Guavinoside B (GUB) is a characteristic constituent from guava with strong antioxidant activity; however, its low water solubility limits its utilization. Herein, we investigated the interaction ...between GUB and zein, a prolamin with self-assembling property, using multiple spectroscopic methods and fabricated GUB-zein-NaCas nanoparticles (GUB-Z-N NPs) via the antisolvent coprecipitation approach. GUB caused fluorescence quenching to zein via the static quenching mechanism. Fourier-transform infrared spectroscopy and computational analysis revealed that GUB bound to zein via van der Waals interaction, hydrogen bond, and hydrophobic forces. The GUB-Z-N NPs were in the nanometric size range (< 200 nm) and exhibited promising encapsulation efficiency and redispersibility after freeze-drying. These particles remained stable for up to 31 days at 4 °C and great resistance to salt and pH variation, and displayed superior antioxidant activity to native GUB. The current study highlights the potential of zein-based nanoparticles as delivery vehicles for GUB in the food industry.
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•GUB binds to zein mainly via the van der Waals interaction and hydrogen bond.•GUB caused fluorescence quenching to zein via a static quenching mechanism.•GUB-zein-NaCas NPs are fabricated via the anti-solvent coprecipitation method.•GUB-zein-NaCas NPs have good redispersibility and physical and storage stability.•GUB-loaded NPs exhibit superior antioxidant activity than native GUB.
Xylooligosaccharides (XOs) have shown high potential as prebiotics with nutritional and health benefits. In this work, XOs were obtained from highly purified, carboxy-reduced glucuronoarabinoxylans ...by treatment with Driselase®. The mixtures were fractionated, and the structures were elucidated by methylation analysis and NMR spectroscopy. Antioxidant activity was determined by the methods of DPPH and β-carotene/linoleic acid. It was found that the most active oligosaccharides (P3 and G3) comprised 4 or 5 xylose units, plus two arabinoses and one 4-O-methylglucose as side chains, their sequence of units was determined. The optimal concentration for their use as antioxidants was 2 mg/mL. The synthetic antioxidant butylated hydroxytoluene (BHT, 0.2 mg/mL) showed a percentage of inhibition 15% higher than P3. Although its concentration was ∼10 times higher, P3 is non-toxic, and could have great advantages as food additive. These results show that pure XOs exert significant antioxidant activity, only due to their carbohydrate nature.
•XOs were obtained from reduced glucuronoarabinoxylans by treatment with Driselase®.•The XOs mixture was fractionated by GPC on BioGel P-2 to give pure XOs.•Antioxidant activity was measured by DPPH and β-carotene/linoleic acid.•The most active XOs had 4 or 5 xylose units, arabinose, and 4-O-methylglucose.
Apple juice (AJ) was fermented by six select lactic acid bacteria (LAB), namely Lactobacillus plantarum 90 (Lp90), Lactobacillus helveticus 76 (Lh76), Lactobacillus casei 37 (Lc37), Lactobacillus ...paracasei 01 (Lpc01), Lactobacillus acidophilus 85 (La85) and Bifidobacterium lactis 80 (Bla80). The effects of LAB fermentation on sugars and organic acids contents, phenolic profiles, antioxidant capacity and flavor volatiles of fermented AJ (FAJ) were investigated. Results showed that AJ was an excellent food matrix for LAB strains. Lh76 and Lp90 showed the highest viable counts of 12.7 log CFU/mL at the end of fermentation. Lp90, La85 and Bla80 exhibited strong malolactic conversion capacity and finally produced lactic acid more than 6.5 mg/mL. In addtion, LAB fermentation significantly (p < 0.05) decreased the total phenols and flavonoids levels. However, antioxidant activities based on DPPH and FRAP methods were enhanced during fermentation, especially for La85 which exhibited dramatical DPPH radical scavenging activity (90.05%) and FRAP (0.96 mmol Trolox/L). The improvement of antioxidant activities was suggested to be related to the increase in caffeic acid and phlorizin contents. The analysis of flavor volatiles showed that LAB fermentation resulted in formation of seven new alcohols and six new esters, and improvement of ketones and aldehydes production.
