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Rchiad, Zineb; Haidar, Malak; Ansari, Hifzur Rahman; Tajeri, Shahin; Mfarrej, Sara; Ben Rached, Fathia; Kaushik, Abhinav; Langsley, Gordon; Pain, Arnab
Cellular microbiology, December 2020, Letnik: 22, Številka: 12Journal Article
Theileria annulata is a tick‐transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours that cause a disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria‐induced leukocyte transformation. Dataset comparisons highlighted a small set of genes associated with Theileria‐transformed leukocyte dissemination. The roles of Granzyme A (GZMA) and RAS guanyl‐releasing protein 1 (RASGRP1) were verified by CRISPR/Cas9‐mediated knockdown. Knocking down expression of GZMA and RASGRP1 in attenuated macrophages led to a regain in their dissemination in Rag2/γC mice confirming their role as dissemination suppressors in vivo. We further evaluated the roles of GZMA and RASGRP1 in human B lymphomas by comparing the transcriptome of 934 human cancer cell lines to that of Theileria‐transformed bovine host cells. We confirmed dampened dissemination potential of human B lymphomas that overexpress GZMA and RASGRP1. Our results provide evidence that GZMA and RASGRP1 have a novel tumour suppressor function in both T. annulata‐infected bovine host leukocytes and in human B lymphomas. Summarising the main findings of this study that identified new players in dissemination and oxidative stress regulation of Theileria‐transformed leukocytes and provided evidence for similar roles for GZMA and RASGRP1 in transcriptionally matched human B lymphoma cell lines.
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in: SICRIS
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