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  • Genotoxicity of trivalent chromium in bacterial cell. Possible effects on DNA topology
    Plaper, Andreja ...
    Trivalent chromium is a metal required for proper sugar and fat metabolism. However, it has been suggested that it causes DNA damage in in vitro test systems, although in vivo toxicity has not yet ... been proved. In the present study, the effect of Cr3+ on bacterial cells was tested with the Pro-Tox (C) assay, and its cellular uptake was measured with flame atomic absorption spectroscopy. The potential genotoxicity of Cr3+ was further examined by the study of its influence on a bacterial type II topoisomerase. Cr3+ was shown tocause DNA damage and inhibit topoisomerase DNA relaxation activity, probablyby preventing the formation of the covalent link between enzyme and double helix. In addition, Cr3+ decreases the viability andžor proliferation rate of eukaryotic cells such as murine B16 melanoma cells and human MCF-10A neoT ras-transformed human epithelial cells. The possible implication for Cr3+intake by humans is discussed.
    Vir: Chemical research in toxicology. - ISSN 0893-228X (Vol. 17, no. 7, 2002, str. 943-949)
    Vrsta gradiva - članek, sestavni del
    Leto - 2002
    Jezik - angleški
    COBISS.SI-ID - 1166961

vir: Chemical research in toxicology. - ISSN 0893-228X (Vol. 17, no. 7, 2002, str. 943-949)

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