•Fuji apple juice was an excellent food matrix for select lactic acid bacteria.•Select lactic acid bacteria exhibited strong malolactic acid conversion capacity.•Antioxidant capacity of apple juice was increased by lactic acid fermentation.•Caffeic acid and phlorizin contents were increased by lactic acid fermentation.•Lactic acid fermentation improved the aroma complexity of apple juice.
In the present study, phytofabricated selenium nanoparticles (PF-SeNPs) were prepared from aqueous fruit extract of
in a facile, green, economic, tactic and eco-friendly way. The aqueous fruit ...extract of
was found to be rich with various secondary metabolites including phenolics (59.18 ± 2.91 mg gallic acid equivalents/g), flavonoids (38.50 ± 2.84 mg catechin equivalents/g), and tannins (44.28 ± 3.09 mg tannic acid equivalents/g) and determined that highly appropriate for the biosynthesis of nanoparticles. The facile phytofabrication of PF-SeNPs was confirmed by UV-visible and FTIR spectroscopic analysis. The XRD pattern and Raman spectroscopy showed that synthesized PF-SeNPs were amorphous in nature. The Zeta potential analysis confirmed that PF-SeNPs were negatively charged (-24.4 mV). The DLS analysis revealed that PF-SeNPs were in nano size and less aggregated with poly-dispersity index of less than 0.2. The SEM images depicted that PF-SeNPs were spherical in shape. The EDX analysis revealed that PF-SeNPs were constituted with Se (61.60%), C (29.96%), and O (4.41%). The HR-TEM analysis determined that PF-SeNPs were in nano size with an average diameter of 15-40 nm. The PF-SeNPs have offered fascinating bio-potential applications, such as antioxidant, antimicrobial and biocompatibility. They have also exhibited dose-dependent free radical scavenging activity, and EC50 was determined as 15.67 ± 1.41 and 18.84 ± 1.02 μg/mL for DPPH and ABTS assays, respectively. The PF-SeNPs has also shown the wide range of antimicrobial activity on foodborne pathogens, and it was found to be highly efficient on fungi followed by Gram-positive and Gram-negative bacteria. The biocompatibility of PF-SeNPs was assessed in N2a cells with much higher IC50 value (dose required to inhibit 50% of cell viability) compared to sodium selenite. Also, mitochondrial membrane potential (MMP) and caspase-3 were much less altered on treatment of PF-SeNPs related to sodium selenite. The cytotoxic studies clearly determined that PF-SeNPs was much less toxic and safer related to sodium selenite. Thus, PF-SeNPs could find suitable application as antioxidant and antimicrobial agent in food, biomedical, and pharmaceutical industry.
Polyphenols as natural antioxidants in cosmetics applications Cherubim, Débora Jackeline; Martins, Cleide Viviane; Fariña, Luciana ...
Journal of cosmetic dermatology,
January 2020, 2020-Jan, 2020-01-00, 20200101, Letnik:
19, Številka:
1
Journal Article
Recenzirano
Background
Currently, there is a great interest in cosmetics prepared on natural resources bases and this may restrict the use of synthetic substances. Plants play a relevant role as a source of ...biologically active natural products with cosmetic and dermatological importance. According to this context, polyphenolic extracts are highlighted because they have proven antioxidant, anti‐inflammatory, anti‐aging, antimicrobial, and supporting activity in solar photoprotection.
Aims
The purpose this study were reviewed at reporting the antioxidant activity of phenolic compounds, mainly applied to dermatological therapy, and highlighting the action mechanisms and structure‐activity relationship.
