•Spilanthol green extraction approach.•High purity spilanthol was obtained.•All study conditions have been optimized.•Comprehensive spilanthol extraction efficiency study. Acmella oleracea, a plant with high spilanthol content, can be found in Brazilian north region. Despite the commercial interest of spilanthol for local communities and industries, due to its anesthetic properties, there are few works that have made a chemical study of it and almost none with analytical strategies. In this work, the influence of 4 solvents, namely acetonitrile, methanol, dichloromethane and ethanol, chosen from those mainly reported in published papers and their interaction in the extraction of spilanthol from Acmella oleracea flowers has been studied. The influence of each solvent proportions was discussed, and pure ethanol was selected as the one with better extraction efficiency. A model was built using the spilanthol peak area obtained by a HPLC-DAD system. Using the optimized solvent, it was possible to extract spilanthol from up to 10 g of grounded flowers using 25 mL of the solvent, producing an extract highly concentrated. The spilanthol content in this extract was isolated and characterized by liquid chromatography coupled to a triple quadrupole mass spectrometer. Therefore, this work presents an extensive analytical study of the spilanthol optimum extraction procedure, purification of the standard, validation of the analytical line and evaluation of the extraction efficiency with a recovery test.