•Resveratrol is a polyphenol from stilbenoid class.•Compound is a privileged molecule with antioxidant activity.•Resveratrol improves cardiovascular and neurodegenerative diseases.•Polyphenol has ...utility in hypertension and renal disorders.•Stilbenoid is an active osteogenic and pregnancy therapeutic agent.
Oxidative stress has a significant impact on the occurrence of many chronic diseases. Antioxidants have ability to prevent, postpone or treat effects of free radicals. Resveratrol is a polyphenol from the class of stilbenes. The importance of this compound is determined by its numerous biological properties. The strong antioxidant potential is of special significance, because of the way it acts in the prevention and treatment of numerous chronic diseases. The antioxidant action of compound is direct, by inhibiting the production of reactive species, and indirect, by influencing the activity of numerous antioxidant enzymes. In the case of analyzing antioxidant potential by known methods, it was observed that RSV had a higher capacity than the standard used. Also, in vitro and in vivo studies demonstrate the activity of compound in prevention and treatment of neurodegenerative, cardiovascular and renal diseases. In addition, RSV is active in preeclampsia and infertility. An important aspect is the fact that polyphenol has the ability to reduce and counteract the adverse effects of some anticancer agents.
Diuretic medications are widely used and can come with negative effects. Because they are effective and have fewer adverse effects than other treatments for renal illness, medicinal plants have ...become increasingly important. This study aimed to investigate the antioxidant ability and the impact of Laurus nobilis extract (flower) on diuresis in rats. Two doses of 200 mg and 400 mg of Laurus nobilis extract were used to treat rats for thirty days. Then, we assessed all changes induced in urine and plasma parameters of rats, using furosemide as a standard drug. Further, we evaluated the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant ability (DPPH and FRAP) of the tested extract. The results obtained show that the administration of a single dose of Laurus nobilis extract improved the urine flow significantly after 4 h of treatment. Similarly, both doses of the tested extract enhanced sodium, potassium, and chloride excretion without inducing hypokalemia. A similar tendency was recorded for both urine and creatinine, while the results of the furosemide group revealed a significant hypokalemia effect of the standard drug. Laurus nobilis demonstrated superior antioxidant and diuretic effects without inducing hypokalemia due to the higher content of phenolic and flavonoid content. However, more advanced studies are required to explore the constituents of Laurus nobilis extracts and essential oils, as well as to test their pertinent biological activities.
Diuretic medications are widely used and can come with negative effects. Because they are effective and have fewer adverse effects than other treatments for renal illness, medicinal plants have become increasingly important. This study aimed to investigate the antioxidant ability and the impact of Laurus nobilis extract (flower) on diuresis in rats. Two doses of 200 mg and 400 mg of Laurus nobilis extract were used to treat rats for thirty days. Then, we assessed all changes induced in urine and plasma parameters of rats, using furosemide as a standard drug. Further, we evaluated the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant ability (DPPH and FRAP) of the tested extract. The results obtained show that the administration of a single dose of Laurus nobilis extract improved the urine flow significantly after 4 h of treatment. Similarly, both doses of the tested extract enhanced sodium, potassium, and chloride excretion without inducing hypokalemia. A similar tendency was recorded for both urine and creatinine, while the results of the furosemide group revealed a significant hypokalemia effect of the standard drug. Laurus nobilis demonstrated superior antioxidant and diuretic effects without inducing hypokalemia due to the higher content of phenolic and flavonoid content. However, more advanced studies are required to explore the constituents of Laurus nobilis extracts and essential oils, as well as to test their pertinent biological activities.