Methodology
In September 2017, we performed a literature search in PubMed and Scielo for scientific researches, antioxidant studies, and systemic reviews. The search terms we used were “PHYTOCOSMETICS” AND “ANTIOXIDANT ACTIVITY” OR “PHENOLIC COMPOUNDS” (from 2000). As inclusion criteria were used relevant original articles, scientific research in the area of interest, and crucial reference articles. Exclusion criteria were: duplicate publications, non‐relevant articles and not published in English.
Results
The potential cosmetic application of phenolic compounds as natural antioxidants has been attributed to the chemical structure of these compounds, which to interfere in different phases of the oxidation mechanism.
Conclusion
The use of phenolic extracts emerges as a viable alternative for cosmetic application, ensuring a commitment to sustainability. However, it is of crucial importance to evaluate the toxicity risks of raw materials and finished products.
An ultrasound-assisted alkali method was developed to obtain high extraction level of pea protein isolate (PPI). The method parameters of ultrasound-assisted alkali extraction were successfully ...optimized using response surface methodology. The highest extraction level of PPI reached 82.6% at a solid/liquid ratio of 1:11.5 g:mL, pH 9.6, 13.5 min extraction time and 33.7% ultrasonic amplitude. Ultrasound caused changes to the secondary and tertiary protein structure of PPI, resulting in partial protein unfolding and the exposure of hydrophobic groups. In addition, smaller particle size and better dispersion were obtained in PPI with ultrasound-assisted extraction. The ultrasound-assisted alkali method resulted in significant improvements in the functional properties of PPIs, including increased solubility, water/oil holding capacity, foaming/emulsifying capacity, stability, and gel formation capacity. The biological activities of PPIs were also enhanced. Hydroxyl radical scavenging capacity was doubled, and angiotensin-converting enzyme inhibitory activity was increased by 55.1% compared with those of PPI extracted using traditional method. This study provides a potential strategy for the production of pea protein with high efficiency and high quality.
•Higher extraction level of pea protein was achieved with shortened time by US-extraction.•Ultrasound induced changes in secondary and tertiary structure of pea protein.•Functional properties and biological activities were increased by US-extraction.
Milk serves as an important dietary source of bioactive peptides, offering notable benefits to individuals. Among the antioxidant short peptides (di- and tripeptides) generated from gastrointestinal ...digestion are characterized by enhanced bioavailability and bioaccessibility, while assessing them individually presents a labor-intensive and expensive challenge. Based on 4 distinct types of amino acid descriptors (physicochemical, 3D structural, quantum, and topological attributes) and genetic algorithms for feature selection, 1 and 4 machine learning predicted models separately for di- and tripeptides with ABTS radical scavenging capacity exhibited excellent fitting and prediction ability with random forest regression as machine learning algorithm. Intriguingly, the electronic properties of N-terminal amino acid were considered as only factor affecting the antioxidant capacity of dipeptides containing both tyrosine and tryptophan. Four peptides from the potential di- and tripeptides exhibited highly predicted values by the constructed predicted models. Subsequently, a total of 45 dipeptides and 52 tripeptides were screened by a customized workflow in goat milk during in vitro simulated digestion. In addition to 5 known antioxidant dipeptides, 9 peptides were quantified during digestion, falling within the range of 0.04 to 1.78 mg L−1. Particularly noteworthy was the promising in vivo functionality of antioxidant dipeptides with N-terminal tyrosine, supported by in silico assays. Overall, this investigation explored crucial molecular properties influencing antioxidant short peptides and high-throughput screening potential peptides with antioxidant activity from goat milk aided by machine learning, thereby facilitating the identification of novel bioactive peptides from milk-derived proteins and paving the way for understanding their metabolites during digestion.
