The best solution to the polysaccharide ultrasonic extraction of auricularia is as follows. With aqueous conditions of solid-liquid ratio of 1∶60, 4h extraction time, 40w ultrasonic power, 70°C extraction temperature, extract twice to measure the auricularia polysaccharide extraction rate as 14.95%. Then elute the auricularia polysaccharide with DEAE-Cellulose 52 chromatographic column to get auricularia polysaccharide component AAP -1 which is then separated by Sephadex G-200 gel chromatographic column to get mono component. Set up the swimming training model with increasing intensity. Feed the mouse witsh AAP-1 solution. The result shows that a certain dose of AAP-1 taken can increase the contents of muscle glycogen and liver glycogen and maintain a constant blood sugar. The exhaustion time of the mouse can be elongated and the survival time in anoxic condition will be significantly prolonged. The above shows that auricularia polysaccharide has the function of anti-exercise fatigue.