Almost all kinds of microorganisms from blood can be isolated by hemoculture. The isolate consists of 75–85% Gram negative and Gram positive bacteria, until the other part of the isolate consists of ...fungi or other microorganisms.During the three-year period (1998–2001) we analyzed microbiological characteristics of all positive hemocultures inspected in microbiological laboratory in the Public Health Institute in Nis as well as clinical parameters of patients with the aim of determining the cause of bacteremia. We applied standard microbiologic methods for inspection of 1995 hemocultures taken from 759 patients whereupon we registered positive medical findings in 18,75%. In the majority of cases only one kind of microorganisms was isolated, while in 1.06% of positive hemocultures we isoleted two kinds of microorganisms.Isolation of Streptococcus b heamolyticus gr. A, Streptococcus pneumoniae, E.coli, Proteus mirabilisa, Salmonellae enteritidis, Pseudomonas aeruginose i Candide spp confirms bacteremia. Difteroids and Bacillus spp. were isolated only from contaminated hemocultures. Staphylococcus epidermidis is the most frequent contminant and causes bacteremia in the patients with intravascular catheter and patients on intravenous and peritoneal dialysis. A similar finding of α hemolitic streptococcus of viridans group was registered in the patients with subacute and acute endocarditis who had had an intravenous catheter implanted.On the basis of the results obtained, we can conclude that microorganisms from groups of Streptococcus, Staphylococcus, Candida and Enterobacteriaceace family are the most frequent causes of bacteremia. Beside its limiting factors (time of sampling, duration of incubation, possible contamination of samples, number of hemocultures), hemoculture is the only method by which the causes of bacteremia and sepsis can be isolated.
To study the use of blood culture bottles for culturing vitreous specimens in acute-onset postoperative infectious endophthalmitis cases.
Forty-eight eyes of 48 patients with mean age of 66.4 +/- ...14.7 years with postoperative endophthalmitis that occurred within 6 weeks after ocular surgery, presenting from June 2001 to January 2005, were retrospectively studied. Vitreous fluid specimens of these patients were cultured in blood culture bottles. The measured outcome is the yield of positive culture.
Vitreous specimens yielded positive in 34 of 48 (70.8%) eyes with blood culture bottles. The most common organism isolated was Staphylococcus epidermidis in 17.6% (6/34) of cases.
The direct inoculation of vitreous specimens into the blood culture bottles is easy to perform, with a high yield positive rate (70.8%). It may be an alternative to conventional culture media for culturing vitreous specimen in infectious endophthalmitis especially in clinics or hospitals that are unable to have adequate microbiology laboratory facilities.
Introduction: Between 2009 and 2010, the rate of contamination of hemocultures drawn in our emergency department was much higher than the quality standards recommended, so we decided to check the ...extraction procedure of the samples to detect possible faults. We also wanted to study the perception of the nursing staff about the quality of their practice.
Methods: This is a prospective study developed in 2 phases. In the first phase, medical staff observed the extraction of hemocultures. In the second phase, an anonymous test was sent to the nursing staff of the emergency department.
Results: We observed major faults in the extraction procedure. Of the 10 items checked, only 2 had a compliance rate greater than 50%. There were significant differences between test answers and data recovered from observation in 7 items.
Discussion: Several technical deficiencies were observed in the procedure for extraction of blood cultures. This fact partly explains the high rate of contamination found in our emergency department.
The aim of the present study was to investigate the presence of Trypanosoma cruzi in the heart, liver, lung, and kidneys, using hemoculture and PCR analysis, of mice infected with different parasite ...strains during the acute and chronic phases of infection. Parasitemia curves revealed strain-specific biological behaviors. For the Y and JLP strains, the acute phase of infection started at days six and ten post-infection, parasitemia peaked at days seven and 15 post-infection, the chronic phase started at days nine and 28 post-infection, and animals started dying at days 19 and 120 post-infection, respectively. When the two strains were compared, the JLP strain exhibited reduced and slower replication rates associated with a delayed peak of parasitism and reduced parasite burdens. However, parasites were detected in all studied organs using PCR analysis. The capacity of both strains to infect different organs likely influences disease pathogenesis.
