New recombinant strains of Penicillium verruculosum with a high level of expression of homologous endo -β-1,4-glucanase II and heterologous phytase A of A. niger , as well as heterologous endo -1,4-β-xylanase E of P. canescens and phytase A of A. niger , are created. This allows us to obtain highly active feed enzyme preparations (EPs) capable of simultaneously significantly reducing the viscosity of nonstarch polysaccharides (NPSs), as well as increasing the bioavailability of phosphorus and minerals of grain-based feed and, thus, increasing the digestibility of nutrients of agricultural animal and poultry feed. Specific EP activities for specific substrates (xylan, β-glucan, and phytate) are studied and the qualitative and quantitative component composition of new EPs is determined. A recipient strain δnia D, into which heterologous endo -1,4-β-xylanase E of P. canescens is successfully cloned, is obtained based on a new enzyme preparation containing homologous endo -β-1,4-glucanase II and heterologous phytase A of A. niger. In this way, a promising producer of three target enzymes is obtained simultaneously.