One of the components of biodiesels is ethyl myristate and the study and measurement of its thermal parameters are of great importance. The dual-beam laser thermal lens spectroscopy is used for accurate measurement of thermophysical parameters of ethyl myristate. First, to check the accuracy of our optical setup, thermal lens characteristic time, thermal diffusivity and thermal conductivity of carbon disulfide and benzene were measured at 298 K. There was a reasonable agreement between the measured values and the reference values. Then, the effect of temperature changes on thermal diffusivity and thermal conductivity values of ethyl myristate was studied. The results showed that with increasing temperature from 285 to 358 K, thermal diffusivity decreases from 8.75 to 7.45 × 10−8 m2/s, and thermal conductivity from 0.155 to 0.1432 W/mK. It was also shown that by changing in pump laser energy in the range of 0.06–0.12 joules, both thermal diffusivity and thermal conductivity of ethyl myristate are almost constant.