The capacity of the polymerase chain reaction (PCR) to detect the DNA of Trypanosoma cruzi was evaluated in 90 blood samples from BALB/c mice infected with T. cruzi cloned stocks of genotypes 19 and ...20 (T. cruzi I) and 39 and 32 (T. cruzi II), and treated with benznidazole. The results from the fresh blood examination, hemoculture, and ELISA allowed to group the treated animals into: cured (TC), dissociated (DIS) and non-cured (NC). The PCR detected T. cruzi DNA in 50.9%, 58.3% and 100.0% of the samples from TC, DIS and NC mice, respectively. These DNA possibly derives from live T. cruzi or from recently lysed parasites, suggests that these animals are in fact not cured. The difference between the PCR results and results obtained using other techniques was statistically significant and independent of the parasite genotype. The PCR described has therefore potential to be used in cure control of treated patients.
Primeiro isolado de Oligella urethralis em duas amostras de sangue periférico detectado por metodologia de monitoração contínua de metabolismo (sistema Bactec®) e identificado pelo sistema ...automatizado Phoenix® (BD System) em paciente com linfoma retroperitoneal com metástase em sistema nervoso central (SNC) no Hospital São Paulo da Universidade Federal de São Paulo (HSP/UNIFESP).
Herein, we have analyzed major biological properties following dual-clone
Trypanosoma cruzi infections in BALB/c mice. Eight
T. cruzi clonal stocks, two of each principal genotype, including genotype ...19 and 20 (
T. cruzi I), hybrid genotype 39 (
T. cruzi) and 32 (
T. cruzi II) were combined into 24 different dual-clone infections. Special attention was given to characterize biological parameters assayed including: prepatent period, patent period, maximum of parasitemia, day of maximum parasitemia, area under the parasitemia curve, infectivity, mortality, and hemoculture positivity. Our findings clearly demonstrated that features resultant of dual-clone infections of
T. cruzi clonal stocks did not display either the characteristics of the corresponding monoclonal infections or the theoretical mixture based on the respective monoclonal infections. Significant changes in the expected values were observed in 4.2–79.2% of the mixtures considering the eight biological parameters studied. A lower frequency of significant differences was found for mixtures composed by phylogenetically distant clonal stocks. Altogether, our data support our hypothesis that mixed
T. cruzi infections have a great impact on the biological properties of the parasite in the host and re-emphasizes the importance of considering the possible occurrence of natural mixed infections in humans and their consequences on the biological aspects of ongoing Chagas’ disease.
Infection is one of the most dangerous complications that can be seen when implanting bone or tendon allografts from a deceased donor. The most common germs isolated are found among the cutaneous ...florae, but sometimes they may be present in the bloodstream as a result of severe injuries suffered before the time of the decease. We present a case of contamination of allografts in a musculoskeletal tissue donor deceased after an accident, whose allografts were contaminated by gastrointestinal microorganisms, probably disseminated through the donor’s blood.
Background. Leptospirosis is a zoonosis with worldwide distribution. In Slovenia, Pomurje is an endemic area. Manifestations of leptospirosis may be observed as different types of disease. The range ...from a short-lived febrile state to a severe disease with renal failure, liver impairment, hemorrhage and fulminant course.Patients and methods. Until year 2001 in the Department of infectious diseases at General Hospital Murska Sobota, only serological methods in diagnosis of leptospirosis had been used. Only in 2002 isolation of leptospires from blood was used. Four cases of confirmed leptospirosis hospitalized in our Department in 2002 were presented with broad spectrum of clinical courses and the significance of cultivation of leptospires from blood in the diagnosis.Conclusions. Because of the protean manifestations of leptospirosis, microbiological tests are essential for confirmatory diagnosis. In case of epidemiological data, clinical course and laboratory markers suggesting the diagnosis of leptospirosis, it is advisible to obtain blood cultures.
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