On May 6, 2009, the X-ray imaging and biomedical application beamline（BL13W1） at Shanghai Synchrotron Radiation Facility（SSRF） officially opened to users, with 8–72.5 ke V X-rays. The experimental station is equipped with four sets of X-ray CCD detectors of different pixel size（0.19–24 μm） for on-line phase-contrast imaging and micro-CT imaging with 0.8 μm spatial resolution and 1 ms temporal resolution. An in vivo microCT experiment for a living insect was realized in 4 s. An X-ray fluorescence detector is equipped for X-ray fluorescence mapping imaging and X-ray fluorescence micro-CT imaging with 50 μm spatial resolution. In order to meet different requirements from the users, several experimental methods, such as X-ray spiral micro-CT, Xray local micro-CT, X-ray fast micro-CT, X-ray grating-based differential micro-CT, X-ray fluorescence microCT and X-ray quantitative micro-CT have been developed, and nearly 60 papers related to those developments for this beamline have been published. Moreover, the beamline has realized the remote fast CT reconstruction,providing a great convenience for the users to process experimental data at their offices. As of August 2014,the beamline has offered the user beamtime of（23 145 h）, from which 232 user papers have been published,including 151 SCI papers and 55 papers with SCI impact factor 〉 3. The quantity and quality of the user paper outcome keep a steady increase. Some typical user experimental results are introduced.