In this study, alkali-soluble polysaccharide from Manilkara zapota (MZAP) was extracted and polysaccharide-iron complexes MZAP-Fe (III) was synthesized. Structural characterization, antioxidant and ...digestive properties were used to evaluate the feasibility of MZAP-Fe (III) as an iron supplement. The results showed that when the reaction temperature was 70 ℃, the pH was 9 and the addition of sodium citrate was 0.025 g, MZAP- Fe (Ⅲ) had the highest iron content (14.68 %) and its average molecular weight increased compared to MZAP. The UV and FT-IR results indicated that MZAP- Fe (Ⅲ) was formed by the carboxyl and hydroxyl groups in MZAP with the iron group to form Fe-O bonds. MZAP-Fe (III) maintained high thermal stability over the temperature range of 20°C to 450℃. When the concentration of MZAP- Fe (Ⅲ) was 1.0 mg/mL, hydroxyl radical-scavenging activity and DPPH radical scavenging activity reached 57.03 % and 82.21 %, respectively, and both were significantly higher than the antioxidant activity of MZAP. Moreover, in the simulating human digestion, MZAP- Fe (Ⅲ) was more stable than FeSO4 and less likely to be destroyed by stomach acid. The present study demonstrated the potential of MZAP-Fe (III) as a novel iron supplement.
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•MZAP-Fe(Ⅲ) was successfully synthesized with MZAP.•MZAP-Fe (III) possessed superior antioxidant activity than MZAP.•MZAP-Fe (III) could be better utilized in simulated in vitro digestion.
Marine phospholipids (MPL) are attracting increasing attention due to their health benefits and good stability. Vitamin C (Vc) and β-carotene (Bc) were concurrently encapsulated in MPL nanoliposomes ...by thin-film evaporation as a mean to overcome the limitations precluding their utilization in food. Therefore, the physicochemical characteristics, antioxidant activities and storage stability of MPL nanoliposomes were assessed against those of traditional egg phospholipids (EPC). The mean size of nanoliposomes was 154.43–253.3 nm and zeta potential from – 55.58 to – 42.74 mV. The highest entrapment efficiency recorded for Vc was 63.33% and 87.8% for Bc. MPL nanoliposomes showed higher DPPH scavenging, hydroxyl radical and lipid peroxidation inhibition capacity as well as a superior stability at 4 °C for 42 days. Overall, MPL nanoliposomes encapsulating Vc and Bc displayed better results compared to EPC. As a result, MPL nanoliposomes could be used as promising carriers for simultaneous delivery of food bioactive compounds.
•Vitamin C and β-carotene co-loaded marine and egg phospholipids nanoliposomes.•Marine phospholipids nanoliposomes display lower mean size and polydispersity index.•Marine phospholipids nanoliposomes have better antioxidant activity.•Leakage ratios of marine phospholipids nanoliposomes are lower.•Overall, marine nanoliposomes exhibit better stability at 4 °C for 42 days.
Chemical investigation on the leaves of Michelia champaca L. (Magnoliaceae) led to the isolation of five previously undescribed phenylethanoid glycosides (PhGs), 4-O-β-d-glucopyranosyl-acteoside (1), ...4‴-O-(6-O-E-caffeoyl)-β-d-glucopyranosyl-acteoside (2), 4‴-O-(6-O-E-caffeoyl)-β-d-glucopyranosyl-isoacteoside (3), 6""-O-E-feruloyl-echinacoside (4), and 6""-O-p-E-coumaroyl-echinacoside (5), together with eighteen known PhGs. Their structures were determined by spectroscopic and chemical methods. All the known PhGs except acteoside (8) were not previously reported in the genus. Twenty-one PhGs exhibited more potent DPPH radical scavenging activity and FRAP than l-ascorbic acid (l-AA), and twenty-two PhGs showed better ABTS radical cation scavenging activity than l-AA. In addition, twelve PhGs displayed more potent cellular reactive oxygen species scavenging activity than curcumin. The results revealed that the leaves of M. champaca are a rich source of phenylethanoid glycosides and antioxidants.
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•Five undescribed phenylethanoid glycosides (PhGs) were isolated from the leaves.•Seventeen known PhGs isolated were not reported from the genus Michelia.•The PhGs showed potent DPPH, ABTS, and cellular ROS scavenging activities.•The results revealed the leaves to be a rich source of PhGs and antioxidants.
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