Silver nanoparticles have been synthesized using green and chemical methods. Mussaenda frondosa ( M. frondosa ) leaf extract and sodium citrate used as reducing and stabilizing agents for the synthesis of silver nanoparticles (AgNPs) in green and chemical methods. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and transmission electron microscopy (TEM). The antioxidant activity of green and chemically synthesized nanoparticles was evaluated by DPPH (1, 1-Diphenyl-2-picrylhydrazyl) assay and observed that green synthesized nanoparticles possess remarkable antioxidant activity compared to chemically synthesized nanoparticles and it can be used for enormous applications in the biomedical field. The present study enlightens the importance of green synthesized AgNPs using M. frondosa leaf extract over the chemically synthesized one.