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Fan, Erkang; Baker, David; Fields, Stanley; Gelb, Michael H; Buckner, Frederick S; Van Voorhis, Wesley C; Phizicky, Eric; Dumont, Mark; Mehlin, Christopher; Grayhack, Elizabeth; Sullivan, Mark; Verlinde, Christophe; Detitta, George; Meldrum, Deirdre R; Merritt, Ethan A; Earnest, Thomas; Soltis, Michael; Zucker, Frank; Myler, Peter J; Schoenfeld, Lori; Kim, David; Worthey, Liz; Lacount, Doug; Vignali, Marissa; Li, Jizhen; Mondal, Somnath; Massey, Archna; Carroll, Brian; Gulde, Stacey; Luft, Joseph; Desoto, Larry; Holl, Mark; Caruthers, Jonathan; Bosch, Jürgen; Robien, Mark; Arakaki, Tracy; Holmes, Margaret; Le Trong, Isolde; Hol, Wim G J
Methods in molecular biology (Clifton, N.J.), 2008, Letnik: 426Journal Article
The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.
