As trophic organisms, nematodes play an essential role in the soil environment: they mineralize nutrients into plant-available forms, are a food source for other soil organisms, and feed on ...pathogenic organisms and plant pests, therefore regulating populations of soil microorganisms. The plant-parasitic nematodes are important pests of crops. Nanoparticles (NP) are increasingly used in agriculture and other production sectors. They are present in the soil, not necessarily in trace amounts, and can affect various soil organisms, including nematodes. In this article, the effects of silver (Ag), gold (Au), and platinum (Pt) nanoparticles on the mortality and reproduction activity of selected nematode species from two trophic groups: entomopathogenic nematodes (EPN) (
and
) and plant parasitic nematodes (PPN) (
,
,
) were studied under laboratory conditions. All nanoparticles decreased the nematode population to an extent depending on the nematode species, nanoparticle type, and exposure time. AgNP had the greatest nematicidal effect, except for AuNP, which reduced the population of
the most. The greatest sensitivity to AgNP was observed in
(100% mortality),
(90% mortality), and 37 to 13% mortality in other species. Effect of AuNP and PtNP on entomopathogenic nematodes was comparable to the control, not treated with nanoparticles. AuNP and PtNP similarly influenced nematode mortality. However, the effect of nanoparticles on new generations of entomopathogenic nematodes developing in host larvae
was inconclusive. All nanoparticles decreased the number of larvae of
and increased the number of
migrating outside the cadavers compared to the control.
Tobacco thrips, Frankliniella fusca (Hinds) is an economically significant pest. Entomopathogenic nematodes (EPNs) have shown promise as biocontrol agents against certain thrips species, but they ...have not been explored for suppression of F. fusca. We investigated the potential of EPNs to manage F. fusca by conducting three different bioassays: 1) a small cup dose–response bioassay (25, 50, and 100 IJs cm–2) with four EPN species, 2) a broad virulence bioassay with eight EPN species at 100 IJs cm–2, and 3) a potted soil bioassay testing with four EPN species (100 IJs cm–2). In the dose–response bioassay, all treatments showed relatively lower adult emergence when compared with the control group, but the minimum adult emergence (30%) was observed at 7 d post-treatment when Heterorhabditis bacteriophora (FL1-1) was applied at the highest rate (100 IJs cm–2). In the broad virulence study, all EPN treatments caused significant reductions in F. fusca adult emergence (18.3–75.0%) in comparison with the control. H. bacteriophora (Fl1-1) was more virulent than other nematode treatments but statistically not different from Steinernema feltiae and Steinernema riobrave, while Steinernema rarum was the least virulent. In the potted soil bioassay, the lowest emergence (10.6%) was observed in H. bacteriophora (Fl1-1) treatment, followed by S. feltiae (SN), S. riobrave (355), and Heterorhabditis indica (HOM1) treatments. These results indicate that EPNs have the ability to suppress the soil dwelling stage of F. fusca and should be explored further under greenhouse and field conditions for biocontrol potential within an integrated pest management (IPM) context.
Estimated world biopesticide sales by type in 2010 (millions of $US). CPL Business Consultants (2010). The 2010 Worldwide Biopesticides Market Summary, vol. 1. CAB International Centre, Wallingford. ...Display omitted
The development and use of entomopathogens as classical, conservation and augmentative biological control agents have included a number of successes and some setbacks in the past 15years. In this forum paper we present current information on development, use and future directions of insect-specific viruses, bacteria, fungi and nematodes as components of integrated pest management strategies for control of arthropod pests of crops, forests, urban habitats, and insects of medical and veterinary importance.
Insect pathogenic viruses are a fruitful source of microbial control agents (MCAs), particularly for the control of lepidopteran pests. Most research is focused on the baculoviruses, important pathogens of some globally important pests for which control has become difficult due to either pesticide resistance or pressure to reduce pesticide residues. Baculoviruses are accepted as safe, readily mass produced, highly pathogenic and easily formulated and applied control agents. New baculovirus products are appearing in many countries and gaining an increased market share. However, the absence of a practical in vitro mass production system, generally higher production costs, limited post application persistence, slow rate of kill and high host specificity currently contribute to restricted use in pest control. Overcoming these limitations are key research areas for which progress could open up use of insect viruses to much larger markets.
