In order to study the effects of GABA-rich germinated adzuki bean on blood glucose level and intestinal flora in T2DM mice. C57BL/6J mice were used as subjects to construct T2DM model by high fat ...diet+STZ injection. Different doses of GABA-rich germinated adzuki bean were used to treat T2DM mice for 6 weeks and 16S rRNA sequencing technique was used to identify the microbial structure and distribution in the cecal contents of T2DM mice. The results showed that different doses of GABA-rich adzuki bean dietary intervention could significantly reduce the FBG value of T2DM mice, and the FBG value of high dose GABA-rich adzuki bean (TF3) group was 8.36±0.78 mmol/L, which was 54.09%, lower than that of model group (M). In addition, TF3 diet induced significant changes in intestinal flora abundance in T2DM mice. Firmicutes abundance at gate level was 35.96%, which decreased by 53.17% compared with M model group, and Bacteroidetes and Verrucomicrobia abundance were significantly up-regulated. In summary, the improvem
Chromium (Cr) toxicity impairs the productivity of crops and is a major threat to food security worldwide. However, the effect of Cr toxicity on seed germination and transcriptome of germinating ...seedlings of soybean crop has not been fully explored. In this study, two Cr-tolerant lines (J82, S125) and two Cr-sensitive ones (LD1, RL) were screened out of twenty-one soybean (Glycine max L.) genotypes based on seed germination rate, seed germinative energy, seed germination index, and growth of germinating seedlings under 50 mg L−1 Cr treatment. We found that Cr stress inhibits the growth of soybean seed germinating seedlings due to the Cr-induced overaccumulation of reactive oxygen species (ROS). Significantly different levels of element contents, antioxidant enzyme activities, malondialdehyde content were observed in the four soybean genotypes with contrasting Cr tolerance. Further, a total of 13,777 differentially expressed genes (DEGs) were identified in transcriptomic sequencing and 1298 DEGs in six gene modules were found highly correlated with the physiological traits by weighted correlation network analysis (WGCNA) analysis. The DEGs encoding antioxidant enzymes, transcription factors, and ion transporters are proposed to confer Cr tolerance in soybean germinating seedlings by reducing the uptake and translocation of Cr, decreasing the level of ROS, and keeping the osmotic balance in soybean germinating seedings. In conclusion, our study provided a molecular regulation network on soybean Cr tolerance at seed germinating stage and identified candidate genes for molecular breeding of low Cr accumulation soybean cultivars.
Apart from the physiological functions of soybean phytoalexins, the production sites in soybeans remain unknown. In this study, the dynamic production of phytoalexins, glyceollins, in germinating ...soybeans inoculated with Aspergillus oryzae was visually investigated using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging. During a 3-day sensitization using a fungus, glyceollins I–III were produced in germinating soybeans (from 0.03 mg/g for glyceollin III to 0.96 mg/g for glyceollin I). Imaging analysis provided visual evidence that glyceollins were produced only in the regions of seed coat and germinated root of the soybeans, while no production was observed in other regions, including the cotyledons. In contrast, their precursor, isoflavone, was distributed throughout the soybean. The evidence that the inoculation of the inactivated fungi also caused glyceollin production at the seed coat led us to speculate that glyceollins could be produced in the region of soybean attached to the fungus body.
•The influence rules of UV-B radiation dose on the isoflavone monomers were evaluated.•LF-NMR was used to characterize the soybean growth situation and cell damage level.•Combined isoflavone ...accumulation with oxidative-antioxidant system under UV-B.
This study investigated the relationships among UV-B radiation dose, isoflavone monomers and the oxidative-antioxidant system in germinating soybean. Results showed that the isoflavone monomers content showed a good fit to the quadratic model with UV-B radiation dose, except for aglycones. UV-B decreased phenylalanine content and up-regulated the key enzymes activities in isoflavone biosynthesis. H2O2, electrolyte leakage, malondialdehyde, T22 and M22 were increased, while T23 and M23 decreased. Microscopic analysis showed excess UV-B radiation resulted in the reduced cell volume, irregular cell shape, and increased cell space. The antioxidant enzymes activities were enhanced by UV-B. These results demonstrated that UV-B could trigger the formation of H2O2, resulting in the oxidative stress. Thus, the antioxidant system, including the enzymatic (enhanced the antioxidant enzymes activities) and nonenzymatic (accumulated isoflavones) were activated to minimize oxidative damage. This study provides theoretical basis for enhancing isoflavone monomer accumulation in plant-source foods by UV-B.
