E-viri
Recenzirano
-
Yamasaki, Seiki; Sawada, Shohei; Komatsu, Sumio; Kawahara, Takeshi; Tsuda, Yutaka; Sato, Toshiyuki; Toratani, Akihisa; Kono, Yoshihito; Higaki, Tadashi; Imamura, Hitoshi; Tada, Yusuke; Akamatsu, Naoaki; Tamagaki, Toshiyuki; Tsuji, Hajime; Nakagawa, Masao
Hypertension (Dallas, Tex. 1979), 2000-August, Letnik: 36, Številka: 2Journal Article
The effects of bradykinin on the regulatory mechanisms of prostacyclin synthesis in endothelial cells were investigated in association with intracellular Ca kinetics, cytosolic phospholipase A2 (cPLA2) activity, and mRNA expression of cPLA2 and prostaglandin H synthase (PGHS) isoforms. Bradykinin enhanced prostacyclin release from endothelial cells time-dependently, but pretreatment with EGTA H-7 or HOE 140 inhibited bradykinin-induced prostacyclin release. Bradykinin increased both the influx of extracellular Ca and Ca release from the intracellular Ca storage sites. These reactions occurred within 5 minutes after bradykinin stimulation. Within 15 minutes, bradykinin activated cPLA2 to 1.3-fold the control level. The constitutive expressions of mRNA of cPLA2, PGHS-1, and PGHS-2 was 87, 562, and 47 amol/μg RNA, respectively. With the stimulation of bradykinin, cPLA2 mRNA increased to 746 amol/μg RNA in 15 minutes, PGHS-1 mRNA increased to 10 608 amol/μg RNA, and PGHS-2 mRNA increased to 22 400 amol/μg RNA in 180 minutes. Pretreatment with cycloheximide superinduced cPLA2 and PGHS-2 mRNA expression but almost completely inhibited PGHS-1. Pretreatment with EGTA had effects similar to pretreatment with cycloheximide in the case of cPLA2 and PGHS-1 but did not affect PGHS-2. These findings suggest that the elevation of cPLA2 activity caused by the increase of intracellular Ca concentration is important in the early phase of bradykinin-induced prostacyclin synthesis and that the mechanisms regulating cPLA2 are different from those regulating PGHS isoforms in endothelial cells.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.