Objective: The objective of the study was to evaluate the genotoxic and mutagenic potentials of Kalanchoe pinnata alcoholic leaves extract.Methods: Single cell gel electrophoresis (COMET assay) was ...adopted to investigate genotoxicity potential of standardized ethanolic leaves extract at the concentration of 10, 50, and 100 μg/ml, respectively, using human lymphocytes.Results: K. pinnata leaves extract did not completely damage DNA in human lymphocytes but low, medium, and high-level damage was found in with positive control. The DNA damage level decreases in low, medium, and high (12±1, 6±1, and 1±1) without positive control at the concentration of 100 μg/ml.Conclusion: It was concluded that the parent material did not induce mutagenic and genotoxic potentials at the concentrations 10, 50, and 100 μg/ml but its metabolites are inducing genotoxicity in various models.
Arheološka izkopavanja, ki so leta 1999 potekala v Ljubljani, v inzuli XXVII Emone, so odkrila ostanek trupa stebra z organskim jedrom, postavljenega na kamnito plinto. V prispevku predstavljamo ...najdbo, njen arheološki kontekst in morebitne analogije, mineraloško-petrografsko sestavo ometa, tip pigmenta ter analizo naravnega kamna, uporabljenega za plinto. Od trupa stebra je ostal le zunanji del iz ometa, nanesenega v štirih plasteh in barvanega z rdečo okro, v notranjosti pa se je ohranil odtis trstičja. Plinta je pravokotni blok, narejen iz lokalnega spodnjejurskega oolitskega apnenca, verjetno z območja Podpeči. Oblika stebra kaže, da bi bil lahko del portika ali peristila, arheološki kontekst pa nakazuje povezavo z bazenom oz. kopališčnim kompleksom v tem delu poznorimske Emone.
Organic Medium Enclosed Trough (OMET) system is a new non-drainable growing technique. The study set out to investigate the interactive effects of OMET and Amaranth species variation on some growth ...attributes, yield, bioactive compounds, and nutrients. Three Amaranth species, A. caudatus, A. cruentus, and A. tricolor, were grown in OMET and non-OMET growing conditions. Growth attributes, irrigation water, bioactive compounds, and nutrients were determined. Non-OMET growing conditions and A. cruentus showed the highest total phenolics and flavonols followed by A. caudatus and A. tricolor, whilst tannins were predominant in A. caudatus followed by A. cruentus and A. tricolor. The use of unsupervised PCA showed major metabolic variation based on Amaranth species and the use of supervised OPLS-DA showed clear metabolic variation based on OMET and non-OMET. OMET and A. cruentus showed the highest plant height, stem diameter, biomass, and some nutrients (%protein, Ca, P, Cu, Se, Zn), followed by A. caudatus based only on growth and biomass, and A. tricolor based only on some nutrients (Mg, K, Mn, and Fe). The use of the OMET system on A. cruentus and A. tricolor can be recommended to mitigate climate change effects on nutritional composition using relatively low levels of irrigation water.