Izhodišča: Cirkadiani ritem se uravnava na centralni in celični ravni preko transkripcijsko-translacijskih povratnih zank, ki so natančno uravnavane. Vpliv na uravnavanje cirkadianega ritma imajo ...miRNA molekule, ki skupaj s proteinskimi kompleksi privedejo do utišanja prevajanja določenih celičnih proteinov. Kronična motnja v cirkadianem ritmu lahko privede do maligne transformacije celic. Hepatocelularni karcinom je eden najpogostejših vrst raka pri človeku, pri katerem še vedno ni pojasnjen vpliv različnih signalnih poti na karcinogenezo. Namen študije je bil ugotoviti vpliv transkripcijskih dejavnikov cirkadianega ritma in miRNA na izražanje mRNA in krožnih RNA iz istega genskega lokusa, tj. gena LDLR (LDL receptor).
Metode: Poskus smo izvedli na celičnih linijah Hep G2 in Huh7. Sprva smo v celice s postopkom transfekcije vnesli želene molekule (hsa-miR-17 in cirkadiane transkripcijske faktorje), s katerimi smo želeli vplivati na izražanje genov, ki so nas zanimali. Iz celic smo nato osamili RNA, ki smo jo nato prepisali v komplementarno DNA in izvedli qPCR (kvantitativno verižno reakcijo s polimerazo), na podlagi katere smo sklepali na raven izražanja želenih genov. Rezultate smo računalniško obdelali in jih grafično predstavili.
Rezultati: Z obdelavo rezultatov smo ugotovili, da nobene od treh hipotez nismo uspeli potrditi, saj pri nobenem rezultatu v stopnji izražanja želenih genov nismo ugotovili statistično pomembne razlike (α=0,05).
Zaključki: Kljub temu da sprememb v izražanju genov nismo uspeli potrditi, bi bilo raziskovano področje vredno nadaljnjih raziskav, ki bi trdneje prikazale povezavo. Osrediniti bi se morali na konkretne molekule, s čimer bi pridobili nove tarče za zdravljenje jetrnih bolezni, s katerimi bi izboljšali kakovost življenja bolnikov s HCC. Ugotovili bi lahko tudi nove dejavnike tveganja, na podlagi katerih bi lahko bolje ozaveščali prebivalstvo o preventivi, ki bi zmanjšala incidenco HCC.
"Many of the world's best health researchers are in Ontario, and that's why major breakthroughs are happening here," said John Wilkinson, Minister of Research and Innovation. "Ontario is committed to ...ensuring our researchers have the tools and resources they need to get to the global market first, with breakthroughs that will improve people's lives along with our ability to better detect, treat and ultimately prevent diseases such as cancer. Our government is proud to support this important endeavour." Dr. Brent Zanke is a scientist at the Ottawa Health Research Institute, oncologist at The Ottawa Hospital, associate professor of Medicine at the University of Ottawa and chief scientific officer of ArcticDx. Dr. Tom Hudson is the president and scientific director of OICR. Dr. Steven Gallinger is a senior investigator at the Samuel Lunenfeld Research Institute of Mount Sinai Hospital and is also affiliated with the University Heath Network (UHN) as Head of Hepatobiliary Surgical Oncology. OICR is a centre of excellence, moving Ontario to the forefront of discovery and innovation. It is dedicated to research in prevention, early detection, diagnosis, treatment and control of cancer. OICR is a not-for-profit corporation funded by the Government of Ontario through the Ministry of Research and Innovation. For more information, please visit the website at www.oicr.on.ca.
Apr. 13--With both arms curling inward from a rare nerve reaction to a broken arm she suffered in an icy fall three years ago, teacher Tessa Zanke can't bear the pain of hugging her students. Zanke ...is a teacher in Lexington, Ky., and now has help from a teacher's aide. But district administrators say that because they cannot afford an aide for next year, they won't renew Zanke's contract. Doctors who testified in the trial said Zanke's right arm was broken and didn't heal correctly. She developed a disorder known as reflex sympathetic dystrophy, which results when a signal is sent to the brain to send more blood to an injured area. Her condition has spread to her left arm, causing a continuous burning sensation and hypersensitivity.
Referring to the importance of honouring journalists with the awards, Deborah Zanke states, "The very nature of sexual abuse and exploitation of children is for it to be hidden and never discussed. ...The media's coverage of the issue is vitally important in terms of letting those victimized know they are not alone, putting pressure on individuals and governments to do something, and giving hope that something can be done about these horrendous crimes against children." allege", "I don't want to live with the memory of this all my life" Campbell: "Looks who's watching porn" and "Shouldn't we be fighting
This reflects a subtle paradigm shift. It was thought tumours held the keys to drug resistance. But, said Dr. Brent Zanke, "Maybe that's not the whole story. Maybe it's how a person metabolizes a ...drug and the person's predisposition to toxicity ... and what you have to do is marry the sensitivity that exists within a tumour with a person's intrinsic ability to metabolize a drug."