A small number of entomopathogenic bacteria have been commercially developed for control of insect pests. These include several Bacillus thuringiensis sub-species, Lysinibacillus (Bacillus) sphaericus, Paenibacillus spp. and Serratia entomophila. B. thuringiensis sub-species kurstaki is the most widely used for control of pest insects of crops and forests, and B. thuringiensis sub-species israelensis and L. sphaericus are the primary pathogens used for control of medically important pests including dipteran vectors. These pathogens combine the advantages of chemical pesticides and MCAs: they are fast acting, easy to produce at a relatively low cost, easy to formulate, have a long shelf life and allow delivery using conventional application equipment and systemics (i.e. in transgenic plants). Unlike broad spectrum chemical pesticides, B. thuringiensis toxins are selective and negative environmental impact is very limited. Of the several commercially produced MCAs, B. thuringiensis (Bt) has more than 50% of market share. Extensive research, particularly on the molecular mode of action of Bt toxins, has been conducted over the past two decades. The Bt genes used in insect-resistant transgenic crops belong to the Cry and vegetative insecticidal protein families of toxins. Bt has been highly efficacious in pest management of corn and cotton, drastically reducing the amount of broad spectrum chemical insecticides used while being safe for consumers and non-target organisms. Despite successes, the adoption of Bt crops has not been without controversy. Although there is a lack of scientific evidence regarding their detrimental effects, this controversy has created the widespread perception in some quarters that Bt crops are dangerous for the environment. In addition to discovery of more efficacious isolates and toxins, an increase in the use of Bt products and transgenes will rely on innovations in formulation, better delivery systems and ultimately, wider public acceptance of transgenic plants expressing insect-specific Bt toxins.
Fungi are ubiquitous natural entomopathogens that often cause epizootics in host insects and possess many desirable traits that favor their development as MCAs. Presently, commercialized microbial pesticides based on entomopathogenic fungi largely occupy niche markets. A variety of molecular tools and technologies have recently allowed reclassification of numerous species based on phylogeny, as well as matching anamorphs (asexual forms) and teleomorphs (sexual forms) of several entomopathogenic taxa in the Phylum Ascomycota. Although these fungi have been traditionally regarded exclusively as pathogens of arthropods, recent studies have demonstrated that they occupy a great diversity of ecological niches. Entomopathogenic fungi are now known to be plant endophytes, plant disease antagonists, rhizosphere colonizers, and plant growth promoters. These newly understood attributes provide possibilities to use fungi in multiple roles. In addition to arthropod pest control, some fungal species could simultaneously suppress plant pathogens and plant parasitic nematodes as well as promote plant growth. A greater understanding of fungal ecology is needed to define their roles in nature and evaluate their limitations in biological control. More efficient mass production, formulation and delivery systems must be devised to supply an ever increasing market. More testing under field conditions is required to identify effects of biotic and abiotic factors on efficacy and persistence. Lastly, greater attention must be paid to their use within integrated pest management programs; in particular, strategies that incorporate fungi in combination with arthropod predators and parasitoids need to be defined to ensure compatibility and maximize efficacy.
Entomopathogenic nematodes (EPNs) in the genera Steinernema and Heterorhabditis are potent MCAs. Substantial progress in research and application of EPNs has been made in the past decade. The number of target pests shown to be susceptible to EPNs has continued to increase. Advancements in this regard primarily have been made in soil habitats where EPNs are shielded from environmental extremes, but progress has also been made in use of nematodes in above-ground habitats owing to the development of improved protective formulations. Progress has also resulted from advancements in nematode production technology using both in vivo and in vitro systems; novel application methods such as distribution of infected host cadavers; and nematode strain improvement via enhancement and stabilization of beneficial traits. Innovative research has also yielded insights into the fundamentals of EPN biology including major advances in genomics, nematode-bacterial symbiont interactions, ecological relationships, and foraging behavior. Additional research is needed to leverage these basic findings toward direct improvements in microbial control.