Chlorella sp. microalgae is a potential source of natural antioxidants and other bioactive compounds used in the food and pharmaceutical industries. Ultrasound pretreatments followed by enzymolysis ...are widely used to improve the functional properties of biological materials. This work aimed to investigate the effects of ultrasound pretreatments followed by bromelain hydrolysis on the proteins, phenolic content, and antioxidant activity of Chlorella sp. crude extract. The experimental design for the study involved two factors: ultrasound pretreatment time (0, 5, and 10 min) and bromelain hydrolysis time (0, 2, 4, and 6 hours). The protein was measured using Lowry assay while the total phenolic content was measured using a Folin–Ciocalteu assay. The highest protein content (7.09 ± 0.44 mg/mL) and phenolic concentrations (0.28 ± 0.01 ìg/mL) were obtained at 5 min ultrasound pretreatment and 6 hours enzymolysis. For antioxidant activity, 5 min ultrasound pretreatment followed by 4 hours of enzymolysis resulted in the highest DPPH (1,1-diphenyl-2-picrylhydrazyl radical) inhibitory activity (81.74%). Meanwhile, ultrasound pretreatment for 10 min followed by 6 hours of enzymolysis resulted in the highest ABTS (2,20 azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) inhibitory activity (94.10%). This study showed that variation in ultrasound pretreatments followed by bromelain hydrolysis could be used to improve the protein content, phenolic content, and antioxidant activity of Chlorella sp. crude extract.
Foods rich in antioxidants play an essential role in the prevention of diseases. The present study compared contents of phenolics, vitamin C, anthocyanin and antioxidant activity of 11 fresh exotic ...fruits, cultivated in the northeastern part of Brazil. The antioxidant activities were evaluated using two antioxidant systems 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), expressed as TEAC (Trolox Equivalent Antioxidant Capacity) and VCEAC (Vitamin C Equivalent Antioxidant Capacity) values. The results indicated that the above fruits, such as murici and mangaba, were good sources of antioxidants. The phenolic contents showed positive correlations with total antioxidant by ABTS (R=0.94, P≤0.001) and DPPH (R=0.88, P≤0.001) assays. However, this correlation was not noticed when examining vitamin C and anthocyanins contents. The 11 fruits studied had comparable antioxidant activity in both, ABTS and DPPH assays. These methods are recommended as useful tools for the evaluation of the total activity antioxidant in fruits. Our results indicated promising perspectives for the development and usage of fruits species studied with considerable levels of antioxidant activity.
In this work, a straightforward approach utilizing distillers' grains (DG) waste and sodium alginate (SA) was developed to prepare functional and bioactive packaging films. Deep eutectic solvents ...(DESs) were initially synthesized from choline chloride (CO), betaine (BO), glycerol (GO), and oxalic acid. Composite films were then prepared from DES-treated DG slurry and SA at different ratios. Characterization and analysis revealed that adding 75 % CO-treated DG slurry reduced the water vapor permeability (WVP) by over 66 % compared to that of the SA film. Composite films containing CO/BO-treated DG slurry had an ultraviolet light barrier rate exceeding 99 %, while those with 75 % DES-treated DG slurry demonstrated excellent antioxidant activity, with a 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging rate of 80.14 %–88.35 %, representing a 322.45 %–365.73 % increase compared to that of the pure SA film. These composite films also exhibited favorable mechanical properties (31.58 MPa, 5.53 % EB), thermal stability, and biodegradability, extending the shelf life of grapes by 1.8 times. In conclusion, bioactive composite films derived from DES-treated DG are expected to replace petroleum-based plastics, enhancing sustainable biomass use and environmental responsibility.
The effects of selenium biofortification methods involving sodium selenite and selenium yeast on the structural characteristics, antioxidant activity and binding capacity of Pleurotus eryngii ...polysaccharides were investigated. Sodium selenite Se-enriched Pleurotus eryngii polysaccharides (Se-SPEP), selenium yeast Se-enriched Pleurotus eryngii polysaccharides (Se-YPEP), and Pleurotus eryngii polysaccharides (PEP) had Se contents of 20.548 ± 1.561, 19.822 ± 0.613, and 0.052 ± 0.016 μg/g, respectively. Compared with PEP, Se-SPEP and Se-YPEP had lower molecular weight and contained the same monosaccharides in varying molar ratios. The results of FT-IR, PS, ZP, and SEM indicated significant alterations in structural characteristics following selenium biofortification. Se-PEPs exhibited superior activity against ABTS, DPPH, and ·OH radicals, as well as the higher binding capacity for Cd2+ and Cu2+ compared to natural polysaccharides. The binding capacity of the polysaccharides for Cd2+ and Cu2+ was higher at pH 6.8 compared to pH 2.0, while the opposite was observed for Pb2+. Furthermore, Se-PEPs exhibited a significantly higher binding capacity for Cd2+ and Cu2+ at both pH levels compared to natural polysaccharides (P < 0.05). Se-YPEP displayed higher antioxidant activity than Se-SPEP, with their binding capacities reversed. These data indicated that selenium biofortification methods have different positive impacts on the structure and activity of polysaccharides compared to natural polysaccharides, making Se-PEPs promising dietary supplements for safeguarding the body against the risks posed by food-derived heavy metals.
