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  • Characterisation of an in vitro aged cell culture model
    Bunc, Marko, farmacevt ...
    The present study aims to provide a detailed protocol to obtain an aged cell culture model, which is conveniently characterised in terms of morphology, metabolism, and key biochemical markers of ... senescence. Commercial Human Dermal Fibroblasts (HDF) (67-year-old female Asian donor) were cultured over a span of 35 passages to determine at what passages they express the phenotypes found in vivo in the geriatric population. The growth rate and density (counting, Trypan Blue) at confluence declined 4- and 3-fold, respectively. During 15 passages in vitro ageing the fibroblast metabolic capacity (XTT) was heavily reduced (by 75%), while conversely the content of cellular proteins (SRB) slightly increased (2 fold). Senescent cells (SA-%-gal positive) were continuously emerging from 0 to 20%. Freezing/thawing at this stage restored metabolic performance to original values which declined at a similar rate as before. Passages 5 - 10 of our selected aged HDFs were found to mimic in vivo tissue of older adults to the greatest extent.
    Vir: Farmacia. - ISSN 0014-8237 (Vol. 67, iss. 2, 2019, str. 240-247)
    Vrsta gradiva - članek, sestavni del
    Leto - 2019
    Jezik - angleški
    COBISS.SI-ID - 4698993

    Povezava(-e):

    http://farmaciajournal.com/issue-articles/characterisation-of-an-in-vitro-aged-cell-culture-model/

    Prost dostop


    DOI

vir: Farmacia. - ISSN 0014-8237 (Vol. 67, iss. 2, 2019, str. 240-247)

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