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  • Insulin regulation of proliferation involves activation of AKT and ERK 1/2 signaling pathways in vascular smooth muscle cells
    Isenović, Esma R. ...
    This investigation used primary cultured rat vascular smooth muscle cells (VSMCs) to examine the effects of insulin (INS) on proliferation of VSMCs. In this study, protein kinase B (Akt) and p42/44 ... mitogen-activated protein kinase(ERK 1/2) signaling pathways in mediating the mitogenic actions of INS in VSMCs was investigated. Incubation of a rat VSMCs with INS (100 nM) for 10 min. resulted in an increase of Akt phosphorylation by 6-fold (P<0.001) and ERK1/2 phosphorylation by 3-fold (P<0.001). Pretreatment for 15 min with 10 Mof P13 K/Akt inhibitor LY294002 or with 20 M inhibitor of ERK 1/2 PD98059 significantly reduced INS-stimulated Akt and ERK 1/2 phosphorylation by 76% and by 75%, respectively. Incubation of a rat VSMCs with INS resulted in an increase of VSMC proliferation (CONT=100%, INS=187 13%, P<0.001.) The effect of INS on VSMC proliferation was significantly reduced by 68% by pretreatment with LY294002 (P>0.01) and by 71% (P>0.01) by pretreatment with PD98059. These results indicate that INS acts through Akt and ERK 1/2 signaling pathways to up-regulate proliferation of VSMC's.
    Vir: 10th European congress of endocrinology, 3 - 7 May 2008, Berlin, Germany : abstract book (Str. P652)
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2008
    Jezik - angleški
    COBISS.SI-ID - 33910789

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