•This study aims to design and synthesize triazole-based ligands via ‘click’ chemistry.•All compounds were evaluated for their anticancer, antioxidant and α-amylase activity, and most of them showed ...good inhibitory activity.•Among synthesized compounds, compound 5a was the most potent antioxidant inhibitor.•in-silico ADME and molecular docking studies were carried out.
In this study, propargyl compounds were synthesized from 4-hydroxybenzaldehyde and 3‑methoxy-4-hydroxybenzaldehyde (2a-2b). As a result of click reactions of synthesized propargyl compounds (2a-b) with organic azides (4a-4e), carbonyl compounds (5a-5 h) having 1,2,3-triazole skeleton were obtained. The structures of the synthesized compounds were illuminated by FTIR, 1H/13C NMR spectroscopies and elemental analyses. Antioxidant and anti-cancer and α-amylase enzyme inhibition studies of the synthesized compounds were carried out, and the effects of different functional groups in the compounds on the activity were investigated. When the results of the α-amylase enzyme inhibition studies of the synthesized compounds were compared with the reference drug acarbose (IC50: 891 µg/mL), it was determined that all compounds (IC50: 165–1471 µg/mL) showed higher activity than acarbose, except for compounds 5a and 5c. In particular, compound 5 g (IC50: 165 µg/mL) was found to have approximately 5.5 times higher activity than acarbose. When the DPPH• radical scavenging studies were examined, all compounds showed a higher activity than the standard BHT and β-carotene. According to ABTS•+ radical scavenging activity results, all compounds showed more effective activity than Ascorbic acid, and Trolox used as standard. Compound 5a showed approximately the same scavenging effect with β-carotene and BHT. Compounds were also screened for anti-cancer activities against the HeLa cell line. According to the results, 5c (IC50: 50.12 µg/mL) and 5 h (IC50: 57.07 µg/mL) exhibit moderate antitumor activity compared to cis-platin against the HeLa cell line. The molecules have been studied in detail for their ADME properties and have not violated any drug-likeness rules. In addition, they exhibited a high oral bioavailability profile, as their BBB (Blood Brain Barrier) penetration and GI (gastrointestinal) absorption properties were favorable. Molecular docking results show that all compounds have a high affinity for the active site of α-amylase.
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The antioxidant activity of anthocyanins in food is well known. Numerous antioxidant assays have been proposed to measure the capacity of anthocyanins to prevent the oxidation process that naturally ...occurs. Different solvents, temperatures, and pH levels are applied in each assay, and these factors should be taken into account in order to obtain useful and reproducible results. The concentration and the structure of these compounds are directly related to their antioxidant capacity and their environment. However, the effectiveness of the anthocyanin ingestion against diseases is also influenced by its bioavailability. Novel methodologies that simulate the digestion process have been developed in order to facilitate the current knowledge of anthocyanins bioavailability. Studies highlight the potential synergy effect between parent compounds and their derivatives (metabolites, conjugated products, and microbe-generated metabolites). The aim of this review is to provide an overview of advantages and disadvantages of the most common methods to determine the antioxidant activity of anthocyanins, chemical structure, and concentration of these compounds in different edible fruits, vegetables, and plants; their bioavailability after intake; as well as the main therapeutic effect described in the scientific literature.
Hydrolyzed collagen (HC) is a group of peptides with low molecular weight (3-6 KDa) that can be obtained by enzymatic action in acid or alkaline media at a specific incubation temperature. HC can be ...extracted from different sources such as bovine or porcine. These sources have presented health limitations in the last years. Recently research has shown good properties of the HC found in skin, scale, and bones from marine sources. Type and source of extraction are the main factors that affect HC properties, such as molecular weight of the peptide chain, solubility, and functional activity. HC is widely used in several industries including food, pharmaceutical, cosmetic, biomedical, and leather industries. The present review presents the different types of HC, sources of extraction, and their applications as a biomaterial.
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