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  • Ugotavljanje specifičnih BCR-ABL mRNA-zaporedij z načinom obratnega prepisovanja in verižne reakcije s polimerazo pri bolnikih z akutno limfoblastno levkemijo = Detection of specific BCR-ABL mRNA sequences in acute lymphoblastic leukaemia patients by the reverse trancriptase and polymerase chain reaction
    Pajič, Tadej ...
    Background. One of the most frequent chromosomal abnormalities seen in acute lymphoblastic leukaemia is a reciprocal translocation t (9,22)(q34;q11). The molecular consequence of translocation is the ... formation of the BCR-ABL gene onthe Philadelphia (Ph) chromoso- me. Patients with Ph+ ALL have been linked with unfavourable course of the disease. We wanted to introduce molecular genetic technique - reverse transcriptase and polymerase chain reaction as a support in the diagnosis of ALL. Methods. RT-PCR was used to amplify to BCR-ABL cDNA after reverse transcription of the mRNA. PCR for BCR-ABL in the ALL was performed by three separate reactions: one for e1a2, second for b2a2 and/or b3a2 mRNA transcripts and third for a control ABL gene in order to demonstrate adequate cDNA quality. The nested PCR strategy was used to increase the specificity of the method. PCR products were analysed by electrophoresis in agarose gels stained with ethidium bromide and detected under UV light. Results. We analysed samples from 7 patients with acute lymphoblastic leukaemia by RT-PCR. Specific e1a2 transcript was determined in one sample with ALL. We also analysed b2a2 and b3a2 transcripts in 7 ALL and 2KML patients. We established the specific transcript in two of them. Conclusion. We applied the PCR method on blood and bone marrow samples from patients with ALL. The method is suitable for routine work and represents the additional tool that can be used for diagnosis and improved estimate success in the medical treatment of patients with ALL.
    Vir: Medicinski razgledi. Supplement. - ISSN 0353-3484 (Letn. 39, suppl. 5, 2000, str. 13-23)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2000
    Jezik - slovenski
    COBISS.SI-ID - 12323801

vir: Medicinski razgledi. Supplement. - ISSN 0353-3484 (Letn. 39, suppl. 5, 2000, str. 13-23)

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