The research objects were entomopathogenic organisms (Nematoda, Fungi, Bacteria, and Insecta) collected in agrocoenoses in different regions of Ukraine during 2016–2018 and 2020–2021. The following ...research materials were used: soil samples, soil live-traps, specimens of Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) and potential host insects (Coleoptera: Elateridae, Tenebrionidae, Melolonthinae; Lepidoptera: Noctuidae). We analysed 312 samples (220 soil + 92 live-trap samples) and >100 specimens of potential host insects. Our data demonstrate that in soils of agrocoenoses, favourable conditions are created for the dispersal of entomopathogenic organisms. We report the frequency of occurrence of insect-pathogenic nematodes in agrocoenoses of Ukraine, and describe their identification and the specifics of pathology they cause to the insect Galleria mellonella in the context of other entomopathogenic organisms. The frequency of occurrence (% of samples) of entomopathogenic nematodes (genus Steinernema Travassos, 1927 and genus Heterorhabditis Poinar, 1976) in agrocoenoses was the highest among other organisms that caused infectious and parasitic diseases of insects and were found in 15% of the samples. Three species of entomopathogenic nematodes—Steinernema carpocapsae (Weiser, 1955) Wouts et al., 1982, Steinernema ex gr. ‘glaseri‘, and Heterorhabditis bacteriophora Poinar, 1976—have been identified. We have found 8% of samples to contain fungal disease-causing agents (genera Beauveria Vuill., 1912, Metarhizium Sorokin, 1879, and Akanthomyces Lebert, 1858). The frequency of occurrence (%) of all remaining causative agents of infectious and parasitic diseases of insects, namely bacterial diseases and myiasis (infection of a fly larva) (Diptera: Tachinidae) were 3% and 2%, respectively. A mixed infection was detected in 2.5% of the total number of analysed samples; nematodoses-mycoses mixed infections were most often recorded. We have recorded the phenomenon of hyperparasitism with nematodosis-entomosis co-infection inside dead G. mellonella larvae for the first time.
One of the most important development trends of robots in agriculture is to enable highly precise applications that minimize amounts of chemical components that are harmful to the environment. ...Precision agriculture is fundamental and inevitable worldwide because it provides more yield to an increasing population, while at the same time reducing inputs. The purpose of this study was to apply entomopathogenic nematodes, which are insect parasites used as biological control agents, through Nemabot. A robotic system that can move in the X-Y-Z coordinate plane has an agitating mechanism for suspension based on water and entomopathogenic nematodes and can perform precise dosing with a peristaltic pump designed and produced as a prototype. The experimental results of the robot application on the exact point, volume, amount, and uniformity of dosing show that the proposed method can effectively solve the problem of applying entomopathogenic nematodes, which are economically more expensive than pesticides. The main contribution of this paper is the proposal of a method to solve the problem of applying the agents precisely. This is the first experiment in which biological control products were applied using a robotic system. A patent application (PCT/TR2019/050768) was made, and the patentability claims were approved and officially registered (TR2018 14310B).
•Emphasizing the importance of robotics in agriculture for precise, eco-friendly applications.•Presenting ‘Nemabot,’ a robot for precise entomopathogenic nematode application in biocontrol.•Suggesting efficient use of cost-effective EPNs as a sustainable alternative to pesticides in agriculture.•The article marks a significant advancement in using robotic systems for biological control products.
Microorganisms contribute to the biology and physiology of eukaryotic hosts and affect other organisms through natural products. Xenorhabdus and Photorhabdus (XP) living in mutualistic symbiosis with ...entomopathogenic nematodes generate natural products to mediate bacteria-nematode-insect interactions. However, a lack of systematic analysis of the XP biosynthetic gene clusters (BGCs) has limited the understanding of how natural products affect interactions between the organisms. Here we combine pangenome and sequence similarity networks to analyse BGCs from 45 XP strains that cover all sequenced strains in our collection and represent almost all XP taxonomy. The identified 1,000 BGCs belong to 176 families. The most conserved families are denoted by 11 BGC classes. We homologously (over)express the ubiquitous and unique BGCs and identify compounds featuring unusual architectures. The bioactivity evaluation demonstrates that the prevalent compounds are eukaryotic proteasome inhibitors, virulence factors against insects, metallophores and insect immunosuppressants. These findings explain the functional basis of bacterial natural products in this tripartite relationship.
Microbial biopesticides include several microorganisms like bacteria, fungi, baculoviruses, and nematode-associated bacteria acting against invertebrate pests in agro-ecosystems. The biopesticide ...sector is experiencing a significant growth and many discoveries are being developed into new biopesticidal products that are fueling a growing global market offer. Following a few decades of successful use of the entomopathogenic bacterium Bacillus thuringiensis and a few other microbial species, recent academic and industrial efforts have led to the discovery of new microbial species and strains, and of their specific toxins and virulence factors. Many of these have, therefore, been developed into commercial products. Bacterial entomopathogens include several Bacillaceae, Serratia, Pseudomonas, Yersinia, Burkholderia, Chromobacterium, Streptomyces, and Saccharopolyspora species, while fungi comprise different strains of Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Verticillium, Lecanicillium, Hirsutella, Paecilomyces, and Isaria species. Baculoviruses are species-specific and refer to niche products active against chewing insects, especially Lepidopteran caterpillars. Entomopathogenic nematodes (EPNs) mainly include species in the genera Heterorhabditis and Steinernema associated with mutualistic symbiotic bacteria belonging to the genera Photorhabdus and Xenorhabdus. An updated representation of the current knowledge on microbial biopesticides and of the availability of active substances that can be used in integrated pest management programs in agro-ecosystems is reported here.
