A novel Gram-stain-positive, aerobic bacterium, designated strain DHS C021
, was isolated from a limestone sample collected from the Puding Karst Ecosystem Research Station of Guizhou Province, ...southwest China. This strain developed branched vegetative mycelia, and its aerial mycelia fragmented into rod-shaped spores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised galactose, ribose and mannose. The respiratory quinone was identified as menaquinone MK-9(H4). The major cellular fatty acids were iso-C14 : 0 and iso-C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidyethanolamine, phosphatidylinositol, phosphotidylinositolmannosides and one unidentified phospholipid. The genomic DNA G+C content was 69.8 mol% and 16S rRNA gene sequence analysis showed that the strain belonged to the genus Lentzea and shared highest sequence similarity with Lentzeaalbida CGMCC 4.1727
(98.8 %) and Lentzea waywayandensis CGMCC 4.1646
(98.5 %). However, it could be distinguished from these reference strains based on the low levels of DNA-DNA relatedness (54.5±2.7 and 41.7±3.2 %, respectively). On the basis of morphological, chemotaxonomic and phylogenetic characteristics, and DNA-DNA hybridization data, strain DHS C021
represents a novel species of the genus Lentzea, for which the name Lentzeapudingi sp. nov. is proposed. The type strain is DHS C021
(=CGMCC 4.7319
=KCTC 39694
).
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