A urease-producing Gram-stain-positive actinobacterium, designated strain T5 T , was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia. The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar. Strain T5 T grew at 15–37 °C, pH 6–11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6–9 and without NaCl. The whole-cell sugar hydrolysate of strain T5 T contained galactose, glucose and ribose. The ll -diaminopimelic acid isomer was detected in the cell wall. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids. The main fatty acids were anteiso-C 17 : 0 , iso-C 16 : 0 , anteiso-C 15 : 0 and iso-C 14 : 0 . Comparative analysis of the 16S rRNA gene sequences indicated that strain T5 T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01 T (99.0 %). The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01 T with 89.4 % average nucleotide identity and 33.7 % digital DNA–DNA hybridization. Phylogeny analyses showed that strain T5 T was closely related to Streptomyces fradiae , Streptomyces lavendofoliae , Streptomyces lichenis , Streptomyces roseolilacinus and Streptomyces somaliensis . Based on these polyphasic data, strain T5 T represents a novel species, for which the name Streptomyces solincola sp. nov. is proposed. The type strain is T5 T (=TBRC 5137 T = DSM 42166 T ).