A breakthrough in cosmeceuticals by utilizing insects as major ingredients in cosmetic products is gaining popularity. Therefore, the interest in rare sources of ingredients, for instance, from the ...Oryctes rhinoceros beetle, can bring huge benefits in terms of turning pests into wealth. In this study, curcumin was chosen as the active ingredient loaded into chitosan-gold nanoparticles (CCG-NP). Curcumin is unstable and has poor absorption, a high rate of metabolism, and high sensitivity to light. These are all factors that contribute to the low bioavailability of any substance to reach the target cells. Therefore, chitosan extracted from O. rhinoceros could be used as a drug carrier to overcome these limitations. In order to overcome these limitations, CCG-NPs were synthesized and characterized. Chitosan was isolated from O. rhinoceros and CCG-NPs were successfully synthesized at 70 °C for 60 min under optimal conditions of a reactant ratio of 2:0.5 (0.5 mM HAuClsub.4: 0.1% curcumin). Characterizations of CCG-NP involved FTIR analysis, zeta potential, morphological properties determination by FE-SEM, particle size analysis, crystallinity study by XRD, and elemental analysis by EDX. The shape of the CCG-NP was round, its size was 128.27 d.nm, and the value of the zeta potential was 20.2 ± 3.81 mV. The IC50 value for cell viability is 58%, indicating a mild toxicity trait. To conclude, CCG-NP is a stable, spherical, nano-sized, non-toxic, and homogeneous solution.
Chitin is a major component of the exoskeleton and the peritrophic matrix of insects. It forms complex structures in association with different assortments of cuticle and peritrophic matrix proteins ...to yield biocomposites with a wide range of physicochemical and mechanical properties. The growth and development of insects are intimately coupled with the biosynthesis, turnover, and modification of chitin. The genes encoding numerous enzymes of chitin metabolism and proteins that associate with and organize chitin have been uncovered by bioinformatics analyses. Many of these proteins are encoded by sets of large gene families. There is specialization among members within each family, which function in particular tissues or developmental stages. Chitin-containing matrices are dynamically modified at every developmental stage and are under developmental and/or physiological control. A thorough understanding of the diverse processes associated with the assembly and turnover of these chitinous matrices offers many strategies to achieve selective pest control.
Petrochemical resources are non-renewable, which has impeded the development of synthetic polymers. The poor degradability of synthetic polymers poses substantial environmental pressure. ...Additionally, the high cost of synthetic biopolymers with excellent degradation performance limits their widespread application. Thus, it is crucial to seek green, sustainable, low-cost polymers as alternatives to petrochemical-based synthetic polymers and synthetic biopolymers. Chitin is a natural and renewable biopolymer discovered in crustacean shells, insect exoskeletons, and fungal cell walls. Chitin chains consist of crystalline and amorphous regions. Note that various treatments can be employed to remove the amorphous region, enhancing the crystallinity of chitin. Chitin nanowhiskers are a high crystallinity nanoscale chitin product with a high aspect ratio, a large surface area, adjustable surface morphology, and biocompatibility. They discover widespread applications in biomedicine, environmental treatment, food packaging, and biomaterials. Various methods can be utilized for preparing chitin nanowhiskers, including chemical, ionic liquids, deacetylation, and mechanical methods. However, developing an environmentally friendly preparation process remains a big challenge for expanding their applications in different materials and large-scale production. This article comprehensively analyzes chitin nanowhiskers' preparation strategies and their drawbacks. It also highlights the extensive application in different materials and various fields, besides the potential for commercial application.
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Abstract Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility. Chitin derivatives with acrylic groups, such as ...2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator. The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs). HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive. Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF. These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.
The fungal cell wall is essential for growth and survival, and is a key target for antifungal drugs and the immune system. The cell wall must be robust but flexible, protective and shielding yet ...porous to nutrients and membrane vesicles and receptive to exogenous signals. Most fungi have a common inner wall skeleton of chitin and β-glucans that functions as a flexible viscoelastic frame to which a more diverse set of outer cell wall polymers and glycosylated proteins are attached. Whereas the inner wall largely determines shape and strength, the outer wall confers properties of hydrophobicity, adhesiveness, and chemical and immunological heterogeneity. The spatial organization and dynamic regulation of the wall in response to prevailing growth conditions enable fungi to thrive within changing, diverse and often hostile environments. Understanding this architecture provides opportunities to develop diagnostics and drugs to combat life-threatening fungal infections.
