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Določanje kompleksa katepsin B/ cistatin C v bioloških vzorcih in njegova vloga pri napredovanju raka : magistrso delo = Detection of cathepsin B/ cystatin C complex in biological samples and its role in cancer progresion : master thesis = IrenaZore, IrenaA sandwich-type ELISA was developed for assessment of a complex between cathepsin B (CB) and cystatin C (CC) in sera. The assay is based on a combination of two monoclonal antibodies, first one (3E1) ... - catching Ab, was raised against CB and the second one (1A2)-detecting Ab, conjugated with horseradish peroxidase, against CC. Only CBžCC complex, cross-linked and non cross-linked, was able to evoke the signal in ELISA. Detection limit of the assay was 31.25 nmolž1 and the working range between 31.3 - 400 nmolž1. The within and between-run coefficient of variance (CV) varied from 4.7% to 9.4% and from 11 % to 12.8%, respectively, indicating satisfactory reproducibility of the method. The content of CBžCC complex was determined in sera of 133 healthy controls, 32 patients with benign lung diseases, 148 with lung and 32 with colorectal cancer. CBžCC complex was significantly less abundant in sera of patients bearing malignant lung and colorectal tumors than in those with benign lung diseases or healthy controls (p<0.001). In colorectal cancer sera its level was significantly higher in early Dukesć stages A and B than in advanced stages C and D (p=0.02). Our results support the hypothesis of hindered inhibitory capability between KB and CC during cancer progression.Vrsta gradiva - magistrsko deloZaložništvo in izdelava - Ljubljana : [I. Zore], 2000Jezik - slovenskiCOBISS.SI-ID - 2180116
Avtor
Zore, Irena
Drugi avtorji
Kos, Janko, 1959-
Teme
Neoplasms |
Diagnosis |
Cathepsin B |
Blood |
Cystatins |
Blood |
Enzyme-linked immunosorbent assay |
Methods |
Novotvorbe |
Diagnostika |
Katepsin B |
Kri |
Cistatini |
Kri |
Encimsko vezan imunosorbentni test |
Metode |
medicinska onkologija |
diagnostika, laboratorijska
Knjižnica | Signatura – lokacija, inventarna št. ... | Status izvoda |
---|---|---|
MF, Centralna medicinska knjižnica, Ljubljana | magistrske naloge ZORE Irena Določanje IN: 020000834 |
prosto - na dom, čas izposoje: 1 mes. |
Nacionalni inštitut za biologijo in BF, Oddelek za biologijo, Ljubljana | BF oddelek za biologijo mg 577.1 ZORE I. Določanje IN: 0035428 |
prosto - za čitalnico |
Vnos na polico
Trajna povezava
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kos, Janko, 1959- | 04648 |
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