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•Mesta or ambadi oil having diversified use, is generally extracted from fibre crop kenaf (Hibiscus cannabinus) and roselle (H. sabdariffa) seeds.•Mesta seed yielding 22-24% oil is ...source of monounsaturated and polyunsaturated fatty acids (MUFAs and PUFAs) and have high nutritional value.•The seed oil has various phytotechnological applications, ethnomedicinal uses and pharmacological properties.•ICAR-CRIJAF, India has developed high yielding and stress resistant varieties of both kenaf and roselle.•Future breeding objective is to develop high oil containing mesta lines with low anti-nutritional factors along with increased omega PUFAs content.
Kenaf (Hibiscus cannabinus) and roselle (H. sabdariffa) commonly known as mesta or ambadi in India, primarily considered as fibre crop in addition to their diversified use as vegetables, food supplements, paper pulp, construction material, animal feed, pharmacological agent, biofuel etc. Both kenaf and roselle seeds contain a variety of bioactive phytochemicals like polyphenols and phenolic acids, phytosterols, amino acids, fatty acids, phospholipids etc. that possess great nutritional, phyto-pharmaceutical and therapeutic values. Mesta seed contain 22–24% oil and is considered to have high nutritional value and health promoting properties due to the presence of relatively high amount of monounsaturated and polyunsaturated fatty acids (MUFAs and PUFAs). Kenaf and roselle seeds are also good source of dietary fibers and proteins that have great promise as a feed resource for livestock and value added nutritional foods like defatted seed meal, protein concentrates from defatted seed, seed cake etc. Our institute has developed high yielding and stress resistant varieties of both crops with breeding efforts centered on developing high yielding types with better fibre quality. Recently, identification of high yielding mesta lines with more oil content changed focus toward development of low cyclopropenoid fatty acids (CPFA), epoxy fatty acids, palmitic (saturated) acid content along with increased omega PUFAs content varieties. The present review focuses on the detailed profile of valuable phytochemicals, nutrients, and health promoting attributes as well as phyto-technological progress, nutraceutical functions, medicinal and biological properties of kenaf and roselle seed oil, so as to explore their potential uses as ingredients of functional food and natural pharmaceuticals.
España es un país donde se ha dado una larga tradición de evaluación de la transparencia pública. Desde 2008 Transparencia Internacional España ha venido realizando evaluaciones de la transparencia ...para conjuntos institucionales como las Comunidades Autónomas o los Ayuntamientos, a las que se han sumado otras muchas con mayor o menor difusión e impacto, como Acreditra, el Mapa Infoparticip@, Dyntra, etc. La última propuesta metodológica en sumarse a este listado ha sido la Metodología de Evaluación y Seguimiento de la Transparencia en la Actividad Pública (MESTA), desarrollada por el Consejo de Transparencia y Buen Gobierno. Este sistema de evaluación presentado en 2016 tiene hasta la fecha una tímida aplicación práctica con tan solo cuatro ediciones publicadas por los diferentes organismos de control de la transparencia. Sin embargo, puede convertirse en el sistema de referencia, ya que otros sistemas precedentes parecen estar en desuso. Por ello, merece la pena volver la mirada hacia el mismo, su configuración metodológica y su aplicación práctica, sobre todo, las eventualidades y dificultades que ha atravesado la misma, con el fin de desarrollar propuestas de mejora y perfeccionamiento.
España es un país donde se ha dado una larga tradición de evaluación de la transparencia pública. Desde 2008 Transparencia Internacional España ha venido realizando evaluaciones de la transparencia ...para conjuntos institucionales como las Comunidades Autónomas o los Ayuntamientos, a las que se han sumado otras muchas con mayor o menor difusión e impacto, como Acreditra, el Mapa Infoparticip@, Dyntra, etc. La última propuesta metodológica en sumarse a este listado ha sido la Metodología de Evaluación y Seguimiento de la Transparencia en la Actividad Pública (MESTA), desarrollada por el Consejo de Transparencia y Buen Gobierno. Este sistema de evaluación presentado en 2016 tiene hasta la fecha una tímida aplicación práctica con tan solo cuatro ediciones publicadas por los diferentes organismos de control de la transparencia. Sin embargo, puede convertirse en el sistema de referencia, ya que otros sistemas precedentes parecen estar en desuso. Por ello, merece la pena volver la mirada hacia el mismo, su configuración metodológica y su aplicación práctica, sobre todo, las eventualidades y dificultades que ha atravesado la misma, con el fin de desarrollar propuestas de mejora y perfeccionamiento.
Kenaf (Hibiscus cannabinus L.) is a valuable fiber and medicinal plant from the Malvaceae family. It is an alternative crop that may be a feasible source of cellulose which is economically viable and ...ecologically friendly. This plant is cultivated for its fiber although its leaves and seeds have also been used in traditional medicine in India and Africa for the treatment of various disease conditions. Kenaf fibers are commonly used for paper pulp and cordage, but it is also a promising lignocellulosic feedstock for bioenergy production. The kenaf seed oil can be used for cooking and in different industrial applications. The present paper is an overview on its ethnobotanical and phytochemical properties reported in the literature that we have investigated and its great potential as a valuable multipurpose crop due to numerous uses.
