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  • Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns
    Loh, N D; Bogan, M J; Elser, V; Barty, A; Boutet, S; Bajt, S; Hajdu, J; Ekeberg, T; Maia, F R N C; Schulz, J; Seibert, M M; Iwan, B; Timneanu, N; Marchesini, S; Schlichting, I; Shoeman, R L; Lomb, L; Frank, M; Liang, M; Chapman, H N

    Physical review letters, 2010-Jun-04, Letnik: 104, Številka: 22
    Journal Article

    We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

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