attention. In this study, the effect of factors on carotenoid extraction efficiency was explored through single-factor and orthogonal experiments. The results showed that the C50 carotenoids were effectively extracted by suspending and homogenizing cell pellets in methanol (1:30, w-v) for 10 min, subsequently incubating at 30 °C for 30 min in the dark, finally centrifuging and drying over vacuum rotary evaporation at 30 °C in the dark. The carotenoid content increased 1.84 times by the above procedure than that before optimization. Moreover, the UVVis spectra, TLC and HPLCMS analysis revealed that the main component of carotenoids extracted from Halorubrum sp. HRM-150 was bacterioruberin (84.12 %), followed by monoanhydrobacterioruberin (15.13 %). Both carotenoid extract and bacterioruberin exhibited antioxidant capacity, which were significantly higher than that of astaxanthin (p < 0.05) by scavenging assays of 2,2-azinobis-3-ethyl-benzothiazole-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals. In addition, the hydroxyl radical scavenging rate of bacterioruberin was significantly higher than that of carotenoid extract (p < 0.01), indicating its potential activity against or delaying diseases caused by reactive oxygen species. C50 carotenoids (especially BR) have great application prospects in human health due to their excellent antioxidant capacity.
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•Bacterioruberin (BR) was the predominant carotenoid in Halorubrum, accounting for 84.12 %.•The carotenoid content was enhanced 1.84 times after extraction optimization.•C50 carotenoid extract displayed higher antioxidant activity than astaxanthin.•BR exhibited higher scavenging rates of·OH radicals than carotenoid extract and astaxanthin.
•Essential oils are a mixture of bioactive volatile compounds.•Essential oils are potent antimicrobial and antioxidant agents.•Essential oils have the potential to be used as a food ...preservative.•There are limitations to the use of essential oils as food preservative.
Essential oils (EOs) are natural, volatile and aromatic liquids extracted from special plants. EOs are complex mixture of secondary metabolites (terpenes, phenolic compounds, alcohol). EOs possess a wide range of biological activities including antioxidant, antimicrobial and anti-inflammatory ones. Particularly, EOs exhibit pronounced antibacterial and food preservative properties that represent a real potential for the food industry. Numerous EOs have the potential to be used as a food preservative for meat and meat products, vegetables and fruits as well as for dairy products. The main obstacles for using EOs as food preservatives are their safety limits, marked organoleptic effects and possible contamination by chemical products such as pesticides. This review aims to provide an overview of current knowledge about EOs food preservative properties with special emphasis on their antibacterial activities and to support their uses as natural, eco-friendly, safe and easily biodegradable agents for food preservation.
•Phenolics were extracted from MOLs using customised deep eutectic solvents (DES).•DES-based ultrasonic-assisted extraction (UAE) conditions were optimized by RSM.•DES-based UAE exhibited high ...efficiency for the extraction of the phenolic compounds.•DES-based UAE showed strong antioxidant activities.•Phenolic constituents in the MOLs extracts were analyzed.
In this study, an ultrasonic-assisted extraction (UAE) procedure with selected deep eutectic solvents (DES) as solvent was first designed to simultaneously optimize the total phenolic/flavonoid content (TPC/TFC) and antioxidant activities of Moringa oleifera L. leaves (MOLs) by using response surface methodology (RSM). The key factors for RSM were selected based on the design of the experimental results along with a three-factors-five-level, central composite design (CCD), including 20 experimental runs. The analysis of variance (ANOVA) results revealed that the water content in DES had a significant influence on all responses, while the ultrasonic time and the ratio of liquid to solid had no statistically significant effects on the total phenolic content. The optimal conditions of the combination of TPC/TFC and antioxidant activities were obtained as follows: 37% water content in DES, 144 W ultrasonic power, and 40 °C ultrasonic temperature. The measured parameters corresponded with the predicted results. Moreover, a comparative study confirmed that the optimized DES-based UAE yielded further higher TPC, TFC, and antioxidant activities than other extraction methods. The results of HPLC analysis in optimized conditions verified that the MOLs extracts with DES-based UAE included 14 phenolic compounds with high concentrations of vicenin-2 (17.6 mg/g) and orientin (23.6 mg/g). The present study supplied a green and high-efficient method for extracting high levels of anti-oxidative phenolic compounds from MOLs.
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