The two varieties of mangosteen (
L.) cultivated in Malaysia are known as Manggis and Mesta. The latter is preferred for its flavor, texture, and seedlessness. Here, we report a complete plastome ...(156,580 bp) of the Mesta variety that was obtained through a hybrid assembly approach using PacBio and Illumina sequencing reads. It encompasses a large single-copy (LSC) region (85,383 bp) and a small single-copy (SSC) region (17,137 bp) that are separated by 27,230 bp of inverted repeat (IR) regions at both ends. The plastome comprises 128 genes, namely, 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The plastome of the Manggis variety (156,582 bp) obtained from reference-guided assembly of Illumina reads was found to be nearly identical to Mesta except for two indels and the presence of a single-nucleotide polymorphism (SNP). Comparative analyses with other publicly available
plastomes, including
,
,
var. Thailand,
,
, and
, found that the gene content, gene order, and gene orientation were highly conserved among the
species. Phylogenomic analysis divided the six
plastomes into three groups, with the Mesta and Manggis varieties clustered closer to
,
, and
, while the Thailand variety clustered with
in another group. These findings serve as future references for the identification of species or varieties and facilitate phylogenomic analysis of lineages from the
genus to better understand their evolutionary history.
One of the biggest rivers in the southern part of the Balkan peninsula - the Mesta River is wellknown for frequent flash floods, especially in the upper river course. As a result of severe storms and ...related heavy rain in mid-July 2019, the Cherna Mesta River flooded, and this resulted in heavy damage to the road infrastructure and water-supply systems. All data indicate that this was not a usual water flood, instead at peak flow, the river carried a huge amount of gravel. Our mapping of erosional and depositional features related to the 2019 event, as well as geomorphological analysis, allows for distinguishing distinct sectors along the river valley. Most hazards are defined in the lower reaches of the Cherna Mesta River, where the processes of channel aggradation and lateral erosion are pronounced. The field analysis of the flood-related deposits indicates the operation of debris flood and hyperconcentrated and water flood processes.
•Diagnostics for MeYVMV in mesta (Hibiscus sabdariffa L. and H. cannabinus L.) by loop-mediated isothermal amplification assay was developed.•LAMP assay developed in this study for the detection of ...MeYVMV in mesta is more sensitive than PCR technique.•The LAMP assay has been optimized using Hydroxy Naphthol Blue (HNB) dye is more suitable for field sample diagnosis and in quarantine.•This is the first report for detection of begomovirus species, MeYVMV in the mucilaginous plant species, kenaf and roselle using LAMP.
A loop-mediated isothermal amplification (LAMP) assay was optimized for the detection of Mesta yellow vein mosaic virus (MeYVMV) in diseased plants of mesta (Hibiscus sabdariffa L.& H. cannabinus L.). The LAMP assay was optimized using a set of six primers targeting the MeYVMV genome and could be completed in 30–60 min at 63 °C. The LAMP amplification results were visualized by adding 1 μl of hydroxy naphthol blue (HNB) dye in a 25 μl LAMP reaction mixture prior to amplification as well as by electrophoresis. The LAMP assay, which detected MeYVMV in a 10−5-fold diluted total DNA, was more sensitive than the PCR assay (10−4-fold dilution). The optimized LAMP assay was able to detect MeYVMV in different parts of the kenaf and roselle plants. Similarly, the optimized PCR assay was also capable of detecting MeYVMV in all the different parts of the kenaf plant but failed to detect the virus in the stem and flower buds of the roselle plant. Validation of the LAMP and LAMP with HNB dye assays revealed that the optimized reactions can be used successfully for the in-situ detection of MeYVMV in field samples and in virus quarantine programs. This is the first report of the detection of the begomovirus species, MeYVMV, in the mucilaginous plant species, kenaf and roselle, using a LAMP assay.
Namen članka je predstaviti kakovost družbene infrastrukture izbranih kratkih oskrbnih prehranskih verig v Mestni občini Ljubljana in njen vpliv na varovanje virov, zmanjšanje odpadne hrane in ...embalaže. V raziskavi smo obravnavali šest ponudnikov, vključenih v kratke oskrbne prehranske verige z zabojčki, skupnim naročanjem in partnerskim kmetovanjem. Presoja objektov s pomočjo smernic trajnostne gradnje BNB (Bewertungssystem Nachhaltiges Bauen) je pokazala slabo kakovost in pomanjkljivo družbeno infrastrukturo. V pogovoru z intervjuvanci smo spoznali, da si želijo urejena in lahko dostopna prevzemna mesta z objekti, ki bi omogočali dobre pogoje za ljudi in živila. Mestna občina Ljubljana ima glede na bližnje podeželje z dobrimi pogoji za ekstenzivno in ekološko kmetijsko pridelavo še veliko možnosti za povečanje samooskrbe s kakovostno domačo hrano, ob tem pa bo morala poskrbeti za razvoj družbene infrastrukture.
Mesta crop biomass is one of the unexplored lignocellulosic feedstocks for second-generation bioethanol production. The compositional analysis of two mesta species, namely Hibiscus sabdariffa and H. ...cannabinus were carried out which revealed that the mesta contains 41.89-46.50% cellulose that can be saccharified to release fermentative sugar. Among different physical and physico-chemical pretreatment methods employed, cold alkali (2%) treatment showed maximum cellulose enrichment (38.71%) and significant lignin removal (27.27% of total). Optimization of saccharification process for pretreated mesta biomass was carried out using Box-Behnken design. Based on the observations, use of 4.02% substrate loading, 15.02 FPU/mL of cellulase from psychrotolerant Aspergillus niger SH3, and 5.01 IU/gds of β-glucosidase from Pseudomonas lutea BG8, led to 533.25 mg/gds sugar release from the pretreated biomass. Simultaneous saccharification and fermentation was carried out using efficient thermotolerant yeast strain Saccharomyces cerevisiae LN that resulted in 4.1 g/L ethanol production. It can be concluded that mesta crop can be a good source for the production of second-generation biofuel and the ethanol yield can be further improved by use of accessory enzymes and co-fermenting yeast.
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