Ridwan, Witjaksono. 2020. Induction of autotetraploid Moringa plant (Moringa oleifera) using oryzalin. Biodiversitas 21: 4086-4093. Moringa (Moringa oleifera Lam.) is a plant with high nutritional ...content and has been widely used as a vegetable and health food ingredient, but its utilization and productivity have not been optimal. Increasing plant productivity can be done by increasing biomass which can be obtained through increasing plant ploidy. This study aims at obtaining tetraploid Moringa by induction using oryzalin compound. Moringa germinating seeds were soaked in oryzalin water solutions for 1, 3, and 5 days with concentrations of 0 µM (control), 15 µM, 30 µM, 60 µM, and 120 µM with 10 seeds for each treatment. We have recovered two tetraploid Moringa plants and two chimeric plants of diploid and tetraploid genomes. Immersing germinating seeds for 1 day in 15 or 60 µM oryzalin concentration effectively induced autopolyploid in Moringa. Tetraploid plants exhibit typical morphological characteristics of a tetraploid, such as low stomata density, larger stomata size, and larger leaflet size compared to the diploid counterpart. The induced tetraploid plants have significantly increased protein, fat, and calcium content compared to the diploid counterpart emphasizing their values as functional food. This polyploidy induction method can be used to produce Moringa tetraploid lines from various accessions in the attempts of developing and selecting high yielding tetraploid Moringa cultivars.
A strawberry RIPK1, a leu-rich repeat receptor-like protein kinase, is previously demonstrated to be involved in fruit ripening as a positive regulator; however, its role in vegetable growth remains ...unknown. Here, based on our first establishment of
Agrobacterium
-mediated transformation of germinating seeds in diploid strawberry by
FvCHLH
/
FvABAR
, a reporter gene that functioned in chlorophyll biosynthesis, we got
FvRIPK1-RNAi
mutants. Downregulation of
FvRIPK1
inhibited plant morphogenesis, showing curled leaves; also, this silencing significantly reduced
FvABAR
and
FvABI1
transcripts and promoted
FvABI4
,
FvSnRK2.2
, and
FvSnRK2.6
transcripts. Interestingly, the downregulation of the
FvCHLH/ABAR
expression could not affect
FvRIPK1
transcripts but remarkably reduced
FvABI1
transcripts and promoted
FvABI4
,
FvSnRK2.2
, and
FvSnRK2.6
transcripts in the contrast of the non-transgenic plants to the
FvCHLH
/
FvABAR
-RNAi plants, in which chlorophyll contents were not affected but had abscisic acid (ABA) response in stomata movement and drought stress. The distinct expression level of
FvABI1
and
FvABI4
, together with the similar expression level of
FvSnRK2.2
and
FvSnRK2.6
in the
FvRIPK1
- and
FvABAR/CHLH
-RNAi plants, suggested that FvRIPK1 regulated plant morphogenesis probably by ABA signaling. In addition, FvRIPK1 interacted with FvSnRK2.6 and phosphorylated each other, thus forming the FvRIPK1–FvSnRK2.6 complex. In conclusion, our results provide new insights into the molecular mechanism of FvRIPK1 in plant growth.