Fungal pathogens are major destructive microorganisms for land plants and pose growing challenges to global crop production. Chitin is a vital building block for fungal cell walls and also a broadly ...effective elicitor of plant immunity. Here we review the rapid progress in understanding chitin perception and signaling in plants and highlight similarities and differences of these processes between arabidopsis and rice. We also outline moonlight functions of CERK1, an indispensable chitin coreceptor conserved across the plant kingdom, which imply potential crosstalk between chitin signaling and symbiotic or biotic/abiotic stress signaling in plants via CERK1. Moreover, we summarize current knowledge about fungal counterstrategies for subverting chitin-triggered plant immunity and propose open questions and future directions in this field.
Chitin perception systems in both arabidopsis and rice consist of multiple LysM-containing proteins, which undergo chitin-induced oligomerization to trigger intracellular signaling. An oligomer size-dependent chitin sensing mechanism is conserved across plants and mammals.Receptor-like cytoplasmic kinase VII members are essential players of chitin signaling in both arabidopsis and rice.Both arabidopsis and rice CERK1 proteins play multifaceted functions beyond chitin signaling and may mediate the crosstalk between chitin signaling and symbiotic or other biotic or abiotic stress signaling.Most uncovered fungal countermeasures to subvert chitin-triggered plant immunity involve blocking the generation and perception of chitin oligomers in plant apoplast, whereas fungal cytoplasmic effector-mediated strategies to disrupt chitin signaling inside plant cells remain poorly understood.
Chitin displays a highly rigid structure due to the vast intra‐ and intermolecular hydrogen bonding, thus hindering its dissolution and deacetylation using most solvents. Deep eutectic solvents ...(DESs) are special and environmentally friendly solvents composed of a hydrogen bond acceptor and a hydrogen bond donor. This allows them to dissolve chitin by disturbing its natural hydrogen bonding while establishing new bonds, hence turning the polymer more susceptible to solvents. Therefore, four distinct DESs (choline chloride‐lactic acid (ChCl:LA), choline chloride:oxalic acid (ChCl:OA), choline chloride:urea (ChCl:U) and betaine‐glycerol (Bet:G)) were applied in chitin dissolution, being the most performant ones further applied in its homogenous N‐deacetylation with NaOH. In this work, a milder and more biocompatible approach was carried out by using 30 wt% NaOH at 80°C, instead of the typical ≥40 wt% NaOH at temperatures ≥100°C. Herein, the reaction process took up to 18 hours, being the results analyzed through ATR‐FTIR. Chitin was converted into chitosan with a 70‐80% degree of deacetylation (DDA) in a short period while using homogenous conditions. These promising results provide the first proof of concept of the ability of Bet:G and ChCl:LA‐based DESs to be used as a greener approach for the chitin homogeneous N‐deacetylation.
This review describes the most common methods for recovery of chitin from marine organisms. In depth, both enzymatic and chemical treatments for the step of deproteinization are compared, as well as ...different conditions for demineralization. The conditions of chitosan preparation are also discussed, since they significantly impact the synthesis of chitosan with varying degree of acetylation (DA) and molecular weight (MW). In addition, the main characterization techniques applied for chitin and chitosan are recalled, pointing out the role of their solubility in relation with the chemical structure (mainly the acetyl group distribution along the backbone). Biological activities are also presented, such as: antibacterial, antifungal, antitumor and antioxidant. Interestingly, the relationship between chemical structure and biological activity is demonstrated for chitosan molecules with different DA and MW and homogeneous distribution of acetyl groups for the first time. In the end, several selected pharmaceutical and biomedical applications are presented, in which chitin and chitosan are recognized as new biomaterials taking advantage of their biocompatibility and biodegradability.
The peritrophic matrix (PM) is a chitin and glycoprotein layer that lines the invertebrate midgut. Although structurally different, it is functionally similar to the mucous secretions of the ...vertebrate digestive tract. The PM is a physical barrier, protecting the midgut epithelium from abrasive food particles, digestive enzymes, and pathogens infectious per os. It is also a biochemical barrier, sequestering and, in some cases, inactivating ingested toxins. Finally, the PM compartmentalizes digestive processes, allowing for efficient nutrient acquisition and reuse of hydrolytic enzymes. The PM consists of an organized lattice of chitin fibrils held together by chitin binding proteins. Glycans fill the interstitial spaces, creating a molecular sieve, the properties of which are dependent on the immediate ion content and pH. In this review, we have integrated recent structural and functional information to create a holistic model for the PM. We also show how this information may generate novel technologies for use in insect pest management.
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