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•Metarhizium VOCs, 1-octen-3-ol and 3 octanone, influence behaviour of EPN.•High doses of 1-octen-3-ol and 3 octanone repel or kill EPN infective juveniles.•Metarhizium volatiles ...reduce EPN infectivity of insect hosts.•Metarhizium VOCs immobilise or kill insects.•Volatiles affect reproduction of EPN inside host.
The entomopathogenic fungus (EPF) Metarhizium brunneum occupies the same ecological niche as entomopathogenic nematodes (EPN), with both competing for insects as a food source in the rhizosphere. Interactions between these biocontrol agents can be antagonistic or synergistic. To better understand these interactions, this study focussed on investigating the effect of M. brunneum volatile organic compounds (VOCs), 1-octen-3-ol and 3-octanone, on EPN survival and behaviour. These VOCs proved to be highly toxic to the infective juveniles (IJs) of the EPN Steinernema carpocapsae, Steinernema feltiae and Heterorhabditis bacteriophora with mortality being dose dependent. Chemotaxis studies of H. bacteriophora IJs in Pluronic F127 gel revealed significant preference for the VOCs compared with controls for all tested concentrations. The VOCs also impacted on the test insects in a dose-dependent manner with 3-octanone being more toxic to Galleria mellonella, Cydia splendana and Curculio elephas larvae than 1-octen-3-ol. Mortality of C. splendana and G. mellonella larvae was significantly higher when exposed to relatively high doses (>25%) of 3-octanone. Lower doses of 3-octanone and 1-octen-3-ol immobilised test insects, which recovered after exposure to fresh air for 2 hrs. In depth studies on H. bacteriophora showed that exposure of IJs to > 10% concentration of 3-octanone or 1-octen-3-ol negatively affected infectivity whereas exposure to lower doses (0.1%, 0.01%) had no effect. The VOCs affected IJs, reducing penetration efficacy and the number of generations inside G. mellonella but they failed to inhibit the bacterial symbiont, Photorhabdus kayaii. The ecological significance of VOCs and how they could influence EPF-EPN insect interactions is discussed.
Two families of entomopathogenic nematodes (EPNs), Steinernematidae and Heterorhabditidae, that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria, are one of the effective ...biological control agents of insect pests. Native isolates can probably be more efficacious to control insect pests than exotic ones due to their adaptability to indigenous environmental conditions. In this study, Steinernema feltiae isolate FUM221, was recovered from soil samples collected from the Ardabil Province, Iran. Morphological and morphometric investigations of the first and second-generation adults, infective juveniles, and molecular characterizations were given based on ITS and 18S rDNA genes. Besides, molecular analysis based on the 16S rRNA region and phenetic data recognized Xenorhabdus bovienii as its symbiont bacterium. The scanning electron microscopy (SEM) images verified the identification of this isolate. The molecular characterization using two loci and phylogenetic analyses provided more evidence for the classification of this steinernematid and its difference of the same species from other countries. Moreover, molecular and phenetic characterizations of its symbiotic bacterium were provided with low variations compared to other isolates. Herein, the comprehensive taxonomic data of this steinernematid and its symbiont bacterium, is presented from Iran.
Aim of study: The green vegetable bug Nezara viridula L. is a polyphage that is spread all over the world, but in the last 10 years it has entered the territory of the Russian Federation. The use of ...biological protection against this pest is an important task in the country. We used the entomopathogenic nematodes (EPN) Steinernema feltiae and Steinernema carpocapsae to control N. viridula. Area of study: Federal Research Center of Biological Plant Protection (FRCBPP), Krasnodar Krai, Russia, 2019-2020. Material and methods: A laboratory test was carried out with adults and nymphs of N. viridula. Each species of EPN (S. carpocapsae and S. feltiae) was used at doses of 50, 75 and 100 individuals infective larvae per insect. The initial material for analysis was collected on soybean crops in the crop rotation of the FRCBPP. The experimental results were assessed using ANOVA. Main results: Laboratory tests of the EPN S. carpocapsae and S. feltiae caused the death of up to 98.0% of nymphs and up to 91.4% of adults of N. viridula. The species S. feltiae turned out to be the most effective, as allowed the pathogen to develop in shorter periods of time, and caused the death of 81.9-91.4% adults and of 92.0-98.0% nymphs. Research highlights: This study showed that during the period of growth and development of larvae, the use of EPN is more effective in nymphs than on adults.
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