Summary
Plants are considered an important food and nutrition source for humans. Despite advances in plant seed metabolomics, knowledge about the genetic and molecular bases of rice seed metabolomes ...at different developmental stages is still limited. Here, using Zhenshan 97 (ZS97) and Minghui 63 (MH63), we performed a widely targeted metabolic profiling in seeds during grain filling, mature seeds and germinating seeds. The diversity between MH63 and ZS97 was characterized in terms of the content of metabolites and the metabolic shifting across developmental stages. Taking advantage of the ultra‐high‐density genetic map of a population of 210 recombinant inbred lines (RILs) derived from a cross between ZS97 and MH63, we identified 4681 putative metabolic quantitative trait loci (mQTLs) in seeds across the three stages. Further analysis of the mQTLs for the codetected metabolites across the three stages revealed that the genetic regulation of metabolite accumulation was closely related to developmental stage. Using in silico analyses, we characterized 35 candidate genes responsible for 30 structurally identified or annotated compounds, among which LOC_Os07g04970 and LOC_Os06g03990 were identified to be responsible for feruloylserotonin and l‐asparagine content variation across populations, respectively. Metabolite−agronomic trait association and colocation between mQTLs and phenotypic quantitative trait loci (pQTLs) revealed the complexity of the metabolite−agronomic trait relationship and the corresponding genetic basis.
Significance Statement
Despite advances in plant metabolomics, knowledge of the diversity of plant metabolites in the same tissue at different developmental stages and of its genetic basis are limited. Here, we revealed the genetic regulation of the metabolic diversity in rice seeds across different biological processes and found that the genetic regulation of metabolite accumulation was closely related to developmental stage, which has significant importance for crop improvements.
•Spermidine could improve antioxidant capacity of mung bean sprouts.•Spermidine reduced antioxidant enzyme activities and gene expressions.•The energy metabolism was enhanced by ...spermidine.•Dicyclohexylamine application blocked the function of spermidine.
The effects of exogenous spermidine and dicyclohexylamine (DCHA, spermidine synthesis inhibitor) on the antioxidative system and energy status of germinating mung bean were investigated. Results showed that exogenous spermidine increased the content of total phenolic and ascorbic acid and the antioxidative activity, but reduced activities and gene expressions of peroxidase (POD) and catalase (CAT). These changes might be explained by increased H2O2 content and activities of succinic dehydrogenase (SDH), adenosine triphosphatases (ATPases), and cytochrome c oxidase (CCO), resulting in higher adenosine triphosphate (ATP) and energy charge (EC). Interestingly, spermidine down-regulated expressions of SDH, H+-ATPase, Ca2+-ATPase and CCO whilst DCHA reduced energy metabolism and induced the opposite effects to spermidine, except for ascorbic acid content. Inhibition was reversed by exogenous spermidine. In conclusion, spermidine induced the accumulation of H2O2, enhanced the antioxidative system and improved the energy metabolism to enhance the functional quality of mung bean sprouts.
Multi-generational asexual reproduction of Gastrodia elata Bl. will cause seedling species degeneration. Sexual reproduction of Gastrodia elata Bl. seed is an effective method to solve the problem of ...degeneration. The development of Gastrodia elata Bl. seeds cannot be separated from the germination fungus. However, there are few strains of germination fungus in production, and there is also the problem of species degradation in application for many years. It is very important for the sexual reproduction of Gastrodia elata Bl. to isolate more new strains of excellent germination fungus from the origin. This study used the Gastrodia elata Bl. f. glauca S. chow seeds germination vegetative propagation corms capture method to isolate its symbiotic germination fungus, and comprehensively identified the species of germination fungus by colony morphology, ITS, sporocarps regeneration and germination function, and compared the growth characteristics and germination ability with other germination fungus (Mycena purpureofusca, Mycena dendrobii and Mycena osmundicola). The germination fungus was isolated from the vegetative propagation corms of Gastrodia elata Bl. f. glauca S. chow seeds and named GYGL-1. After comprehensive identification, GYGL-1 was Mycetinis scorodonius. Compared with other germination fungus, GYGL-1 has fast germination speed, vigorous growth, and high germination ability for Gastrodia elata Bl. f. glauca S. chow seeds. Innovated the isolation method of Gastrodia elata Bl. seeds germination fungus, obtained the regenerated sporocarps of the germination fungus, and discovered that Mycetinis scorodonius has a new function of germinating Gastrodia elata Bl. f. glauca S. chow seeds, enriching the resource library of Gastrodia elata Bl. germination fungus.
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