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  • Pereira, Lilia Castro; Soriano, Leonardo; de Oliveira, Carolina Rossi; Rodrigues, Paulo Hercílio Viegas; Martinelli, Adriana Pinheiro

    Methods in molecular biology (Clifton, N.J.), 2024, Letnik: 2759
    Journal Article

    The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth. TIS offer advantages such as accelerated multiplication processes, uniform production, and cost reduction. This process has shown promise in meeting the growing demand for alstroemeria plants in the market. This chapter describes a specific protocol for temporary immersion bioreactor micropropagation of the "Albatroz" cultivar, with the potential for large-scale automation.