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  • Increase in subcellular GSK-3 clusters in insulin- and adrenaline-treated differentiated rat skeletal muscle fibres
    Fink, Katja, 1991- ...
    Glycogen synthase kinase 3 (GSK-3) plays an important role in m etabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation . The aim of the present study ... was to study the effect of insulin and adrenaline on GSK-3 localisatio n in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully diffe rentiated muscle fibre by immunofluores- cence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of si ngle central optical section of t he muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average siz e were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linea rly related to their quantity. We conclude that subcellular GSK-3 in isolate d skeletal muscle fibres is lo calized in clusters and clustering increased after stimulation with insulin and/or adrenaline.
    Vir: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 39, no. 1, Mar. 2020, str. 25-32, III)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2020
    Jezik - angleški
    COBISS.SI-ID - 27393795

    Povezava(-e):

    https://www.ias-iss.org/ojs/IAS/article/view/2356/1118
    Digitalna knjižnica Slovenije - dLib.si
    DOI

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